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Indoor identification method for anti-phomopsis blight

A technology for indoor identification of Solanaceae spp., applied in the direction of microorganism-based methods, microorganism measurement/inspection, biochemical equipment and methods, etc., can solve the problems of unsatisfactory comprehensive conditions, increased costs, residues, etc., and achieve anti-bacteria Disease screening conditions are easy to control, easy to operate, and reliable results

Active Publication Date: 2014-11-26
WUHAN SHUBO AGRI TECH CO LTD
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Problems solved by technology

[0004] At present, the prevention and control methods of eggplant brown spot disease are mainly through cultivation and chemical control methods, but the overall situation is not very ideal. The economical measure is to cultivate varieties with high resistance to brown spot disease. Screening resistant varieties is one of the main methods for effective and urgent prevention and control of eggplant brown spot disease, which can greatly improve the economic benefits of eggplant production

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  • Indoor identification method for anti-phomopsis blight
  • Indoor identification method for anti-phomopsis blight
  • Indoor identification method for anti-phomopsis blight

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Embodiment 1

[0031] The indoor identification method of resistance to brown spot disease of eggplant comprises the following steps:

[0032] A. Eggplant seedling cultivation and planting

[0033] Select different types of eggplant varieties as identification objects. These varieties are all existing cultivars but not limited to these varieties. The tested varieties include: Chuangfeng Sanyue eggplant, Black Angel Zaoqie F1, Xindachang, Meiqiu No. 1, Selected Purple Canned Eggplant, White and Tender Changbai Eggplant F1, Wushan No. 3 Zihong Long Eggplant, Yuzao No. 4 Eggplant, No. 1 Yang Eggplant, Siji Green Eggplant Overlord (these eggplant varieties are marketed varieties, purchased from Wuhan Seed Industry Branch of Vegetable Science and Technology Development Corporation).

[0034] The eggplant seeds to be tested were sown in a hole tray with a sterilized substrate (peat soil, vermiculite and pastoral soil mixed in a volume ratio of 1:1:1, sterilized with formalin solution), and the se...

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Abstract

The invention belongs to the technical field of gardening crop disease resistance identification, and concretely relates to an indoor identification method for anti-phomopsis blight. The method comprises the steps of seedling growing and colonization of eggplant, preparation of a fluid suspension for phomopsis blight pathogen, pathogen invasion treatment and pathogenesis investigation. According to the invention, eggplant fruit is selected as an identification material indoor, a self made cross-shaped cutter is used for making cross wound on the eggplant, a filter paper sheet containing the fluid suspension for phomopsis blight pathogen spore is used for invasion treatment, and then pathogenesis investigation is carried out, and thereby the appropriate concentration of the fluid suspension for phomopsis blight pathogen spore as well as the length and depth of the cross wound can be cleared. Compared with large land inoculation, factors such as inappropriate climate condition and other insect disease influence in environment can be avoided, and the method has the advantages of easy control of disease resistance screening condition, consistent standard, rapid pathogenesis, simple operation and reliable result, a lot of manpower and material resources can be saved, and the method is especially suitable for identification of large batch of phomopsis blight resistance for eggplant breeding material.

Description

technical field [0001] The invention belongs to the technical field of disease resistance identification of horticultural crops, and in particular relates to an indoor identification method of eggplant resistance to brown spot disease. Background technique [0002] Eggplant (Solanum melongena L.), also known as Luosu, originated in Southeast Asia and India. It was introduced to China as early as the 4th and 5th centuries AD. It has a history of about 1500 years. It is one of the main fruits and vegetables in spring and summer in my country. Eggplant cultivars are divided into three varieties: round eggplant, long eggplant and dwarf eggplant; divided according to the color of the peel, including three types of purple, white and green; divided by maturity, including three types of early maturity, middle maturity and late maturity. At present, the national eggplant cultivation area is 800,000 hm 2 , accounting for about 50% of the world's total cultivated area (Wu Renfeng, 201...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12Q1/18C12N1/14C12R1/645
Inventor 吴仁锋杨绍丽
Owner WUHAN SHUBO AGRI TECH CO LTD
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