Solanum melongena phomopsis rot resistance rapid identification method

An eggplant brown scab disease and identification method technology, applied in the field of disease resistance identification and rapid identification of brown scab resistance, can solve the problem of lack of operation methods and identification standards, affecting the authenticity of resistance, differences in resistance identification, etc. problems, to achieve the effect of easy inoculation environmental conditions, control of inoculation environmental conditions, and simple operation

Inactive Publication Date: 2014-11-05
WUHAN VEGETABLE RES INST
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Problems solved by technology

, this method is not only time-consuming and labor-intensive, but also due to some factors such as environmental changes and cultivation conditions, there are large differences between the resistance identification replicates, thus affecting the authenticity o...

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  • Solanum melongena phomopsis rot resistance rapid identification method

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Embodiment Construction

[0019] A total of more than 50 eggplant germplasm resources of different types were planted and managed under normal cultivation. Normal commercial fruits were taken for indoor inoculation tests when the results were in full bloom, and two highly pathogenic strains (numbered PV-F2 and PV-F5) were used for the inoculation test. Inoculate, observe and measure the test results after ten days, take the pathogenicity data of the strains with strong pathogenicity as the final test results of the variety, repeat the test at least twice for each variety, and carry out the variety resistance according to the eggplant brown spot resistance grading standard in Table 1 Identification. The test was carried out for two consecutive years, and the final resistance results were averaged over the two years.

[0020] The specific experimental method is as follows:

[0021] 1. First collect the normal commodity eggplant fruits of each variety, take 10 eggplant fruits randomly for each variety, c...

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Abstract

The invention discloses an solanum melongena phomopsis rot resistance rapid identification method comprising the following steps: (1) solanum melongena phomopsis rot strains are inoculated on a culture medium and are activated; the solanum melongena phomopsis rot strains are inoculated on a new culture medium; and well-cultured bacterial masses are selected for inoculation; (2) a wound is cut on a solanum melongena fruit sample to be tested; inoculation is carried out wherein a bacterial mass is flatly placed under solanum melongena fruit epidermis from the wound; the surface of the wound is covered by using a sterile absorbent cotton ball; sterile water is sprayed on the surface of the solanum melongena fruit; and the solanum melongena fruit is sealed by using a plastic wrap, such that the solanum melongena fruit is kept hydrate; (3) the solanum melongena fruit is placed under room temperature, and solanum melongena fruit epidermis lesion diameters d1 and d2 are counted with a decussation method; a lesion area is calculated; and the resistance level of the solanum melongena against phomopsis rot is divided according to the lesion area size. With the method provided by the invention, solanum melongena phomopsis rot resistance identification can be rapidly completed indoor. The method has the advantages of simple operation and short period. The operation is carried out indoor, such that inoculation environmental conditions are easy to control.

Description

technical field [0001] The invention relates to an identification method for disease resistance, in particular to a rapid identification method for brown spot disease resistance of eggplant, and belongs to the field of agricultural biology. Background technique [0002] Eggplant (Solanum melongena), as a worldwide vegetable, is often seriously harmed by Phomopsis vexans. In recent years, a large number of this disease has been reported in almost all areas where eggplant is cultivated in my country. Numerous studies have shown that the prospect of chemical control in the control methods against this pathogen is not ideal, and the most economical and effective measure is to cultivate varieties with high resistance to brown spot disease. [0003] Among them, researchers are faced with a serious problem, which is how to quickly identify whether the new brinjal variety is resistant to brinjal disease. The traditional method is to analyze the resistance of eggplant varieties thro...

Claims

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Application Information

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IPC IPC(8): A01G7/00
Inventor 张敏谈太明徐长城谈杰黄树苹王春丽
Owner WUHAN VEGETABLE RES INST
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