Phosphorus-dissolving pseudomonas putida L13 and fermentation process thereof

Abstract

The invention belongs to the technical field of agricultural microbiology, and particularly relates to the separation screening and fermentation cultivation of phosphorus-dissolving pseudomonas putida L13. A phosphorus-dissolving pseudomonas putida L13 strain with the capacity of dissolving phosphorus is obtained through separation, and the preservation No. is CCTCC No.2010342. The fermentation process comprises the following steps of: (1) taking L13 bacterial strain slope seeds for streak cultivation on an LB (lysogeny broth) culture medium flat plate to obtain first-level seeds; (2) inoculating a first-level seed single bacterial colony into an LB culture medium shake flask to make OD (optical density) 600 be 2.0 to obtain second-level seeds; (3) inoculating the second-level seeds into a shake flask for fermentation cultivation to obtain the pseudomonas putida; or inoculating the second-level seeds into a fermentation tank so as to obtain the pseudomonas putida through optimized cultivation. The pseudomonas putida strain disclosed by the invention grows rapidly, the yield of the pseudomonas putida is high, and the viable count can reach more than 10 billions. The phosphorus-dissolving amount of the phosphorus-dissolving pseudomonas putida in a PKO (Pikovskaya') culture medium in which 1-3g / L of calcium phytate is the unique phosphorus source can reach 120-220mg / L, and the phosphorus-dissolving amount of the phosphorus-dissolving pseudomonas putida in a PKO culture medium in which 4-6g / L of tricalcium phosphate is the unique phosphorus source can reach 300-500mg / L.

Description

technical field

[0001] The invention relates to the field of microorganisms, in particular to a strain of Pseudomonas putida phospholyticum and its fermentation process. Background technique

[0002] Only 1% to 5% of the total phosphorus source in the soil exists in the form of soluble phosphorus that can be used by plants. Due to the adsorption effect of the soil, about 90% of traditional chemical fertilizers will be fixed by the soil soon after being applied to the soil and become insoluble phosphorus that cannot be used by plants, and only a small part of the soluble phosphorus that can finally exist in a stable form. Therefore, after the 1980s, with the continuous development of agriculture, the soluble phosphorus in the soil decreased to a considerable extent, while the reduction in the total inorganic phosphorus sources in the soil was relatively small.

[0003] There are a variety of beneficial microbial flora in the rhizosphere of crops, we call it plant growth prom...

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