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Plant endophytic fungus phomopsis D2G7 and application thereof

A technology of plant endophytic fungi, Phomopsis, applied in plant growth regulators, plant growth regulators, applications, etc., can solve problems such as physical health threats, hindering plant root respiration, and affecting plant growth and development

Active Publication Date: 2021-01-29
云南省生态环境科学研究院
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

The increase of heavy metal content in the soil will lead to changes in the original soil microbial flora structure and basic physical and chemical properties, and will also hinder the respiration of plant roots, thereby affecting the growth and development of plants, making it impossible for agricultural applications in areas polluted by heavy metals. Generally, the heavy metals in the soil can only be reflected when the crops enter the food chain and accumulate to a certain extent, which will lead to higher and higher concentrations of heavy metals in the soil
At the same time, heavy metals in the soil will enter the surface and groundwater through infiltration and leaching, causing water pollution, causing significant toxicity to plants, and then producing toxicity amplification through the food chain, posing a serious threat to human health.

Method used

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  • Plant endophytic fungus phomopsis D2G7 and application thereof
  • Plant endophytic fungus phomopsis D2G7 and application thereof
  • Plant endophytic fungus phomopsis D2G7 and application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0029] Example 1: Isolation, screening and identification of Phomopsis D2G7

[0030] A. Collect the plant samples of Nepeta nepeta, the dominant plant in the slag area of ​​the heavy metal polluted land, and rinse them under tap water;

[0031] B. Divide the plant sample into three parts: roots, stems and leaves, and carry out surface disinfection respectively. First, soak in ethanol solution with a volume concentration of 75% for 3 to 5 minutes, rinse with sterile water for 3 to 5 times, and then use available chlorine with a concentration of 5%. Soak in sodium hypochlorite solution for 2 to 3 minutes, rinse with sterile water for 3 to 5 times, and place it on sterile filter paper to absorb the water after rinsing; cut the root, stem, and leaf tissue pieces into fragments and paste them on a tissue containing 0.5g / Cultivate on the PDA medium of L streptomycin sulfate and 0.5g / L penicillin, culture at 24-26°C for 40-50 days, observe the next day, pick if there are colonies gr...

Embodiment 2

[0038] Example 2: Research on the Effect of Phomopsis D2G7 on the Accumulation of Low Concentration Heavy Metals

[0039] This example aims to prove that the filamentous fungus D2G7 provided by the present invention promotes plant growth and plant heavy metal pollution restoration in heavy metal pollution; Dysphania ambrosioides ) is the test plant, and the experimental process is as follows:

[0040] A. Preparation of sterile seedlings of Nepeta nepeta: The seed samples of Nepeta nepeta were collected on August 10, 2018 at the abandoned slag pile next to Sanduoduo Village, Zhehai Town, Huize County (26°28′17″ north latitude, 103 east longitude °37′34″, 2273 m above sea level), stored at 4°C for later use, randomly selected a few nepeta seeds from soil, and carried out surface disinfection according to the following procedures: first soak in 75% ethanol solution for 3 minutes, rinse with sterile water for 4 Then soak in NaClO solution with an effective chlorine concentration...

Embodiment 3

[0056] Example 3: Effects of Phomopsis D2G7 on the growth of Nepeta nepetae under low-concentration heavy metal stress

[0057] Experimental process and technical method are the same as embodiment 2, and difference is during the growth period of Nepeta nepeta, increased the mensuration of nepeta seedling biomass; Transplanting nepeta nepeta seedlings harvested the 46th day, measured strain Height, root length, dry weight, MDA, chlorophyll, glutathione GSH, etc.; GSH=T-GSH-2GSSG to calculate GSH content;

[0058] The results of the pot experiment figure 2 , 3 , 4 showed that the treatment group inoculated with Phomopsis D2G7 increased the root length, dry weight and chlorophyll content of Nepeta nepeta by 6.02%, 4.34% and 4.37%, respectively. However, the plant height, T-GSH, GSH and GSSG contents of the treatment group decreased by 9.93%, 38.41%, 52.48% and 14.71%, respectively. Among them, plant height, chlorophyll, T-GSH and GSH had significant differences between the ex...

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Abstract

The invention discloses a plant endophytic fungus phomopsis (Phomopsis columnaris) D2G7. The preservation number of the plant endophytic fungus phomopsis cloumnaris D2G7 in the China General Microbiological Culture Collection Center is CGMCC No. 21044. The strain is applied to bioremediation of heavy metals lead, zinc and cadmium; under the stress of heavy metal pollution, the strain can promote plant growth, promote the transfer capacity of plants to heavy metals, has strong accumulation capacity to heavy metals cadmium and zinc, and is suitable for in-situ remediation of large-area heavy metal cadmium and zinc pollution.

Description

technical field [0001] The invention belongs to the field of environmental management, and in particular relates to a plant endophytic fungus Phomopsis ( Phomopsis columnis ) D2G7 and its application in bioremediation of heavy metal lead, zinc, cadmium pollution. Background technique [0002] Environmental heavy metal pollution mainly refers to the pollution caused by the density greater than 5g / cm 3 And the environmental pollution caused by metals with atomic number above 24 or their compounds. According to statistics, the area of ​​cultivated land polluted by heavy metals in China is as high as 20 million hectares, accounting for more than 1 / 6 of the country's cultivated land area. Heavy metals are different from organic pollutants. Heavy metal molecules can be released into soil through interception, methylation reaction, redox reaction, chemical and biological transformation and other processes, and are retained in it through adsorption, precipitation and complexatio...

Claims

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Application Information

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IPC IPC(8): C12N1/14B09C1/10A01N63/30A01P21/00C12R1/645
CPCB09C1/105A01N63/30C12R2001/645C12N1/145
Inventor 和丽萍李海燕李丽娜李敏敏崔灿文江悦娟
Owner 云南省生态环境科学研究院
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