Method for predicting acute joint toxicity of three pesticides to photogenic bacteria

Abstract

The invention discloses a method for predicting acute joint toxicity of three pesticides to photogenic bacteria, which aims to overcome problems that a conventional toxicology acute joint toxicity evaluation technique is large in testing workload and quantitative evaluation and acute joint toxicity action prediction methods are not available. The method for predicting the acute joint toxicity of three pesticides to photogenic bacteria comprises the following steps: (1) performing pretesting, namely confirming the testing concentration of different pesticides in official tests, wherein the step of performing pretesting, namely confirming testing concentrations of different pesticides in official tests comprises the following steps: (1) primarily confirming high, medium and low photogenic bacteria toxicity concentration ranges of a single pesticide; (2) establishing a dosage-effect equation that y is equal to f(x)(x belongs to [C,C']) of the single pesticide; (3) confirming the testing concentrations of different pesticides in the BBD tests; (2) confirming testing schemes through three-factor three-level center combined testing design (BBD); (3) performing official testing, namely, testing the relative light emission inhibition rates of different test groups of photogenic bacteria; and (4) establishing a model to predict the acute joint toxicity of the three pesticides to the photogenic bacteria.

Description

technical field [0001] The invention relates to a method for predicting the acute biological toxicity of compounds, more precisely, the invention relates to a method for predicting the acute combined toxicity of three pesticides to luminescent bacteria. Background technique [0002] The toxicity test of luminescent bacteria is an effective method for microbial detection of environmental pollution and the toxicity of detection substances, which emerged after the 1970s. The biotoxicity test of luminescent bacteria using modern photoelectric detection means is one of the main biotoxicity determination methods in modern toxicology research. The International Organization for Standardization (ISO), the United States and China have promulgated relevant standards for the evaluation of water quality toxicity by luminescent bacteria. [0003] There are many kinds of pollutants in the environment, and the joint toxicity of compounds has been widely concerned. At present, the combine...

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Problems solved by technology

[0006] The technical problem to be solved by the present invention is to overcome the problems of the existing toxicological acute combined toxicity evaluation technology, such as large test...

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