35 results about "Dose-effect curve" patented technology

The invention relates to the field of activity detection of biological medicine, in particular to a biological activity determination method for an anti-PD-L1 monoclonal antibody. According to the method, a PD1/PD-L1 interruption detection system is used, binding of PD1 and PD-L1 protein is interrupted when the anti-PD-L1 monoclonal antibody is bound with PD-L1 expressed by target cells, a transcription factor NFAT is activated through signal transduction, expression of luciferase is started, the expression quantity of luciferase is positively correlated to the biological activity of the anti-PD-L1 monoclonal antibody, the chemiluminescence value of the anti-PD-L1 monoclonal antibody is determined after a cell lysis solution and a luciferase substrate are added, a dose-effect curve is drawn, and accordingly the biological activity of the anti-PD-L1 monoclonal antibody is determined. The method is easy to implement, strong in specificity and high in durability and has great significance on quality control and clinical application of the anti-PD-L1 monoclonal antibody.

The invention provides a method for determining the receptor affinity of a GLP-1 receptor agonist. The method is based on a tool cell line, namely CHO-GLP-1R-CRE-Luc<+>, when a sample to be determined is combined with GLP-1 receptor on the surface of a cell, the receptor-mediated signal cascade reaction can be activated, cAMP-response element (CRE) is activated specifically, the expression of the luciferase reporter gene is promoted, the quantity of luciferase in cytosol is detected for drawing and fitting to obtain a dose-effect curve of acting of the sample and the GLP-1 receptor; and the half effective concentration (EC50) is calculated. By inspecting and optimizing all influence factors in the determining process, the method for determining the receptor affinity of the GLP-1 receptor agonist is finally established. The method has the advantages of being high in specificity, precision and accuracy, good in durability and convenient to operate, and the like. The method can be used for determining the receptor affinity of the GLP-1 receptor agonist, and thus such type of drug can be fast evaluated and screened.

The invention discloses a detection method of anti-tumor activity of dendrobium huoshanense refined polysaccharide. The detection method comprises an in-vitro anti-tumor detection method and an in-vivo anti-tumor activity detection method; the inhibition rate of the dendrobium huoshanense refined polysaccharide on tumor cells is calculated through the in-vitro anti-tumor detection method; a dose-effect curve is drawn according to different concentration inhibition rates, and the half inhibitory concentration IC50 of the tumor cells is calculated; the in-vivo growth inhibition effect of the dendrobium huoshanense refined polysaccharide on a C57BL/6J mouse in-vivo transplantable mouse lung cancer LLC tumor is detected through the in-vivo anti-tumor activity detection method; an in-vitro experiment shows that the dendrobium huoshanense refined polysaccharide has an obvious dose effect on an in-vitro anti-tumor effect; an in-vivo activity experiment shows that the dendrobium huoshanense refined polysaccharide has good anti-tumor activity and a relatively good dose-effect relation; the growth of a mouse lung cancer transplantable tumor can be remarkably inhibited and the dendrobium huoshanense refined polysaccharide has an obvious in-vivo anti-tumor effect; two experiment methods are combined so that the detection of the anti-tumor activity of the dendrobium huoshanense refined polysaccharide is more accurate.

The invention provides a method for detecting a genotoxic agent by the utilization of Pseudorasbora parva and belongs to the field of chemicals safety evaluation and environmental monitoring. The method comprises the following steps: (1) processing Pseudorasbora parva by exposure treatment by the use of a water sample to be tested; and (2) carrying out single cell gel electrophoresis assay by using hepatic cells of Pseudorasbora parva processed after exposure treatment, detecting and counting cell tail length, DNA content and tail moment, and drawing a dose-effect curve. Through comparison between the result and a blank control and a positive control and through the dose-effect relationship, genetic toxicity of the test sample is determined. The detection method of the invention can sensitively and rapidly determine genetic toxicity of chemicals. The invention provides a China native species-based detection method for chemicals genetic toxicity evaluation and environmental genetic toxicity monitoring.

The invention relates to 'a subcloned cell strain TF-1-A2, a preparation method thereof and application' and belongs to the technical field of biology. The preparation method of the stable subcloned cell strain TF-1-A2 includes the steps: domesticating GM-CSF (granulocyte-macrophage colony-stimulating factor) dependent TF-1 cells into NGF (nerve growth factor) dependent strains; cloning the induced NGF dependent TF-1 cells by the aid of methylcellulose semisolid media; picking monoclonal cells from the semisolid media and enlarging and cultivating the monoclonal cells; and preparing a dose-effect curve of each monoclonal cell strain to the NGF and performing screening to obtain the subcloned cell strain TF-1-A2. The subcloned cell strain TF-1-A2 obtained by the preparation method is highly sensitive to the NGF and has fine reactivity and continuous inheritance stability. A TF-1 cell growth method established by the aid of the subcloned cell strain TF-1-A2 is used for externally and quantitatively measuring biological activity of the nerve growth factor, and is high in signal-to-noise ratio, fine in repeatability and superior to an existing method.

The invention relates to the technical field of medicine and provides an application of chromane compound HEF-04 in relaxing blood vessel smooth muscle. In animal in vitro experiment, HEF-04 can relax rabbit in vitro blood vessel smooth muscle shrunk by barium chloride and potassium chloride; the vasodilation effect of chromane compound HEF-04 is not inhibited by the incubation of methylene blue, on the contrary, the blood vessel dose-effect curve shifts left due to higher level of potassium in spasm-induced agent; EC50 value has significant difference before and after the incubation. Documents report that the existence of NO and PGI2 can weaken the generation of EDHF; after NO and PGI2 are inhibited, the effect of endothelium-dependent vascular relaxation caused by EDHF is more prominent, showing that the vascular relaxation effect of HEF-04 is possibly related to the increase of EDHF releasing or caused by the interactions of vascular endothelium relaxing factors.

The invention provides a radiosensitive gene marker and application thereof in X-ray radiation dose monitoring. The radiosensitive gene marker comprises three up-regulation gene markers and two down-regulation gene markers; the up-regulation gene markers include Phlda3, Fgg and Kng1; the down-regulation gene markers include Ptprb and Kti. A dose-effect curve constructed based on the expression level of the radiosensitive gene marker helps determine the range of radiation dose effectively, so that the level of X-ray radiation dose can be monitored effectively to accurately prompt a reference dose of X-ray radiation based on biological effect in order to estimate nuclear radiation damage.

The invention provides a detection technology and method for meat containing animal-derived components. The detection technology and method is based on 3D digital PCR (polymerase chain reaction) technology and fluorescent quantitative PCR technology and comprises the following steps: 1) DNA of a to-be-detected sample is extracted, and a PCR amplification template is obtained; 2) a PCR system is prepared, and 3D digital PCR and fluorescent quantitative PCR amplification are performed, wherein the PCR system comprises the PCR amplification template, a universal primer pair, a fluorescent probe and the like and adopts a dose-effect curve of a pure animal-derived sample standard as the standard curve; 3) the copy number and the percentage composition of the animal-derived components in the to-be-detected sample are calculated according to fluorescence signals produced through the 3D digital PCR. According to the method, a PCR liquid is subjected to smear processing with the 3D digital PCR technology, so that interference by background DNA and a substrate is reduced greatly, and the sensitivity can be as low as one copy; meanwhile, by means of the standard curve, direct processing of follow-up samples is facilitated, and operation steps are simplified greatly.

The present invention relates to a method for detecting a dose of ionizing radiation on human peripheral blood lymphocytes. The method mainly comprises: designing primers and a Taqman-MGB probe according to a lymphocyte pig3 gene expression sequence, and constructing a recombinant vector containing the lymphocyte pig3 gene expression sequence as a standard substance; adopting the 10-fold serial diluted standard substance to carry out real-time fluorescence PCR, and drawing an absolute quantification standard curve; carrying out quantitative determination on the expression levels of the pig3 gene of lymphocytes with different culture times after ionizing radiations with different doses to obtain a dose-effect fitting curve of the radiation dose and the pig3 gene expression level; and carrying out quantitative determination on the expression level of the pig3 gene of lymphocytes with the radiation dose to be detected, and calculating the dose of the ionizing radiation on the lymphocytes requiring detection. According to the present invention, the dose of ionizing radiation on human peripheral blood lymphocytes can be rapidly and quantitatively detected so as to meet requirements of simpleness, rapid quantitation and high throughput, and the advantages can be provided when the large-scale radiation accident occurs.

The invention belongs to the field of environmental science and engineering, and discloses a personal care product acute and chronic dose-effect relationship characterization method. The method mainlycomprises the steps of obtaining a dilution factor microplate test; determining S-shaped and J-shaped dose-effect relationship dilution factors; carrying out dose-effect curve fitting; and drawing athree-dimensional dose-effect curve graph. The method disclosed by the invention is beneficial to researching the dose-effect relationship of commodity type actual environmental pollutants with unknown components and unknown concentrations.