Fusion protein of tumor vascular targeted peptide and novel tumor necrosis factor

A tumor necrosis factor, tumor blood vessel technology, applied in the field of tumor blood vessel targeting peptide novel tumor necrosis factor fusion protein
CN101481418AInactive Publication Date: 2009-07-15FOURTH MILITARY MEDICAL UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Current Assignee / Owner
FOURTH MILITARY MEDICAL UNIVERSITY
Publication Date
2009-07-15
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention belongs to the pharmaceutical biotechnology field, in particular relates to gene cloning, expression of an exogenous gene in a procaryotic cell, purification of target protein, and novel tumor necrosis factor fusion protein of tumor blood vessel targeted peptide. The invention is characterized in that a small bacteriophage peptide capable of being specifically combined with a blood vessel with gastric cancer is obtained by applying a bacteriophage peptide library in vivo screening technology, and is fused into Escherichia coli together with a novel human tumor necrosis factor alpha (rmhTNF) to obtain high-level expression, the target protein is purified by ammonium sulfate salting-out or an anion-cation exchange method, and the obtained target protein is subject to an activity test. The target protein has the advantages of high efficiency and low toxicity of a novel recombinant human tumor necrosis factor, and can gather in a new blood vessel of a gastric cancer tissue, thus further lowering the clinical dosage for the human tumor necrosis factor, improving the dosage-effect ratio and lowering the toxic and side effect during gastric cancer treatment.
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Description

technical field

[0001] The invention belongs to the technical field of medicine and biology, and specifically relates to gene cloning, expression of exogenous genes in prokaryotic cells, purification of target proteins, and novel tumor necrosis factor fusion protein of tumor blood vessel targeting peptide. Background technique

[0002] Related research and existing problems of tumor angiosuppressive therapy:

[0003] Tumor angiogenesis is a prerequisite for rapid tumor proliferation, and inhibiting tumor angiogenesis is an important approach for tumor treatment. At present, the tumor vasculature suppression therapy mainly includes the following approaches: ①Blocking the regulatory factors that promote angiogenesis. Vascular growth stimulating factors such as VEGF are the most commonly found pro-angiogenic growth factors in tumor cells, and they are also the main target molecules in vascular therapy. ②Enhance the regulatory factors that inhibit the growth of blood vessels. ...

Claims

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