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Biocontrol strain LS01 for preventing and controlling phomopasis asparagi and biocontrol inoculant of biocontrol strain

A technology for asparagus stem blight and biocontrol fungicides, which is applied in the directions of biocides, fungicides, biocides, etc., can solve the problems of high pesticide residues, outstanding chemical pesticide resistance, and high cost, and achieves good control effects without difficulty. The effect of drug resistance and non-pollution of the environment

Active Publication Date: 2017-06-23
CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Aiming at the current asparagus planting process of stem blight prevention and control is difficult, high cost, increasingly prominent resistance to chemical pesticides and high pesticide residues, the present invention provides a Paenibacillus polymyxa strain LS01, the preservation of the strain The number is CGMCC NO. 11690, and the preservation date is November 20, 2015. The preservation unit is the General Microbiology Center of the China Microbiological Culture Collection Management Committee, and the preservation address is No. 3, No. 1, Beichen West Road, Chaoyang District, Beijing

Method used

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  • Biocontrol strain LS01 for preventing and controlling phomopasis asparagi and biocontrol inoculant of biocontrol strain
  • Biocontrol strain LS01 for preventing and controlling phomopasis asparagi and biocontrol inoculant of biocontrol strain
  • Biocontrol strain LS01 for preventing and controlling phomopasis asparagi and biocontrol inoculant of biocontrol strain

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0011] Example 1 Paenibacillus polymyxa LS01 biocontrol agent and its preparation method.

[0012] In this example, Paenibacillus polymyxa LS01 (Paenibacillus polymyxa CGMCC NO. 11690) is used to make a biocontrol agent. Obtained after the bacterial agent is prepared, the specific preparation method comprises the following steps:

[0013] (1) Activation: Inoculate Paenibacillus polymyxa LS01 onto LB solid medium at a culture temperature of 37°C for 3-4 days; then transfer to LB liquid medium at a temperature of 30°C and shake at 180 rpm Cultivate for 16-28h.

[0014] (2) Seed culture: transfer the activated bacterial solution in step (1) into a 250 mL Erlenmeyer flask, at 30°C, 180 rpm, and vibrate for 16-28 hours until the logarithmic growth phase;

[0015] (3) Production culture: inoculate the bacterial liquid obtained in step (2) into the fermenter at an inoculation amount of 0.5%-2% of the total volume of the LB production medium, the tank temperature is 30°C, and the st...

Embodiment 2

[0017] Example 2 The inhibitory effect of Paenibacillus polymyxa LS01 biocontrol agent on Phomopsis asparagus.

[0018] Phomopsis, the pathogen of asparagus stem blight, has been activated twice, add 10mL of sterile water, carefully scrape off the spores and hyphae with an inoculation needle, then pour it into a triangular flask with glass beads, and shake it on a shaker Break up the bacteria block and let it stand still. Pour the supernatant into 300mL PDA medium that has been cooled to 45°C. After shaking well, pour it onto the plate immediately. After cooling and solidifying, place sterile small round filter paper (2 pieces / dish) , and cultured overnight at 30°C. Dilute the Paenibacillus polymyxa LS01 biocontrol agent and sterile water at a ratio of 1:20, then take 3 μL dropwise on the filter paper of the pathogen culture medium, use sterile water as the blank control group, and incubate at 30°C for 24-36 h , observe the inhibition zone.

[0019] The results of the antiba...

Embodiment 3

[0022] Example 3 Greenhouse pot test to detect the control effect of Paenibacillus polymyxa LS01 biocontrol agent on asparagus stem blight

[0023] Before transplanting, dilute the asparagus seedlings with Paenibacillus polymyxa LS01 biocontrol agent 100 times, soak the seeds for 30 minutes; use 20 mL biocontrol agent dilution to treat the roots when transplanting, and inoculate 1000 asparagus seedlings 7 days after transplanting. / mL Phomopsis asparagus zoospore suspension 20mL. In addition, the treatment of directly inoculating the zoospore suspension of Phomopsis asparagus without Paenibacillus polymyxa LS01 biocontrol agent was used as the control group. Each treatment group consisted of 20 plants, repeated three times. At a temperature of 25-28°C and a relative humidity of 50%, the disease progression was investigated after 4 weeks of cultivation, and the disease severity and control effect were calculated.

[0024] The grading standard of root irrigation and inoculatio...

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Abstract

The invention relates to a biocontrol strain LS01 for preventing and controlling phomopasis asparagi and an application of the biocontrol strain to a biocontrol inoculant, and belongs to the technical field of plant protection. The invention provides the paenibacillus polymyxa LS01 and the application thereof as well as the biocontrol inoculant comprising the strain (the paenibacillus polymyxa) and an application of the biocontrol inoculant. The biocontrol strain LS01 provided by the invention is the paenibacillus polymyxa, which is preserved in China General Microbiological Culture Collection Center, with strain preservation number of CGMCC NO. 11690. The biocontrol inoculant is prepared by conducting shaking culture on the strain in an LB culture solution at 28-37 DEG C at 180rpm for 20-48h, implementing centrifuging, collecting bacteria and processing the bacteria by virtue of a microbial freeze-drying method, wherein the total concentration of living bacteria in the finished inoculant product ranges from 1*10<6>CFU / g to 1*10<11>CFU / g; and in practical application, the finished inoculant product is applied in the form of a bacterium suspension which is obtained by diluting the finished inoculant product with sterile water at the ratio of 1: 20. Based upon tests, the biocontrol inoculant provided by the invention can achieve a bacteriostasis rate on asparagus phomopsis to 100%, and under a room-temperature condition (artificial inoculation), an effect of preventing the phomopasis asparagi can reach 76.07%.

Description

technical field [0001] The invention relates to a biocontrol bacterial strain LS01 for preventing and treating asparagus stem blight and an application thereof, belonging to the technical field of plant protection, and in particular to the prevention and treatment of asparagus stem blight. Background technique [0002] Stem blight of asparagus is a devastating disease with wide distribution and serious damage, which occurs in almost every asparagus producing area in my country. In recent years, the area of ​​asparagus in my country has continued to expand, and its harm has also increased. Once got stem blight, light then reduces yield, and heavy then destroys garden and has no harvest, causes huge loss to numerous bamboo shoot growers. At the same time, asparagus stem blight is called "asparagus cancer" because it is difficult to eradicate once infected. [0003] Chemical pesticides are usually used to control asparagus stem blight, although some control effects have bee...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A01N63/00A01N63/02A01P3/00C12R1/01
CPCA01N63/00A01N63/10C12N1/205C12R2001/01
Inventor 程池刘洋陈建国梁寒峭尹俊玉李生有李国栋
Owner CHINA NAT RES INST OF FOOD & FERMENTATION IND CO LTD
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