Method for detecting pesticide residue pollution of corn

A technology of pesticide residues and inspection methods, which is applied in the direction of measuring devices, instruments, scientific instruments, etc., can solve the problems of incomparability and achieve the effect of simple operation

Inactive Publication Date: 2017-03-15
GUANGXI UNIV
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  • Summary
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Problems solved by technology

However, due to different soil physical and chemical properties (pH, organic matter content, etc.), herbicides in different soils have different bioavailability, and the traditional phytotoxicity indicators (EC50 or IC50) based on total concentration measurement and calculation are usually different due to different soil types. are different and not comparable to each other

Method used

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Examples

Experimental program
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Effect test

Embodiment 1

[0023] (1) Soil collection

[0024] Five kinds of soils were collected from Xiaoshan, Jinhua, Huajiachi campuses in Zhejiang Province, Nanchang in Jiangxi Province and Yichun City in Heilongjiang Province, all of which were 0-20cm topsoil. ℃) for later use.

[0025] (2) Soil treatment

[0026] Take by weighing 200g dry weight of various soils in plastic pots, spray a certain amount of nicosulfuron aqueous solution with a plastic watering can, stir to fully mix nicosulfuron and soil, so that the soil moisture is 70% of the maximum water holding capacity, The final moisture contents are: Xiaoshan soil 24%; Jinhua soil 22%; Jiangxi soil 24%; Huajiachi soil 28%; Heilongjiang soil 30%.

[0027] According to the bioassay preliminary test, the concentration of nicosulfuron in different soils is: Xiaoshan soil 0, 0.25, 0.5, 1, 2, 4 mg / kg; Jiangxi, Jinhua, Huajiachi soil 0, 0.5, 1, 2 , 4, 8mg / kg; Heilongjiang soil 0, 1, 2, 4, 8, 16mg / kg. After the treated soil was transferred to a ...

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Abstract

The invention discloses a method for detecting pesticide residue pollution of corn. The method comprises the following steps: spraying a group of nicosulfuron solutions with gradient concentration into a group of test soil, and sowing corn seeds into the test soil after spraying; one day after spraying, collecting in-situ porous water of the soil, and testing the concentration of nicosulfuron in the in-situ porous water; performing culture for multiple days, and testing the height of corn plants; finally performing fitting so as to obtain a dosage effect curve equation of the height of the corn plants and the concentration of the nicosulfuron in the in-situ porous water, and the concentration IC50 of the nicosulfuron in the in-situ porous water when the corn plant height inhibition rate is 50%; testing the concentration of the nicosulfuron in the in-situ porous water of the soil, and predicting the plant height inhibition rate of the corn planted in the soil according to the dosage effect curve equation. The method is simple and rapid to operate, and the plant toxicity of the nicosulfuron in the soil to be tested can be rapidly predicted according to the dosage effect curve equation of the nicosulfuron in the soil and the concentration of the nicosulfuron in the in-situ porous water of the soil to be tested.

Description

technical field [0001] The invention relates to the field of pesticide safety evaluation, in particular to a method for testing residual pesticide pollution in corn. Background technique [0002] Nicosulfuron is a sulfonylurea herbicide variety discovered by Ishihara Sangyo Corporation in Japan and jointly developed with DuPont in 1987-1988. It was sold in the United States under the trade name Accent in 1991 and registered for sweet corn in 1996. Nicosulfuron has been in my country for more than ten years, because of its low dosage and good effect, it is very popular. [0003] The agent is a systemic sulfonylurea herbicide, which can be absorbed through the stems, leaves and roots of plants and quickly conduct in the xylem and phloem. By inhibiting the activity of acetolactate synthase, it hinders the synthesis of branched-chain amino acids, thereby affecting plant cells. Divide, causing the weed to stop growing and eventually die. Weeds will stop growing soon after absor...

Claims

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Application Information

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IPC IPC(8): G01N30/02
CPCG01N30/02G01N2030/027
Inventor 周奉椿卢培坤罗茂松
Owner GUANGXI UNIV
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