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A kind of Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furfural formaldehyde and its application

A technology of Zymomonas and exercise, applied in the field of microorganisms, can solve the problem of being difficult to tolerate high concentrations of acetic acid and high concentrations of furan formaldehyde at the same time, and achieve the effect of saving detoxification treatment steps.

Active Publication Date: 2018-07-13
BIOGAS SCI RES INST MIN OF AGRI
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Problems solved by technology

[0005] In order to overcome the defect that the existing Zymomonas mobilis is difficult to simultaneously tolerate high concentrations of acetic acid and high concentrations of furfuraldehyde, the present invention provides a strain of motile fermentation that can simultaneously tolerate up to 7g / L acetic acid and 3g / L furfuraldehyde Monascus strain

Method used

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  • A kind of Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furfural formaldehyde and its application

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preparation Embodiment 1

[0026] This preparation example is used to illustrate the preparation of RM medium containing acetic acid and furan formaldehyde, and the process of preparing an enzymatic hydrolyzate from cellulose-containing raw materials (corn stalks).

[0027] According to 20.0g / L glucose, 10.0g / L yeast powder, 1.0g / L MgCl 2 , 1.0g / L (NH 4 ) 2 SO 4 and 1.0g / LKH 2 PO 4 The formulation configuration of the base RM medium was obtained. Acetic acid and furfuraldehyde were added to the basic RM medium to obtain 3 kinds of RM adjusted medium, wherein, the first RM adjusted medium contained 6.0g / L acetic acid and 2.0g / L furfuraldehyde, and the second RM adjusted medium Containing 7.0 g / L of acetic acid and 3.0 g / L of furfuraldehyde, the third RM conditioned medium contained 7.5 g / L of acetic acid and 3.5 g / L of furfuraldehyde.

[0028] Cut the corn stalks into small pieces of about 2 cm, then add water to adjust the water content to 40% by weight; transport them to the heat preservation pre...

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Abstract

The invention provides a Zymomonas mobilis resistant to high concentration of acetic acid and high concentration of furfuraldehyde, and the preservation number of the Zymomonas mobilis is CGMCC No.10946. The Zymomonas mobilis of the invention can grow rapidly in the culture solution containing more than 7.0 g / L of acetic acid and more than 3.0 g / L of furfuraldehyde, and can rapidly carry out ethanol fermentation. This enables the Zymomonas mobilis of the present invention to directly ferment the cellulose hydrolysis product under the conditions of higher acetic acid concentration and higher furan formaldehyde concentration, and prepare fuel ethanol.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to a Zymomonas mobilis resistant to high-concentration acetic acid and high-concentration furan formaldehyde and its application. Background technique [0002] Using my country's rich straw resources as raw materials to produce fuel ethanol and other bio-based products is of great significance to promoting the sustainable development of my country's agriculture, expanding agricultural functions and ensuring national energy security. However, in the process of fermenting lignocellulosic biomass resources such as straw to produce ethanol and bio-based chemicals, due to the dense structure between cellulose, hemicellulose and lignin in the raw materials, it is not conducive to the subsequent enzymatic saccharification of cellulose . Therefore, before enzymatic hydrolysis, lignocellulosic biomass generally needs to undergo physical and chemical pretreatment methods to improve th...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P7/10C12P19/14C12R1/01
CPCY02E50/10
Inventor 何明雄税宗霞王景丽祝其丽秦晗张敏吴波谭芙蓉代立春
Owner BIOGAS SCI RES INST MIN OF AGRI
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