15 results about "Acetic acid concentration" patented technology

The invention relates to the technical field of silage, in particular to a whole-plant corn silage additive and application thereof. The whole-plant corn silage additive is prepared from bacillus subtilis GW-910 and bacillus subtilis GW-908. The whole corn silage is carried out by adding the bacillus with lignocellulose degradation capability as the silage additive, so that the concentrations of lactic acid and acetic acid are remarkably increased, the concentration of butyric acid is reduced, the contents of NDF, ADF, ADL and starch are remarkably reduced, the content of WSC is remarkably increased, and the quality of the whole corn silage is remarkably improved.

The invention discloses an acetate-resistant domesticated algal strain HpAc50 and an application thereof. The algal strain is preserved in the China Center for Type Culture Collection on January 12, 2026, and the preservation number is CCTCC M 202690. The haematococcus pluvialis strain is obtained by carrying out acetate-resistant domestication on haematococcus pluvialis and can be cultured under the condition that the acetic acid concentration is 0-10g / L, and the yield of astaxanthin is effectively increased. The invention specifically provides three application conditions: illumination of 0-5000lux and a full-nutrient culture medium; illumination is performed at 0-5000 lux, a nitrogen-phosphorus-deficient culture medium is adopted, and an exogenous stimulant is added According to the present invention, with the application of the strain, the cell density, the biomass and the astaxanthin accumulation can be significantly improved under the conditions of illumination of 5000-15000 lux and nitrogen and phosphorus deficiency culture medium, such that the strain has strong acetate tolerance and strong production performance compared with the wild type strain, and is suitable for the large-scale astaxanthin production.

The invention discloses a guanidinoacetic acid N-methyltransferase mutant and application of the guanidinoacetic acid N-methyltransferase mutant in synthesis of creatine, and belongs to the technical field of bioengineering. The invention provides a method for preparing creatine by utilizing a guanidinoacetic acid N-methyltransferase mutant (Y134I / N170H) and catalyzing guanidinoacetic acid and S-adenosylmethionine in a whole-cell form. The genetically engineered bacterium for expressing the guanidinoacetic acid N-methyltransferase mutant is fermented for 24 hours under the conditions that the whole cell amount is 0.2 g, the pH value is 7.5, the reaction temperature is 37 DEG C, the concentration of guanidinoacetic acid is 80 mM, and the concentration of S-adenosylmethionine is 100 mM, the creatine yield is 7.34 g / L, and the molar yield is 69.96%. According to the method disclosed by the invention, the production cycle is shortened, the yield of creatine is increased, and the industrial process of producing creatine by an enzyme conversion method is accelerated.

The invention discloses an optimal control method and system for agricultural waste compost, a medium and a product, and the method comprises the steps: monitoring temperature data, ethanol concentration data, acetic acid concentration data and pH values of a plurality of preset detection regions in a target compost body; calculating a first risk index of each preset detection area; determining a first regulation and control parameter of the first preset detection area based on the first risk index; controlling a ventilation module to ventilate the first preset detection area based on the first regulation and control parameter; determining an acetification potential index of each first preset detection area; calculating a second risk index of each first preset detection area based on the acetification potential index; determining a second regulation and control parameter of the second preset detection area based on the second risk index; and controlling the pH value regulation and control module to carry out pH value regulation and control on the second preset detection area based on the second regulation and control parameter. The agricultural waste composting efficiency can be improved.

The invention discloses a method for detecting the concentration of acetic acid in an anaerobic fermentation system by using a bioelectrochemical sensor, acetic acid is detected by using the bioelectrochemical sensor, the bioelectrochemical sensor comprises a screen-printed electrode, and the working surface of the screen-printed electrode is modified with reduced graphene oxide and polymethylene blue. The working surface of the screen printing electrode is connected with acetokinase, pyruvate kinase and lactic dehydrogenase through a cross-linking agent. According to the method, the adopted sensor has better conductivity, higher response speed and higher current intensity, when the sensor is used for detecting the concentration of acetic acid in a fermentation system, the sensor has the advantages of being easy to operate, low in sample consumption, low in price, high in accurate measurement precision, high in detection speed and the like, and rapid detection of the concentration of acetic acid can be achieved; the acetic acid concentration in the anaerobic fermentation process in different periods can be timely and accurately obtained, effective data can be provided for anaerobic fermentation analysis, and the method has important significance for monitoring the process stability of anaerobic fermentation.

The invention discloses a continuous gas fermentation regulation and control method, and solves the technical problem of low ethanol yield of a fermentation system in the prior art under the condition of fluctuation of industrial tail gas. The regulation and control method comprises the following steps: acquiring the current acetic acid concentration, ethanol concentration, carbon monoxide demand and carbon monoxide supply of the fermentation system; when the fluctuation values of the acetic acid concentration and the ethanol concentration are within a set range and the carbon monoxide supply amount is greater than the carbon monoxide demand amount, increasing the process parameters of the fermentation system in multiple stages until the dilution rate reaches a first set value; when the fluctuation values of the acetic acid concentration and the ethanol concentration are within the set range and the carbon monoxide supply amount is smaller than the carbon monoxide demand amount, the dilution rate of the fermentation system is lowered in multiple stages until the dilution rate reaches a second set value, and the first set value is larger than the second set value. The continuous gas fermentation regulation and control method provided by the invention improves the yield of ethanol.

This invention discloses a culture medium for the biosynthesis of indigoidine and its application, belonging to the field of microbial fermentation and synthetic biology technology. Addressing the problems of low yield, insufficient precursor supply, byproduct accumulation, and poor scale-up stability in existing culture media for indigoidine fermentation, this invention provides a core culture medium system. This medium, per 1L, includes: 10-50g carbon source, 5-20g nitrogen source, 10-30g L-glutamine, and 0.1-20g inorganic salts, with optional addition of 0.1-20mL trace element salt solution and / or vitamin components. The pH is 6.0-7.5. The carbon source is selected from glucose, glycerol, sucrose, dextrin, or combinations thereof; the nitrogen source is selected from ammonium sulfate, yeast extract, peptone, or combinations thereof. This culture medium can significantly increase the yield of indigoidine synthesized by recombinant *E. coli* and effectively inhibit acetic acid accumulation. In a 5L fermenter, compared with LB medium, this medium can increase the indigoidine yield by at least 80% while reducing the acetic acid concentration in the fermentation broth by more than 60%, showing good prospects for industrial application.

The invention discloses a self-flocculation fermentation lactobacillus mucus LC01 with high lactic acid yield, the preservation number of the self-flocculation fermentation lactobacillus mucus LC01 is CGMCC (China General Microbiological Culture Collection Center) No.32545, and the self-flocculation fermentation lactobacillus mucus LC01 has the capability of efficiently producing lactic acid, acetic acid and other short-chain fatty acids, has better tolerance to low pH (Potential of Hydrogen), high pressure, oxygen stress and the like, and has rapid self-flocculation capability. The invention also discloses an application of the fermented lactobacillus mucus in short-chain fatty acid production, the fermentation conditions are as follows: the temperature is 37 DEG C, the speed is 200rpm, the final lactic acid concentration is 6.88 mg / mL, and the final acetic acid concentration is 4.18 mg / mL; and rapid separation of thalli and fermentation liquor is realized by utilizing the self-flocculation capability of the strain at the fermentation end point.

A method for synthesizing caproic acid by carbon chain extension based on two-stage anaerobic fermentation is provided. The method includes the following steps: performing alkali pretreatment on concentrated sludge; performing first anaerobic fermentation on the concentrated sludge subjected to alkali pretreatment to obtain a fermentation product; and mixing the fermentation product, caproic acid-producing bacteria, an electron donor, nutrients and a first methane inhibitor, and performing second anaerobic fermentation to obtain the caproic acid. Different from a one-stage anaerobic fermentation system that features a slow reaction process due to the lack of short-chain fatty acids, the two-stage anaerobic fermentation process generates more caproic acid. In addition, the alkali pretreatment effectively destroys the structure of extracellular polymeric substances, which can release more dissolved organic matter, increase the concentration of acetic acid, provide more organic substrate, and improve the synthesis of caproic acid.

The application discloses a kind of liquid ring vacuum pump working liquid for acetic anhydride production improvement and material recycling process, belong to acetic anhydride production technical field.The application is aimed at the defects of working liquid pollution, low temperature freeze block, material waste and big environmental protection pressure of existing liquid ring vacuum pump with water as working liquid, replace working liquid with acetic anhydride, carry out material quality adaptation improvement to liquid ring vacuum pump, and make acetic anhydride occur controllable hydrolysis reaction by pre-treatment dehydration, moisture isolation and supporting temperature control system, form acetic anhydride-acetic acid mixed solution, when acetic acid concentration reaches 40%-50%, mixed solution is directly recycled for acetic anhydride production process without separation and purification.The application solves the problems of working liquid pollution and low temperature freeze block, realizes material resource recycling, improves material utilization, reduces production cost and environmental protection pressure, equipment runs stably, has long service life, process is simple, easy industrialization popularization, and is suitable for vacuum system optimization of various acetic anhydride production devices.

The application discloses a high-stability cerium dioxide polishing liquid based on an acetic acid-ammonium acetate buffer system.The polishing liquid comprises the following components: nanometer cerium dioxide with a concentration of 1.0wt%-3.0wt%; acetic acid with a concentration of 0.1wt%-0.5wt%, ammonium acetate with a concentration of 0.05wt%-0.1wt%, and the rest is water; and the pH value is 3.5-5.0.The dispersion performance, polishing rate and stability of the polishing liquid are obviously improved, and the quality and performance of the polishing liquid are greatly improved.

The invention discloses a pretreatment mode for making chicken skin crisp and tender, and belongs to the technical field of food processing treatment.The pretreatment mode for making the chicken skin crisp and tender comprises the following specific steps that firstly, the chicken skin is placed in an alkaline treatment tank, and the chicken skin is soaked in an alkaline solution to be subjected to permeation treatment; 2, transferring the chicken skin treated in the step 1 into an acid solution in a low-temperature acid treatment cabin for low-temperature tightening treatment; and 3, putting the chicken skin treated in the step 2 into an ultrasonic enzymolysis tank, and carrying out ultrasonic-assisted compound enzyme treatment. Through the steps of alkali, acid, enzyme and gradient dehydration, the crispness of the chicken skin is greatly improved, the tenderness is remarkably improved, a uniform crispy layer is formed, compared with traditional frying, the oil absorption amount is reduced, the treatment liquid can be recycled for multiple times, the raw material cost is greatly reduced, meanwhile, a strong corrosion reagent is avoided, and the concentration of residual acetic acid meets the food safety standard.

The invention provides a PQQ-based acetic fermentation process control method, which comprises the following steps: firstly, carrying out acetic fermentation to obtain a fermented beverage; measuring the PQQ content Vp and the acetic acid content V1 in the fermented beverage, taking the PQQ content Vp and the acetic acid content V1 as acetic acid fermentation markers, obtaining a fermentation index m according to the Vp and the V1, and obtaining an enzyme activity factor K according to the m; finally, the fermentation degree is judged according to the size of the enzyme activity factor K, when K is larger than 50%, it is judged that fermentation is over-fire, fermentation parameters are adjusted, and the step S1 is executed again for acetic fermentation; when K is smaller than 25%, fermentation is insufficient, fermentation parameters are adjusted, and acetic fermentation is conducted again; when K is larger than or equal to 25% and smaller than or equal to 50%, the fermentation degree is appropriate, and the acetic acid fermentation process control method based on PQQ is completed. The fermentation coenzyme and the acetic acid concentration are used as control parameters of the fermentation process, so that the control difficulty of the acetic acid fermentation process is greatly reduced, and the control efficiency is improved.

Examples relate to systems and methods for concentrating acetic acid solutions using multi-tier ultrahigh pressure (“UHP”) reverse osmosis. Along with an acetic acid product having a concentration of at least 25 weight percent (wt %), the systems and methods disclosed herein simultaneously provide water with an acetic acid concentration of 2 wt % or less in at little as three tiers.

The invention relates to a method for producing ethyl acetate by using acetic acid in fermentation wastewater, which comprises the following steps: carrying out on-line acetic acid concentration monitoring on clostridium ethanolicum fermentation wastewater to obtain acetic acid-containing wastewater; dissolving a polymer in the buffer solution to obtain a polymer solution; the polymer solution and the CALB solution are sequentially subjected to stirring, microsphere forming and cross-linking curing, and immobilized CALB is obtained; putting the immobilized CALB, the acetic acid-containing wastewater, ethanol and an organic extraction agent into a reaction tank, and carrying out esterification reaction and real-time extraction to obtain a reaction mixture; pumping an organic extraction phase containing ethyl acetate in the upper layer of the reaction mixture into a rectifying tower for distillation separation to obtain ethyl acetate, and returning the organic extraction agent to the reaction tank for recycling; introducing the lower layer of the reaction mixture into a standing tank for phase separation to obtain a separated phase; pumping an organic extraction phase containing ethyl acetate at the upper layer of the separation phase into a reaction tank for recycling, and feeding a water phase at the lower layer of the separation phase into sewage treatment. Finally, resource utilization of the fermentation wastewater is realized.