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Rare minnow beta type estrogen receptor and use thereof

A technology of estrogen receptor and rare gobi carp, which is applied in the field of molecular biology and environmental biology, can solve the problems of different affinity and achieve the effect of simple operation

Inactive Publication Date: 2009-07-01
RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

For estradiol, ERα and ERβ have similar affinity, but for phytoestrogens and some estrogen antagonists, the affinity for ERα and ERβ is different

Method used

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  • Rare minnow beta type estrogen receptor and use thereof
  • Rare minnow beta type estrogen receptor and use thereof
  • Rare minnow beta type estrogen receptor and use thereof

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Embodiment Construction

[0035] The present invention will be further described below in conjunction with specific embodiments. The following examples are only used to illustrate the present invention, and do not limit the scope of the present invention. The specific experimental conditions that are not specified in the experimental methods in the following examples are implemented according to conventional conditions or according to the conditions recommended by the manufacturer.

[0036] 1. Chemical reagents

[0037] Estradiol (17β-estradiol, E2), 2,4-dichlorophenol (2,4-dichlorophenol) and Dimethyl Sulfoxide (DMSO) were purchased from Sigma (St. Louis, MO, USA) . The estradiol (17β-estradiol, E2) and 2,4-dichlorophenol were separately prepared into DMSO solutions, and then the experimental exposure water was prepared according to the DMSO solution: water 0.005% (volume ratio).

[0038] 2. Domestication of laboratory animals

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Abstract

The invention provides a rare golden carp beta type estrogen receptor and an encoded gene thereof. The invention also provides a method for detecting the chemical toxicity of an aquatic environment and a kit for diagnosing the diseases of aquatics. The method and the kit both relate to the detection of the expression level of the receptor. The invention is applied to detecting the chemical toxicity of the aquatic environment and diagnosing the diseases of aquatics, in particular the piscine genital system diseases, thereby realizing the aim of regulating the piscine reproductive ability. Experimental results show that: the expression of the GrER beta gene has a dosage-effect relationship with the estrogen effect; therefore the method has the advantage of simple, quick and correct operation.

Description

Technical field [0001] The present invention belongs to the fields of molecular biology and environmental biology, and relates to a β-type estrogen receptor and its coding gene, and a method for detecting chemical toxicity of aquatic environment by using the β-type estrogen receptor as a marker and for diagnosis A kit for aquatic animal and plant diseases. Background technique [0002] Estrogen is a steroid hormone, which is converted from androgen catalyzed by aromatase in the body. Estrogen is fat-soluble and has many target tissues and organs, including the reproductive system, bones, and cardiovascular systems. There are currently three types of estrogen found in the human body: estradiol, estrone and estriol. Through research, it is found that estrogen initiates downstream events such as transcription and translation by binding to the corresponding estrogen receptor in the cell, produces corresponding physiological effects, and then regulates a series of life events such as ...

Claims

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Application Information

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IPC IPC(8): C07K14/72C12N15/12C12N15/63C12Q1/68
Inventor 王子健张小艳查金苗饶凯锋
Owner RES CENT FOR ECO ENVIRONMENTAL SCI THE CHINESE ACAD OF SCI
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