A test method for e-cigarette liquid external cytotoxicity wst-1

Abstract

The invention discloses a WST-1 method for testing in-vitro cytotoxicity of tobacco juice of an electronic cigarette. The method is characterized by comprising the following steps: (1) preparing a laboratory reagent; (2) culturing cells; (3) inoculating cells; (4) contaminating the tobacco juice of the electronic cigarette; (5) dyeing through WST-1; and (6) obtaining a result and analyzing. Compared with the prior art, the method has the characteristics that a method for preparing an electronic cigarette contamination solution by adopting weighing is determined aiming at the characteristic of high density of most electronic cigarette tobacco juice samples, the sensitivity of detection is improved by optimizing the reaction time of the WST-1 dye liquor through an optimization step of a cell inoculation density, an optimal contamination concentration is determined by optimizing an electronic cigarette tobacco juice contamination concentration so that an optimal dosage effect curve can be obtained, and a test step of washing cells a plurality of times is omitted by virtue of WST-1 dyeing; the testing method also has the advantages of faster and simpler operation, higher sensitivity and more stable result.

Description

Technical field [0001] The invention relates to the determination of the cytotoxicity of e-cigarette liquid, in particular to a quantitative determination method based on WST-1 (a water-soluble tetrazolium salt) dye analysis of cell survival rate after e-cigarette liquid is poisoned. Background technique [0002] As people understand that "smoking is harmful to health", governments of all countries have actively adopted and implemented effective legislative, administrative or other measures within the scope of current laws and regulations to prohibit smoking in public places. In 2005, China signed the World Health Organization’s Framework Convention on Tobacco Control. In 2012, the Chinese government issued the "China Tobacco Control Plan (2012-2015)", proposing to create a smoke-free environment and implement a total ban on smoking in public places. Indoor smoking bans have led to the rapid growth of new products such as electronic cigarettes in the markets of various countries...

AI Technical Summary

Problems solved by technology

[0004] At present, the use of WST-1 method to evaluate the cytotoxicity of e-cigarette liquid has not been reported in the literature. It is urgent to establish a method for evaluating the cytotoxicity of e-cigarette liquid based on WST-1 dye, and to investigate the cytotoxicity of commercially available e-cigarette products.

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