Method for evaluating toxicity of polycyclic aromatic hydrocarbons (PAHs) gene by utilizing plant comet assay

A comet experiment, genotoxic technology, applied in measuring devices, material analysis by electromagnetic means, instruments, etc.

Inactive Publication Date: 2015-03-25
NANJING AGRICULTURAL UNIVERSITY
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Problems solved by technology

Comet assay, also known as single cell gel electrophoresis (SCGE), is a highly sensitive genetic toxicity research method, which has been used to detect DNA single-strand and double-strand breaks, alkali Sites, incomplete repair sites, DNA cross-linking and other types of DNA damage; but unfortunately, there is still a lack of related technologies using plant comet experiments to evaluate the genotoxicity of PAHs at home and abroad

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  • Method for evaluating toxicity of polycyclic aromatic hydrocarbons (PAHs) gene by utilizing plant comet assay
  • Method for evaluating toxicity of polycyclic aromatic hydrocarbons (PAHs) gene by utilizing plant comet assay
  • Method for evaluating toxicity of polycyclic aromatic hydrocarbons (PAHs) gene by utilizing plant comet assay

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Embodiment 1

[0022] This embodiment includes the following steps:

[0023] 1) Preparation of contaminated quartz sand: select commercial quartz sand, pass through a 30-mesh sieve, clean the quartz sand and dry it at 105°C. Prepare acetone solution with a certain concentration of phenanthrene and pyrene, add them to quartz sand and mix evenly, then put the treated quartz sand in a fume hood, and after the acetone is completely volatilized, prepare a solution containing 0 to 50 mg / kg of phenanthrene and pyrene of quartz sand.

[0024] 2) Seed cultivation and poisoning treatment: select Vicia faba seeds of the same size, plump and insect-free, soak in 5% sodium hypochlorite for 10 minutes for surface disinfection, and rinse with deionized water. Soak in deionized water at room temperature for 24 hours to make the seeds fully imbibition, and change the water 2 to 3 times during this period. After soaking, the seeds were planted in PAHs-free quartz sand, and cultivated at 20°C for 4 days (14 ...

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Abstract

The invention discloses a method for evaluating the toxicity of a polycyclic aromatic hydrocarbons (PAHs) gene by utilizing a plant comet assay, belonging to the technical field of pollutant toxicity evaluation. The method is characterized in that root tips of contaminated vicia faba are utilized for the plant comet assay; a comet image is analyzed by utilizing Komet Version 6.0 software, wherein the TM value of the comet image and the PAHs contamination concentration have an obvious dosage-effect relationship; the TM value can act as an evaluation parameter for balancing the DNA damage by PAHs, and is directly used for evaluating the toxicity of the PAHs gene. The established method for evaluating the toxicity of the PAHs gene by utilizing the plant comet assay has the characteristics of science, high efficiency, rapidity and accuracy, and is suitable for quantitatively evaluating the toxicity of genes of toxic organisms such as PAHs.

Description

technical field [0001] The invention relates to the technical field of pollutant toxicity evaluation, in particular to a method for evaluating the genotoxicity of PAHs using a plant comet experiment. Background technique [0002] PAHs are a class of persistent organic pollutants produced by incomplete combustion or pyrolysis of organic matter, which have teratogenic, carcinogenic, and mutagenic effects. They are also priority pollutants in the environment and widely exist in soil, water, and the atmosphere. PAHs have strong physiological and genetic toxicity and low natural degradation rate. Once they enter the environment, they pose a persistent threat to the ecosystem and human health. It has been confirmed that PAHs have cytotoxicity, developmental toxicity and genotoxicity to humans and various organisms. It is difficult to accurately reflect the actual toxicity by simply using the concentration of PAHs. Therefore, it is urgent to develop quantitative evaluation techniqu...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): G01N27/447
Inventor 高彦征高曦胡小婕康福星
Owner NANJING AGRICULTURAL UNIVERSITY
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