Threonine fermentation medium and clean production process of threonine
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A fermentation medium, clean production technology, applied in fermentation, microorganism-based methods, microorganisms, etc., can solve the problems of low threonine content in the extract, long cost recovery period, poor product color and quality, etc. Acid conversion rate and extraction yield, huge economic and environmental benefits, and the effect of reducing the burden of sewage treatment
Active Publication Date: 2017-08-04
TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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[0003] When direct fermentation is used to produce threonine, the traditional fermentation medium contains corn steep liquor and yeast powder, and its components are complex, which will cause a large amount of foam in the fermentation process, affecting oxygen transfer, and it contains pigments and other impurities, making threonine During the acid extraction process, the concentration ratio is small, the extraction yield and sugar-acid conversion rate are low, the color and quality of the product are poor, and a large amount of mother liquor remains
[0004] The existing threonine mother liquor treatment methods include: (1) directly entering the sewage treatment
Method used
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[0024] Example 1
[0025] A threonine fermentation medium, including: glucose 30g / L, MgSO 4 ·7H 2 O 0.8g / L, FeSO 4 ·7H 2 O5mg / L, biotin 0.2mg / L, V B1 0.3mg / L, MnSO 4 ·H 2 O 3mg / L, KH 2 PO 4 1.5g / L, citric acid 2g / L, glutamic acid and lysine each 0.5g / L, glycine, tyrosine, aspartic acid, arginine, methionine each 0.1g / L, bird 0.1g / L each of glycoside, inosine, and adenosine, 5mL / L of trace elements (the final concentration of trace elements in the medium is CoCl 2 ·6H 2 O 1.4g / L, MnSO 4 ·H 2 O 0.5g / L, CuSO 4 ·7H 2 O 0.5g / L), the rest is deionized water.
[0026] The preparation method of the above threonine fermentation medium is as follows: press glucose 30g / L, MgSO 4 ·7H 2 O 0.8g / L, FeSO 4 ·7H 2 O 5mg / L, biotin 0.2mg / L, V B1 0.3mg / L, MnSO 4 ·H 2 O 3mg / L, KH 2 PO 4 1.5g / L, citric acid 2g / L, glutamic acid and lysine each 0.5g / L, glycine, tyrosine, aspartic acid, arginine, methionine each 0.1g / L, bird Glucoside, inosine, and adenosine are each 0.1g / L, and the amount of trace elem...
Example Embodiment
[0027] Example 2
[0028] A clean production process of threonine, including the following steps:
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Abstract
The invention provides a threonine fermentation medium and a clean production process of threonine; the threonine fermentation medium is free of corn steep liquor and yeast powder; substitution is achieved by adding various amino acids, nucleotide and trace elements; the threonine fermentation medium is used to ferment threonine clear liquor, sugar-acid conversion rate and extracting yield are increased, and threonine can be cleanly produced with very excellent effect; after 26 hours of fermentation, the yield of threonine reaches 128.6 g/L, froth is lessened during fermentation, the usage of Pao Di (a defoaming agent) is reduced, stirring output power is increased, the concentration of acetic acid in fermentation broth is decreased from 12 g/L to 3 g/L, pigments and ash content in the medium are decreased, concentration times of the fermentation broth during concentrative crystallization is increased, the volume of crystal mother liquor is decreased from 8% of the original process to 3%, the yield is increased from 82% to 94%, sugar-acid conversion rate is increased from 56% to 59%, the product purity is greater than 99.2%, and the product color and quality are improved greatly.
Description
technical field [0001] The invention relates to the technical field of amino acid production by industrial fermentation, in particular to a threonine fermentation medium and a threonine clean production process. Background technique [0002] The chemical name of threonine is α-amino-β-hydroxybutyric acid, which is one of the essential amino acids for the human body. The human body cannot synthesize it by itself and must be ingested from food. As a food and feed additive, it is widely used in feed, health food and medicine industry. The production methods of threonine include protein hydrolysis, chemical synthesis and microbial fermentation. Due to various disadvantages of protein hydrolysis and chemical synthesis, industrial production is basically no longer used. The direct fermentation method has gradually become the main way for the industrial production of threonine due to its advantages of low production cost, resource conservation, and less environmental pollution. ...
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IPC IPC(8): C12P13/08C12R1/19
CPCC12P13/08
Inventor 徐庆阳陈宁蔡萌萌杨梦晨户红通刘子强刘镇瑜谢希贤张成林李燕军范晓光
Owner TIANJIN UNIVERSITY OF SCIENCE AND TECHNOLOGY
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