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Restoration method special for oryza sativa L. polluted by polycyclic aromatic hydrocarbon phenanthrene

A polycyclic aromatic hydrocarbon phenanthrene and rice technology, which is applied in the field of agricultural pollution remediation, can solve problems such as pollution, and achieve the effects of reducing pollution, being easy to control and easy to operate.

Inactive Publication Date: 2016-09-07
NANJING NORMAL UNIVERSITY
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

Using endophytic bacteria fermentation broth with growth promotion, efficient degradation of agricultural soil pollution phenanthrene and broad-spectrum rice endogenous colonization to make rice pollution remediation agent to solve the problems existing in rice polycyclic aromatic hydrocarbon phenanthrene pollution

Method used

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  • Restoration method special for oryza sativa L. polluted by polycyclic aromatic hydrocarbon phenanthrene

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Experimental program
Comparison scheme
Effect test

Embodiment 1

[0024] The preparation process is as figure 1 shown

[0025] (1) activate the broad-spectrum plant endophytic fungus (Phomopsis sp.) HQ285146 on the slant respectively, and use the bacterial strains for fermentation to prepare the seed liquid respectively (medium composition (per L): 200g potato juice, 20g glucose, 20g agar), then press 10% of the inoculum was fermented separately (PDA: 200g potato juice / L, 20g glucose / L), 28°C, 150rpm fermentation for 7 days.

[0026] (2) Harvest the fermentation products respectively, dilute them with water, the volume ratio of the fermentation products and water is 1L:50L, and finally prepare the rice phenanthrene pollution remediation preparation.

Embodiment 2

[0028] The rice phenanthrene pollution remediation agent made by the above-mentioned Phomopsis sp. is applied to the rice culture solution containing phenanthrene (50mg / L), and the concentration is 10ml / L, and the rice phenanthrene pollution remediation agent is added after 9 days. In the treatment group, the degradation rate of phenanthrene in the culture fluid was 97%, while in the control group, the degradation rate of phenanthrene was 39%, and the content of phenanthrene in the rice culture fluid decreased significantly.

Embodiment 3

[0030] Soak the phenanthrene-contaminated rice (leaves 116mg / kg; root 1448mg / kg) in the above-mentioned rice phenanthrene pollution restoration agent, then take it out and plant it in the rice culture solution. After 30 days, the degradation rate of phenanthrene in the rice leaves of the treatment group was 88%, the root is 98%; the degradation rate of phenanthrene in the leaves of the control group is 63%, and the root is 89%.

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Abstract

The invention relates to the field of agricultural pollution restoration, in particular to a restoration method special for oryza sativa L. polluted by polycyclic aromatic hydrocarbon phenanthrene. The method specifically relates to application of phomopsis liquidambari HQ285146 in preparation of a polycyclic aromatic hydrocarbon phenanthrene degradation restoration agent special for oryza sativa L. The phomopsis liquidambari HQ285146 is activated and cultivated on a fluid nutrient medium or a solid medium containing potato dipping juice to obtain the oryza sativa L. restoration agent. Afterwards, the restoration agent is used for seedling dipping and is directly added into rhizosphere soil during transplant of oryza sativa L., and phenanthrene inside / outside oryza sativa L. bodies is effectively removed. Production of the agricultural phenanthrene pollution restoration agent is not affected by environment, the production period is short, the production process is simple, production time and yield are easy to control, and the effect is obvious. The method of plant dipping and direct agent adding into soil is adopted, the agent can act on polluted soil and plants, the method is simple and easy to operate, dosage is small, the cost is low, and the good effect can be achieved.

Description

technical field [0001] The invention relates to the field of agricultural pollution remediation, in particular to a method for in vivo and in vitro remediation of phenanthrene-contaminated rice by using plant endophytes. Background technique [0002] Polycyclic aromatic hydrocarbons (Polycyclic Aromatic Hydrocarbons, PAHs) refer to a class of condensed ring compounds containing two or more benzene rings arranged in a linear, angular or clustered form, and are an organic pollutant widely present in the environment. , Biological experiments show that PAHs are teratogenic, carcinogenic and mutagenic. In the past hundred years, due to industrial development and massive exploitation and utilization of fossil raw materials, the content of PAHs in the environment has increased year by year, greatly exceeding the self-purification capacity of the ecosystem. However, PAHs have poor water solubility and high octanol partition coefficient. They are often adsorbed on soil particles, ab...

Claims

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Application Information

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IPC IPC(8): B09C1/10C12N1/14C12R1/645
CPCC12N1/14B09C1/105C12R2001/645C12N1/145
Inventor 贾永戴传超傅婉秋
Owner NANJING NORMAL UNIVERSITY
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