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Application of aphanalide J to preparation of drugs for treating liver cancer

A technology for the treatment of liver cancer and drugs, which is applied in the application field of Aphanalide J in the preparation of drugs for the treatment of liver cancer, and can solve the problems that there are no reports on the activity of compounds

Inactive Publication Date: 2015-11-25
刘高志
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0003] There is no report on the activity of this compound

Method used

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  • Application of aphanalide J to preparation of drugs for treating liver cancer
  • Application of aphanalide J to preparation of drugs for treating liver cancer
  • Application of aphanalide J to preparation of drugs for treating liver cancer

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1: Isolation, preparation and structure confirmation of Aphanalide J

[0012] The preparation method of Aphanalide J is the same as that reported in the literature (Bioactive Terpenoids from the Fruits of Aphanamixis grandifolia, J. Nat. Prod., 2013, 76, 1191-1195).

[0013] Confirmation of structure: white amorphous powder, molecular formula is C 26 h 34 o 7 , with an unsaturation of 10. H NMR spectrum data δ H (ppm, DMSO-d 6 , 500MHz): H-1 (6.72, d, J=12.5), H-2 (5.85, d, J=12.5), H-5 (2.29, dd, J=13.2, 4.0), H-6 (1.85 , dd, J=16.0, 4.0), H-6 (1.64, ddd, J=16.0, 13.2, 4.0), H-7 (4.61, s), H-9 (2.60, d, J=4.0), H -11 (3.80, q, J=4.0), H-12 (3.50, t, J=6.5), H-15 (4.51, q, J=3.5), H-16 (1.61, ddd, J=12.0, 10.5, 3.5), H-16 (1.71, dt, J=12.0, 5.5), H-17 (3.25, dd, J=10.5, 5.5), H-18 (0.55, s), H-19 (1.30, s), H-21 (7.30, s), H-22 (6.40, s), H-23 (7.45, s), H-28 (1.23, s), H-29 (1.35, s), H-30 (1.48, s), 11-OH (5.00, d, J=4.0), 12-OH (4.91, d, J=6.5), 15-O...

Embodiment 2

[0014] Embodiment 2: Pharmacological action test of Aphanalide J

[0015] 1. Materials and Instruments

[0016] HepG2 human liver cancer cell line was purchased from the Institute of Biochemistry and Cell Biology, Chinese Academy of Sciences. The L02 normal human liver cell line was donated by the Liver Cancer Institute, Zhongshan Hospital, Fudan University. AphanalideJ is self-made, and the HPLC normalized purity is greater than 98%. RPMI-1640 medium and trypsin were purchased from Gibco. Standard calf serum was purchased from Biowest. 3-(4,5-dimethylthiazole-2)-2,5-diphenyltetrazolium bromide (MTT), dimethylsulfoxide (DMSO), trypan blue (TrypanBLue), dichlorofluorescence Diacetate diacetate (DCFH-DA) was purchased from Shanghai Sinopharm Chemical Reagent Co., Ltd. Other commonly used reagents were of analytical grade.

[0017] Microbalance (Mettler-Toledo, Switzerland, METTLRERAE2000). Ordinary desktop centrifuge (Shanghai Anting Scientific Instrument Factory). Digit...

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PUM

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Abstract

The invention discloses application of aphanalide J to preparation of drugs for treating liver cancer and belongs to the field of drugs. The aphanalide J can effectively restrain growth and proliferation of HepG2 cells, a survival rate of cells of an interfered group is obviously reduced in comparison with that of a control group, the decreased degree presents a certain dosage effect trend with the concentration of the aphanalide J, and meanwhile, under the same concentration of the aphanalide J, the survival rate of cells is continuously reduced with the increase of interfering time. At the same time, intervention of aphanalide J with same dosage does not have an inhibiting effect on L02 common hepatic cells and cytotoxicity does not exist. The aphanalide J can further be researched and developed to be used for preparing drugs for treating liver cancer.

Description

technical field [0001] The present invention relates to a new application of compound Aphanalide J, in particular to the application of Aphanalide J in the preparation of drugs for treating liver cancer. Background technique [0002] Yao Zhang et al. isolated and purified the compound Aphanalide J for the first time, and published the results in the famous Journal of Natural Products (Bioactive Terpenoids from the Fruits of Aphanamixis grandifolia, J. Nat. Prod., 2013, 76, 1191-1195). [0003] There is no activity report of this compound yet. Contents of the invention [0004] The object of the present invention is to provide a medical application of Aphanalide J. [0005] The above-mentioned purpose is achieved through the following technical solutions: [0006] The application of Aphanalide J in the preparation of medicine for treating liver cancer, the chemical structural formula of Aphanalide J is as follows, [0007] [0008] Further, the liver cancer is human l...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A61K31/585A61P35/00
Inventor 刘高志
Owner 刘高志
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