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Method for performing biological pre-warning on low-dosage gamma radiation by adopting teratogenesis rate of zebrafish embryos

A technology of zebrafish embryos and gamma radiation, applied in biological testing, material inspection products, etc., can solve problems such as imperceptible, chronic and recessive, unable to effectively and comprehensively deal with radiation pollution, and achieve good accuracy, Easy and fast operation, low cost effect

Active Publication Date: 2016-02-10
NANHUA UNIV
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  • Summary
  • Abstract
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AI Technical Summary

Problems solved by technology

The damage caused by ionizing radiation can be acute, but in most cases it is chronic and recessive, with long-term effects and genetic effects, which are often difficult to detect, so radiation warning must be carried out to prevent problems before they happen
However, the traditional low-dose radiation detection methods cannot quickly, effectively and comprehensively deal with various complex radiation pollution conditions. To evaluate and warn the comprehensive toxicology of low-dose radiation, there is an urgent need for a rapid, sensitive and low-cost detection of environmental toxicity. Biological detection and early warning methods

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment approach

[0037] Zebrafish aged 5-6 months were used for breeding and spawning. Two days before mating, male and female zebrafish were reared in groups in different breeding tanks. On the eve of breeding and spawning, the male and female broodstock were placed in the same mating device at a ratio of 1:2, and the male and female fish were separated by baffles. Control the photoperiod to 14:10 (14 hours of light, 10 hours of darkness). After 10 hours of darkness, remove the partition between the male and female broodstock. The male fish will chase the female fish and bump into the abdomen of the female fish with his body. sperm, the female begins to lay eggs. After the collected zebrafish fertilized eggs were washed clean, they were cultured in a 12-well cell culture plate, with 5 fertilized eggs per well, and 10 wells in each plate for a total of 50 embryos. Pour 10 mL of incubation solution into a 12-well plate , Cultivate at a temperature of 28°C±0.5°C. After the zebrafish fertilize...

Embodiment 1

[0042] Six-month-old zebrafish were used for breeding and spawning. Two days before mating, male and female zebrafish were reared in groups in different breeding tanks. On the eve of breeding and spawning, the male and female broodstock were placed in the same mating device at a ratio of 1:2, and the male and female fish were separated by baffles. Control the photoperiod to 14:10 (14 hours of light, 10 hours of darkness). After 10 hours of darkness, remove the partition between the male and female broodstock. The male fish will chase the female fish and bump into the abdomen of the female fish with his body. sperm, the female begins to lay eggs. After the collected zebrafish fertilized eggs were washed clean, they were cultured in a 12-well cell culture plate, with 5 fertilized eggs per well, and 10 wells in each plate for a total of 50 embryos. Pour 10 mL of incubation solution into a 12-well plate , Cultivate at a temperature of 28°C±0.5°C. After the zebrafish fertilized ...

Embodiment 2

[0045] Six-month-old zebrafish were used for breeding and spawning. Two days before mating, male and female zebrafish were reared in groups in different breeding tanks. On the eve of breeding and spawning, the male and female broodstock were placed in the same mating device at a ratio of 1:2, and the male and female fish were separated by baffles. Control the photoperiod to 14:10 (14 hours of light, 10 hours of darkness). After 10 hours of darkness, remove the partition between the male and female broodstock. The male fish will chase the female fish and bump into the abdomen of the female fish with his body. sperm, the female begins to lay eggs. After the collected zebrafish fertilized eggs were washed clean, they were cultured in a 12-well cell culture plate, with 5 fertilized eggs per well, and 10 wells in each plate for a total of 50 embryos. Pour 10 mL of incubation solution into a 12-well plate , Cultivate at a temperature of 28°C±0.5°C. After the zebrafish fertilized ...

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Abstract

The invention relates to a method for performing biological pre-warning on low-dosage gamma radiation by adopting a teratogenesis rate of zebrafish embryos. The comprehensive toxic effect of the low-dosage gamma radiation on the zebrafish embryos is estimated by calculating the teratogenesis rates of the zebrafish embryos under different conditions of low-dosage gamma radiations according to a dosage-effect relation between the radiation dosages on the zebrafish embryos and the teratogenesis rates via adopting the sensitive characteristic of the zebrafish embryos to the low-dosage gamma radiation, so that a biological pre-warning method is implemented. The method has multiple advantages of simple and fast operation, low cost, high sensitivity, high accuracy, low environmental risk and the like.

Description

technical field [0001] The invention belongs to the technical field of low-dose gamma radiation biological early warning, and in particular relates to a method for low-dose gamma radiation biological early warning by utilizing the teratogenic rate of zebrafish embryos. Background technique [0002] The radionuclides in uranium tailings and waste ores can cause radiation damage to organisms through the ionization and excitation of various rays released by the nuclides, and can also enter organisms and cause internal radiation damage, which is harmful to organisms in the surrounding environment. Including microorganisms, animals, plants, and humans causing long-term potential low-dose radiation hazards. After radionuclides are absorbed and enriched by animals and humans, their ionizing radiation will cause ionization of molecules and atoms in biological tissues, destroy the macromolecular structure in tissues, and threaten health. The damage caused by ionizing radiation can b...

Claims

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Application Information

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IPC IPC(8): G01N33/48
Inventor 丁德馨赵维超胡南龙鼎新冯永富李广悦王永东胡淼
Owner NANHUA UNIV
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