3 results about "Dose-effect curve" patented technology

The invention relates to the field of focused ultrasound, in particular to a parameter positioning method and kit for inhibiting PCOS concurrent epilepsy based on focused ultrasound. The method comprises the following steps: acquiring different power and duty ratio combinations corresponding to a plurality of preset gears; according to the prior experiment data and the biological thermal model, predicting safety intervals of stimulation duration under different gears, and setting a plurality of experiment parameter combinations of different preset doses according to the safety intervals; performing ultrasonic stimulation on the experimental model based on the experimental parameter combination to obtain a biological response result; based on a preset dose effect relationship, according to the biological response result, respectively fitting a dose effect curve under each gear; and determining an optimal dose range of each gear based on the dose effect curve, and performing feasibility evaluation on the optimal dose range to determine an optimal parameter combination of focused ultrasound. On the premise of simple experimental operation, short period and low cost, a relatively accurate and effective ultrasonic parameter range is efficiently determined, and support is provided for personalized medical treatment.

This application relates to the field of focused ultrasound, and more particularly to a parameter localization method and kit for inhibiting PCOS-related epilepsy using focused ultrasound. The method includes: acquiring multiple preset power levels, each corresponding to different combinations of power and duty cycle; predicting the safe range of stimulation duration at different power levels based on prior experimental data and a biothermal model; setting several experimental parameter combinations with different preset doses based on the safe ranges; performing ultrasound stimulation on an experimental model based on the experimental parameter combinations and obtaining biological response results; fitting dose-response curves for each power level based on a preset dose-response relationship and the biological response results; determining the optimal dose range for each power level based on the dose-response curves, and evaluating the feasibility of the optimal dose range to determine the optimal parameter combination for focused ultrasound. This method efficiently determines a relatively accurate and effective range of ultrasound parameters while maintaining simple experimental operation, short cycle time, and low cost, providing support for personalized medicine.

The application discloses a method for evaluating the hypothalamus-pituitary reproductive endocrine axis interference effect of a compound, and belongs to the technical field of chemical environmental health risk evaluation. The hypothalamic neuron cell line and the pituitary gonadotropin cell line are selected as in-vitro evaluation models, the exposure concentration gradient of the compound affecting cell activity is obtained through cell culture and cytotoxicity experiment, the cell exposure experiment of the hypothalamic neuron cell line and the pituitary gonadotropin cell line is carried out at the exposure concentration gradient, the expression amount of a target gene in the cells is determined after the exposure is finished, the dose-effect curve of the compound interfering with the expression of the target gene is obtained according to the exposure concentration gradient and the expression amount of the target gene, and the interference effect of the compound on the hypothalamus-pituitary reproductive endocrine axis is evaluated by using a toxicology priority index ToxPi.