Method for preparing cytochalasin H by using endophytic fungi in mangrove forest

A technology of cytochalasin and endophytic fungi, applied in the field of preparation of cytochalasin H

Inactive Publication Date: 2016-09-07
GUANGDONG MEDICAL UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no industrial production method suitable for the production of cytochalasin H. Therefore, it is of great significance to develop a method that can realize the industrial production of cytochalasin H.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0058] S1. The endophytic fungus Phomopsis B3 ( Phomopsis liquidambari ) strains were inoculated into the sterilized culture medium; the culture medium contained raw materials in the following weight ratio: tryptone 100 g; soybean peptone 50 g; glucose 20 g; maltose 30 g; seawater 1000 g; The pH value is 7.0;

[0059] S2. Put it into the Erlenmeyer flask and seal the original tinfoil and kraft paper; shake and culture at 28°C for 7 days;

[0060] S3. After fermentation, filter with four layers of gauze to separate the fermentation broth and mycelium,

[0061] S4. Separation and purification of cytochalasin H (monomer) from the fermentation broth; the specific method of the separation and purification is prepared by preparative HPLC; the preparation conditions of the HPLC preparation are: use a preparative high performance liquid chromatograph , using a C18 chromatographic column; the mobile phase is acetonitrile and water, wherein the volume ratio of acetonitrile and water...

Embodiment 2

[0064] S1. The endophytic fungus Phomopsis B3 ( Phomopsis liquidambari ) strains were inoculated into the sterilized culture medium; the culture medium contained raw materials in the following weight ratio: tryptone 100 g; soybean peptone 50 g; glucose 20 g; maltose 30 g; seawater 1000 g; The pH value is 7.0; in addition, 2 mg porphyrin, 2 mg vitamin B7 and 6 mg fucosterol are added to the medium;

[0065] S2. Put it into the Erlenmeyer flask and seal the original tinfoil and kraft paper; shake and culture at 28°C for 7 days;

[0066] S3. After fermentation, filter with four layers of gauze to separate the fermentation broth and mycelium,

[0067] S4. Separation and purification of cytochalasin H (monomer) from the fermentation broth; the specific method of the separation and purification is prepared by preparative HPLC; the preparation conditions of the HPLC preparation are: use a preparative high performance liquid chromatograph , using a C18 chromatographic column; the ...

Embodiment 3

[0070] S1. The endophytic fungus Phomopsis B3 ( Phomopsis liquidambari ) strains were inoculated into the sterilized culture medium; the culture medium contained raw materials in the following weight ratio: tryptone 100 g; soybean peptone 50 g; glucose 20 g; maltose 30 g; seawater 1000 g; The pH value is 7.0; in addition, 2 mg porphyrin, 2 mg vitamin B7 and 2 mg fucosterol are added to the medium;

[0071] S2. Put it into the Erlenmeyer flask and seal the original tinfoil and kraft paper; shake and culture at 28°C for 7 days;

[0072] S3. After fermentation, filter with four layers of gauze to separate the fermentation broth and mycelium,

[0073] S4. Cytochalasin H (monomer) is obtained by separating and purifying from the fermentation broth; the specific method of said separation and purification is: put the macroporous adsorption resin on the fermentation broth, first wash it with water to remove impurities, and then use a volume fraction of 50% ethanol elution, collect...

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Abstract

The invention relates to the technical field of medicinal chemistry and particularly discloses a method for preparing cytochalasin H by using endophytic fungi in a mangrove forest. The preparation method includes the following steps that S1, phomopsis liquidambari B3 strains of the endophytic fungi in the mangrove forest are inoculated to a sterilized culture medium; S2, culture is performed under the condition of the temperature of 25-35 DEG C for 5-30 days; S3, fermentation liquid and mycelium are separated; S4, separation and purification are conducted on the fermentation liquid and/or the mycelium to obtain the cytochalasin H. The preparation method is simple and convenient, and the prepared cytochalasin H is high in content and is suitable for industrialized production.

Description

[0001] technical field [0002] The invention relates to the technical field of medicinal chemistry, in particular to a method for preparing cytochalasin H from mangrove endophytic fungi. Background technique [0003] Cytochalasin is a metabolite of a group of fungi, which can cut off the microfilament after binding to the microfilament in the cell, and bind to the end of the microfilament to inhibit the polymerization of actin at this site, but it has no effect on the detachment of the microfilament. Polymerization has no obvious effect, so cells treated with cytochalasin can destroy the grid structure of microfilaments and prevent the movement of cells, which is a specific drug that affects the assembly of microfilaments. Cytochalasin H (cytochalasin H) is a kind of cytochalasin. Studies have shown that it has various significant activities such as anti-tumor, anti-oxidation, and anti-angiogenesis. At present, there is no industrial production method suitable for the pro...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/02C07K1/16C12R1/645
CPCC07K14/37
Inventor 唐旭东巫鑫刘欣马越凡丰晓威
Owner GUANGDONG MEDICAL UNIV
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