Method for extracting and isolating ruscogenin from pseudoleaf tree

A technology for separating ruscogenin and pseudophyllum, which is applied in the fields of steroids and organic chemistry, can solve the problems of long production cycle and low yield, achieve less usage, good selectivity, and less solvent consumption Effect

Active Publication Date: 2016-03-30
SHAANXI JIAHE PHYTOCHEM
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

This method also uses enzymatic hydrolysis of glycosides, which has defects such as low yield and long production cycle.

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0048] Take 100kg of false leaf tree raw material, add 50kg of water to pulverize, add 40kg of diatomaceous earth and 250kg of water after pulverization and stir evenly, add 60kg of glacial acetic acid to the above mixture system (make the mass fraction 20%), stir and hydrolyze at 90°C for 1 hour , filtered, and the precipitate was washed with water until pH=7. Add 10 times the amount of acetone to the precipitate, extract at 70°C for two hours, and filter. The filtrate is concentrated using membrane concentration equipment, the selected membrane has a molecular weight cut-off of 200-400, and is concentrated to 1 / 10 of the original volume to stop concentration. Put the concentrated solution in the refrigerator overnight to obtain white crystals. Add 8 times the amount of ethyl acetate to dissolve the white crystals, add 8% activated carbon, heat at 60°C for 1 hour, filter, concentrate to 1 / 3, place crystals in an ice bath, filter with suction, and dry in vacuo, white powder o...

Embodiment 2

[0050] Take 100kg of false leaf tree raw material, add 50kg of water to pulverize, add 12kg of alumina, 8kg of diatomaceous earth and 250kg of water after pulverization and stir evenly, add 45kg of sulfuric acid to the above mixture system (to make the mass fraction 15%), and stir at 90°C Hydrolyze for 2 hours, filter, and wash the precipitate with water to pH=5. Add 7 times the amount of chloroform to the precipitate, extract at 60°C for two hours, and filter. The filtrate is concentrated using membrane concentration equipment, the selected membrane has a molecular weight cut-off of 200-400, and is concentrated to 1 / 3 of the original volume to stop concentration. Put the concentrated solution in the refrigerator overnight to obtain white crystals. Add 4 times the amount of white crystals to 90% methanol to dissolve, add 5% activated carbon, heat at 50°C for 1 hour, filter, concentrate to 1 / 4, place crystals in an ice bath, filter with suction, and dry in vacuo, white powder ...

Embodiment 3

[0052] Take 100kg of false leaf tree raw material, add 50kg of water to pulverize, add 5kg of silica gel, 5kg of alumina and 250kg of water after pulverization and stir evenly, add 15kg of formic acid to the above mixture system (make the mass fraction 5%), stir and hydrolyze at 50°C for 5 hours, filtered, and washed the precipitate with water to pH=4. Add 5 times the amount of ethanol to the precipitate, extract at 80°C for two hours, and filter. The filtrate is concentrated by membrane concentration equipment, the selected membrane has a molecular weight cut-off of 400-600, and is concentrated to 1 / 6 of the original volume to stop concentration. Put the concentrated solution in the refrigerator overnight to obtain white crystals. Dissolve the white crystals in 5 times the amount of chloroform, add 5% activated carbon, heat at 40°C for 1 hour, filter, concentrate to 1 / 3, place crystals in an ice bath, filter with suction, and dry in vacuo to obtain a white powder of deluscog...

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PUM

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Abstract

The invention relates to a method for extracting and separating ruscogenin from Ruscus aculeatus, which comprises the following steps: 1) hydrolyzing the Ruscus aculeatus raw material to obtain a hydrolysis mixture; 2) filtering the hydrolysis mixture obtained in the step 1) and collecting sediment; 3) extracting the sediment obtained in the step 2) by using an organic solvent of 1-10 times of mass of the sediment and then collecting an extract; wherein the organic solvent is one or arbitrary combination of ethanol, ethyl acetate, n-butyl alcohol, chloroform, acetone and petroleum ether; 4) concentrating the extract obtained in the step 3) by using a membrane concentration device and collecting a concentrate; and 5) putting the concentrate obtained in the step 4) in a refrigerator overnight to obtain a coarse crystallization, recrystallizing the coarse crystallization to obtain white powder of ruscogenin. The invention provides the method for extracting and separating ruscogenin from Ruscus aculeatus with advantages of less application amount of organic solvent, time saving and less cost.

Description

technical field [0001] The invention belongs to the field of sapogenin extraction and separation, and relates to a method for extracting ruscogenin, in particular to a method for extracting ruscogenin from pseudophyllum. Background technique [0002] Ruscus aculeatus, a shrub belonging to the genus Ruscus aculeatus of the family Asparagaceae, likes warm, humid and sunny environments. Native to Eurasia, Western Europe and the Mediterranean coast. The branches and leaves of false leaves are dark green, and they are often cultivated as foliage plants. They are decorated in living rooms and halls. Another medical study has proved that the extract of the pseudoleaf tree can stimulate the production of vascular endothelial growth factor for the treatment of hair disorders, especially hair tufts and male pattern baldness. In addition, the extract of pseudoleaf tree can also be used in combination with other external drugs to induce the production of VEGF to prevent skin aging. A...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C07J71/00
Inventor 王晓莹肖红张瑜王春德
Owner SHAANXI JIAHE PHYTOCHEM
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