Method for testing toxicity of residual nicosulfuron on corn

A technology of nicosulfuron and detection method, which is applied in the field of detection of residual nicosulfuron toxicity to corn, can solve the problems of incomparability, etc., and achieve the effect of simple operation

Active Publication Date: 2012-09-19
ZHEJIANG UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

However, due to different soil physical and chemical properties (pH, organic matter content, etc.), herbicides in different soils have different bioavailability, and the traditional phytotoxicity indicators (EC50 or IC50) based on total concentration measurement and calculation are usually different due to different soil types. are different and not comparable to each other

Method used

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  • Method for testing toxicity of residual nicosulfuron on corn
  • Method for testing toxicity of residual nicosulfuron on corn
  • Method for testing toxicity of residual nicosulfuron on corn

Examples

Experimental program
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Effect test

Embodiment 1

[0025] (1) Soil collection

[0026] The five soils were collected from Xiaoshan, Jinhua, and Huajiachi campuses in Zhejiang, Nanchang in Jiangxi, and Yichun in Heilongjiang. They were all 0-20 cm topsoil. The soil properties are shown in Table 1.

[0027] Table 1 Physical and chemical properties of the tested soil

[0028]

[0029] The collected fresh soil was air-dried and passed through a 2mm sieve and placed at room temperature (20-25°C) for later use.

[0030] (2) Soil treatment

[0031] Take by weighing 200g dry weight of various soils in plastic pots, spray a certain amount of nicosulfuron aqueous solution with a plastic watering can, stir to fully mix nicosulfuron and soil, so that the soil moisture is 70% of the maximum water holding capacity, The final moisture contents are: Xiaoshan soil 24%; Jinhua soil 22%; Jiangxi soil 24%; Huajiachi soil 28%; Heilongjiang soil 30%.

[0032] According to the bioassay preliminary test, the concentration of nicosulfuron in dif...

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Abstract

The invention discloses a method for testing the toxicity of residual nicosulfuron on corn. A group of nicosulfuron solutions, the concentrations of which are gradiently distributed, are sprayed in a group of tested soils, and corn seeds are sowed on the sprayed tested soils; on the first day after spraying, the in-situ pore water of all the soils is collected, and the nicosulfuron concentrationsin the in-situ pore waters are determined; after a plurality of days of culture, the plant heights of corn are determined; finally, a dose-effect curve equation for the plant heights and the nicosulfuron concentrations in the in-situ pore water and the nicosulfuron concentrations IC50 in the in-situ pore water at the corn plant height inhabitation rate of 50 percent are obtained by fitting; the nicosulfuron concentrations in the in-situ pore waters of the soils to be tested are determined, and according to the dose-effect curve equation, the plant height inhabitation rate of the soils plantedwith corn is predicted. The method is easy and rapid to operate, and can rapidly predict the phytotoxicity of nicosulfuron in soil to be tested according to the dose-effect curve equation of the nicosulfuron in the soil and the concentration of the in-situ pore water in the soil to be tested.

Description

technical field [0001] The invention relates to the field of herbicide safety evaluation, in particular to a method for detecting the toxicity of residual nicosulfuron on corn. Background technique [0002] Nicosulfuron is a sulfonylurea herbicide variety discovered by Ishihara Sangyo Corporation of Japan and jointly developed with DuPont in 1987-1988. It was sold in the United States under the trade name Accent in 1991 and registered for sweet corn in 1996. Nicosulfuron has been in my country for more than ten years, because of its low dosage and good effect, it is very popular. [0003] The agent is a systemic sulfonylurea herbicide, which can be absorbed through the stems, leaves and roots of plants and quickly conduct in the xylem and phloem. By inhibiting the activity of acetolactate synthase, it hinders the synthesis of branched-chain amino acids, thereby affecting plant cells. Divide, causing the weed to stop growing and eventually die. Weeds will stop growing soon ...

Claims

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Application Information

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IPC IPC(8): G01N30/02
Inventor 虞云龙刘开林韩玉玲
Owner ZHEJIANG UNIV
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