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Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furfural and application thereof

A technology of Zymomonas and movement, which is applied in the field of microorganisms, can solve the problem that it is difficult to tolerate high concentrations of acetic acid and high concentrations of furan formaldehyde at the same time, and achieve the effect of saving detoxification treatment steps

Active Publication Date: 2015-11-18
BIOGAS SCI RES INST MIN OF AGRI
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Problems solved by technology

[0005] In order to overcome the defect that the existing Zymomonas mobilis is difficult to simultaneously tolerate high concentrations of acetic acid and high concentrations of furfuraldehyde, the present invention provides a strain of motile fermentation that can simultaneously tolerate up to 7g / L acetic acid and 3g / L furfuraldehyde Monascus strain

Method used

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  • Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furfural and application thereof

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preparation Embodiment 1

[0026] This preparation example is used to illustrate the preparation of RM medium containing acetic acid and furan formaldehyde, and the process of preparing an enzymatic hydrolyzate from a cellulose-containing raw material (corn stalk).

[0027] According to 20.0g / L glucose, 10.0g / L yeast powder, 1.0g / LMgCl 2 , 1.0g / L (NH 4 ) 2 SO 4 and 1.0g / LKH 2 PO 4 The formulation configuration of the base RM medium was obtained. Add acetic acid and furfuralaldehyde to the basic RM medium to obtain 3 kinds of RM adjusted medium, wherein, the first RM adjusted medium contains 6.0g / L acetic acid and 2.0g / L furfuralaldehyde, and the second RM adjusted medium Containing 7.0 g / L of acetic acid and 3.0 g / L of furfuraldehyde, the third RM conditioned medium contained 7.5 g / L of acetic acid and 3.5 g / L of furfuraldehyde.

[0028] Cut the corn stalks into small pieces of about 2 cm, then add water to adjust the water content to 40% by weight; transport them to the heat preservation pressure...

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Abstract

The invention provides zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furfural and application thereof. The accession number of the zymomonas mobilis is CMGCC No.10946. The zymomonas mobilis can quick grow in a culture solution containing 7.0 g / L of acetic acid and 3.0 g / L of furfural, and alcoholic fermentation can be performed quickly. Therefore, the zymomonas mobilis can directly perform fermentation on cellulose enzymatic hydrolysates on the conditions of high-concentration acetic acid and high-concentration furfural, and fuel alcohol is obtained through preparation.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furan formaldehyde and its application. Background technique [0002] Using my country's rich straw resources as raw materials to produce fuel ethanol and other bio-based products is of great significance to promoting the sustainable development of my country's agriculture, expanding agricultural functions and ensuring national energy security. However, in the process of fermenting lignocellulosic biomass resources such as straw to produce ethanol and bio-based chemicals, due to the dense structure between cellulose, hemicellulose and lignin in the raw materials, it is not conducive to the subsequent enzymatic saccharification of cellulose . Therefore, before enzymatic hydrolysis, lignocellulosic biomass generally needs to undergo physical and chemical pretreatment methods to improve th...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20C12P7/10C12P19/14C12R1/01
CPCY02E50/10
Inventor 何明雄税宗霞王景丽祝其丽秦晗张敏吴波谭芙蓉代立春
Owner BIOGAS SCI RES INST MIN OF AGRI
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