Electronic cigarette smoke solution cell proliferation toxicity evaluation method

Abstract

The invention relates to an electronic cigarette smoke solution cell proliferation toxicity evaluation method. The method is characterized by comprising the following steps: (1) preparing an electronic cigarette smoke solution; (2) carrying out cell inoculation; (3) contaminating the electronic cigarette smoke solution and doping BrdU (Bromodeoxyuridine); assaying the doped amount of BrdU; (5) resulting and analyzing. Compared with the prior art, the method has the following characteristics that aiming at the characteristic that most of electronic cigarette smoke solution samples are high in density, a method that an electronic cigarette contaminated solution is prepared by adopting mass weighing is determined; by a cell applicability validation step, the method is applicable to a variety of adherent culture cells and can be used for inspecting the cell toxicity to different cell lines of the lung caused by the electronic cigarette smoke solution; the sensitivity of detection is improved through optimizing the doping time of the BrdU and the concentration of cell membrane permeation liquid; the best contaminating concentration is determined through optimizing the contaminating concentration of the electronic cigarette smoke solution, so as to obtain the optimal dose-effect curve. In addition, the method further has the advantages of quickness, simplicity and convenience in operation, high sensitivity and stable results.

Description

technical field [0001] The invention relates to the measurement of cell proliferation toxicity outside of electronic cigarette liquid, in particular to a quantitative measurement method for analyzing the cell proliferation toxicity of electronic cigarette liquid after exposure based on the principle of BrdU incorporation. Background technique [0002] With people's understanding of "smoking is harmful to health", governments of various countries have actively adopted and implemented effective legislative, administrative or other measures within the scope of current laws and regulations to prohibit smoking in public places. In 2005, China signed the WHO Framework Convention on Tobacco Control. In 2012, the Chinese government issued the "China Tobacco Control Plan (2012-2015)", proposing to create a smoke-free environment and implement a comprehensive ban on smoking in public places. The indoor smoking ban has led to the rapid growth of new products such as electronic cigaret...

AI Technical Summary

Problems solved by technology

[0005] At present, the effect of e-cigarette liquid exposure on cell proliferation toxicity has not been reported in the literature. It is urgent to establish a method for evaluating the cell proliferation toxicity of e-cigarette liquid based on BrdU incorporation, and to investigate the cell proliferation toxicity of commercially available e-cigarette products

Images