Method for evaluating toxicity of polycyclic aromatic hydrocarbons (PAHs) gene by utilizing plant comet assay
A comet experiment, genotoxic technology, applied in measuring devices, material analysis by electromagnetic means, instruments, etc.
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[0022] This embodiment includes the following steps:
[0023] 1) Preparation of contaminated quartz sand: select commercial quartz sand, pass through a 30-mesh sieve, clean the quartz sand and dry it at 105°C. Prepare acetone solution with a certain concentration of phenanthrene and pyrene, add them to quartz sand and mix evenly, then put the treated quartz sand in a fume hood, and after the acetone is completely volatilized, prepare a solution containing 0 to 50 mg / kg of phenanthrene and pyrene of quartz sand.
[0024] 2) Seed cultivation and poisoning treatment: select Vicia faba seeds of the same size, plump and insect-free, soak in 5% sodium hypochlorite for 10 minutes for surface disinfection, and rinse with deionized water. Soak in deionized water at room temperature for 24 hours to make the seeds fully imbibition, and change the water 2 to 3 times during this period. After soaking, the seeds were planted in PAHs-free quartz sand, and cultivated at 20°C for 4 days (14 ...
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