L-tryptophane fermentation process

A fermentation process and tryptophan technology, which is applied in the field of L-tryptophan fermentation process, can solve the problems of excessive accumulation of acetic acid and inhibit fermentation acid production, so as to prolong the acid production cycle, improve the sugar-acid conversion rate and the filtration rate. improved effect

Inactive Publication Date: 2016-11-09
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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Problems solved by technology

[0008] The technical problem to be solved by the present invention is to provide a fermentation process of L-tryptophan, which effectively solves the p

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  • L-tryptophane fermentation process

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Embodiment 1

[0033] A fermentation process for L-tryptophan, the specific steps are as follows:

[0034] (1) such as figure 1 As shown, the filter device is assembled. The filter device includes a 30L fermentation tank 1, a gas-liquid separator 2, a variable-speed regulating pump 4, a membrane separation device 5, and a storage tank 6, and the fermentation tank outlet and the gas-liquid separator ( The material inlet of the cyclone gas-liquid separator can be connected, the gas outlet of the gas-liquid separator is connected to the high-temperature air filter membrane 3 through a long pipe, and the bottom of the gas-liquid separator is at an angle of 60° with the horizontal plane. The liquid outlet is located at the bottom of the cone, and the liquid outlet is connected to the membrane separation device through a variable speed regulating pump. The membrane separation device is respectively connected to the storage tank and the fermentation tank, and the membrane separation device is provided...

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Abstract

The invention provides an L-tryptophane fermentation process. The fermentation process comprises the following steps: adding 2g cottonseed cake powder to every 1 liter of an L-tryptophane fermentation medium to carry out fermentation; carrying out circulating filtration in the fermentation course when the yield of acetic acid is more than 5g/L and allowing clear fermentation liquid obtained after separation to enter into an extraction procedure; allowing concentrated bacteria liquid obtained after extraction to enter a fermentation tank, then replenishing a dialysis medium and then carrying out fermentation further, thus obtaining L-tryptophane. By adopting the fermentation process, the L-tryptophane production cycle is lengthened by 20 hours, the acetic acid concentration is maintained below 5g/L, the single tank yield is increased by 89% or above, and the conversion rate is increased from 17.0% to more than 18.5%; as the cottonseed cake powder is added to the fermentation medium and the cottonseed cake powder which is not utilized by thalli plays a role of a filter aid during filtration, the fermentation liquid filtering rate is increased from 85mL/min to 183mL/min.

Description

Technical field [0001] The invention relates to the field of tryptophan production, in particular to a fermentation process of L-tryptophan. Background technique [0002] L-tryptophan, also known as L-α-amino-β-indolylalanine, chemical name L-2-amino-3-indolylalanine, is a neutral aromatic amino acid containing indole groups It plays an important role in the metabolism of humans and animals. It is one of the eight essential amino acids in humans and animals. The physical properties of L-tryptophan are white or slightly yellow leaf-like crystals or powders, odorless or slightly odorous, sweet, soluble in hot pyridine, slightly soluble in ethanol and insoluble in chloroform and ether. A small amount of indole will be produced when L-tryptophan is heated with water, and a large amount of indole will be produced when heated in the presence of NaOH and CuSO4. At present, L-tryptophan is widely used in medicine, food and feed. [0003] In medicine, L-tryptophan is the precursor for th...

Claims

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Application Information

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IPC IPC(8): C12P13/22
CPCC12P13/227
Inventor 陈宁徐庆阳刘镇瑜谢希贤杨梦晨张成林李燕军范晓光
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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