Threonine fermentation medium and clean production process of threonine

Abstract

The invention provides a threonine fermentation medium and a clean production process of threonine; the threonine fermentation medium is free of corn steep liquor and yeast powder; substitution is achieved by adding various amino acids, nucleotide and trace elements; the threonine fermentation medium is used to ferment threonine clear liquor, sugar-acid conversion rate and extracting yield are increased, and threonine can be cleanly produced with very excellent effect; after 26 hours of fermentation, the yield of threonine reaches 128.6 g / L, froth is lessened during fermentation, the usage of Pao Di (a defoaming agent) is reduced, stirring output power is increased, the concentration of acetic acid in fermentation broth is decreased from 12 g / L to 3 g / L, pigments and ash content in the medium are decreased, concentration times of the fermentation broth during concentrative crystallization is increased, the volume of crystal mother liquor is decreased from 8% of the original process to 3%, the yield is increased from 82% to 94%, sugar-acid conversion rate is increased from 56% to 59%, the product purity is greater than 99.2%, and the product color and quality are improved greatly.

Description

technical field [0001] The invention relates to the technical field of amino acid production by industrial fermentation, in particular to a threonine fermentation medium and a threonine clean production process. Background technique [0002] The chemical name of threonine is α-amino-β-hydroxybutyric acid, which is one of the essential amino acids for the human body. The human body cannot synthesize it by itself and must be ingested from food. As a food and feed additive, it is widely used in feed, health food and medicine industry. The production methods of threonine include protein hydrolysis, chemical synthesis and microbial fermentation. Due to various disadvantages of protein hydrolysis and chemical synthesis, industrial production is basically no longer used. The direct fermentation method has gradually become the main way for the industrial production of threonine due to its advantages of low production cost, resource conservation, and less environmental pollution. ...

AI Technical Summary

Problems solved by technology

[0003] When direct fermentation is used to produce threonine, the traditional fermentation medium contains corn steep liquor and yeast powder, and its components are complex, which will cause a large amount of foam in the fermentation process, affecting oxygen transfer, and it contains pigments and other impurities, making threonine During the acid extraction process, the concentration ratio is small, the extraction yield and sugar-acid conversion rate are low, the color and quality of the product are poor, and a large amount of mother liquor remains

[0004] The existing threonine mother liquor treatment methods include: (1) directly entering the sewage treatment system, which will lead to a low extraction yield of threonine; Acid, this method can improve threonine yield, but can consume a large amount of liquid ammonia and sulfuric acid, make production cost increase; (3) adopt more advanced chromatographic separation method, this method can also reach high yield, but chromatographic The one-time investment of separation is large, and the cost recovery period is long. At the same time, this method requires pretreatment of raw materials, which will consume a large amount of deionized water, and the obtained extract has low threonine content, and a large amount of energy will be consumed during the concentration process.