A kind of vancomycin culture medium and the method for preparing vancomycin

A technology of vancomycin and culture medium, applied in the field of drug production, can solve problems such as changing yield and quality, and achieve the effects of enhancing safety and effectiveness, increasing yield, improving yield and purity

Active Publication Date: 2018-12-25
雅赛利(台州)制药有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the case of existing strains, the improvement of the yield and quality of vancomycin can only be changed by optimizing the process.

Method used

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  • A kind of vancomycin culture medium and the method for preparing vancomycin
  • A kind of vancomycin culture medium and the method for preparing vancomycin
  • A kind of vancomycin culture medium and the method for preparing vancomycin

Examples

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Effect test

Embodiment 6

[0039] The difference between the preparation method of the medium in Example 6 and the medium in Examples 1-5 is that no amino acid complex is added in step 2, and no pH regulator is added in step 3 to adjust the pH.

[0040] refer to figure 1 , embodiment 7-9 is the preparation of vancomycin

Embodiment 7

[0042] The medium prepared according to Example 1 was selected for use.

[0043] Step 1: seed inoculation in shake flasks, culture in vancomycin medium in seed tanks, culture in vancomycin medium in fermenters to obtain vancomycin fermentation broth. Place the vancomycin fermentation broth in a pretreatment tank, control the rotation speed to 80 rad / min, and the temperature is 25°C, add 0.1% of the volume of the fermentation broth with hydrochloric acid with a mass fraction of 10% to form a salt, and stir for 30 minutes to obtain a pretreatment liquid .

[0044] Step 2: Filtration with a ceramic membrane. A multi-channel ceramic membrane is selected, the number of channels is 10, the membrane material is silicon dioxide, and the pore size is 0.2 μm. The cross-flow mode is used for circulating filtration, and the pressure is controlled at 0.1MPa.

[0045] Step 3: After filtering through the ceramic membrane, enter the primary nanofiltration unit. The nanofiltration uses an ar...

Embodiment 8

[0064] The difference between embodiment 8 and embodiment 7 is:

[0065] The medium prepared according to Example 2 was selected for use.

[0066] In step 1, hydrochloric acid with a mass fraction of 12% of 0.5% of the volume of the fermented liquid is added for salification; in step 2, ceramic membranes are used for filtration, and multi-channel ceramic membranes are selected, and the number of channels is 8; in steps 3, 7 and 14, The pressure is controlled at 2MPa, the temperature of the filtrate is controlled at 21°C, the flow rate of the nanofiltration is controlled at 320L / h, and the concentration volume multiple of the nanofiltration is 4 times. After the nanofiltration is completed, use purified water for top washing for 8 minutes; After filtration, enter the oxalic acid precipitation tank, and then add 0.002% solid sodium oxalate of the concentrated solution obtained by nanofiltration once, and let it stand after stirring for 30 minutes; in steps 8 and 15, enter the pr...

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Abstract

The invention discloses a vancomycin culture medium and a method for preparing vancomycin. The vancomycin culture medium is prepared from, by weight, 1.5-5 parts of carbon source, 0.05-0.15 part of nitrogen source, 0.15-0.45 part of inorganic salt and 90-110 parts of water. The inorganic salt is prepared from nickel sulfate, ferric nitrate and sodium chloride according to the weight part ratio of (0.5-2):(0.5-2):1. The vancomycin culture medium and the method have the advantages that yield and purity of vancomycin are improved.

Description

technical field [0001] The invention relates to the field of medicine production, in particular to a vancomycin culture medium and a method for preparing vancomycin. Background technique [0002] In recent years, vancomycin has become the last line of defense against bacteria due to the large number of penicillin-resistant Gram-positive infections caused by the widespread use of penicillin. With the increasing demand for vancomycin, how to improve the existing vancomycin production process to increase the output of vancomycin has become a research hotspot. [0003] In the existing production process of vancomycin, the method of controlling the actinomycetes Amycolatopsis orientalis to produce vancomycin under fermentation conditions, and then obtaining pure vancomycin after separation and purification is generally adopted. It can be seen that the fermentation step of vancomycin can have a crucial impact on the yield in the whole production process. Therefore how to improve...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/20C12P21/02C12R1/01
CPCC12N1/20C12P21/005
Inventor 芦琦陈晓静
Owner 雅赛利(台州)制药有限公司
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