32 results about "Actinoplanes sp." patented technology

The present invention utilizes three families of bacterial enzymes, which play a key role in mycothiol biosynthesis. The three families are bacterial cysteine:glucosaminyl inositol ligases (MshC) with catalytic ligase activity for ligation of glucosaminyl inositol and cysteine, bacterial acetyl-CoA:Cys-GlcN-Ins acetyltransferases (MshD) with catalytic activity for addition of an acetyl group to Cys-GlcN-Ins and bacterial MshA glycosyltransferase with catalytic activity for production of GlcNAc-Ins. The invention provides methods for using the mycothiol biosynthesis ligases, acetyltransferases or glycosyltransferases in drug screening assays to determine compounds that inhibit activity. The invention provides for treatment of actinomycete infections in mammals using antibiotics that inhibit production or activity of the enzymes of mycothiol biosynthesis, in particular MshC, MshD or MshA, and thereby reduce the production of mycothiol and the virulence of the infecting bacteria. Additionally, the invention provides a live mutant with a genome containing a modification in an endogenous enzyme of mycothiol biosynthesis gene. The invention also provides an expression vector comprising polynucleotides of mshA, mshB, mshC and mshD.

The invention discloses a high-yield fidaxomicin genetically engineered bacterium Actinomycetes deccanensis YP‑2 (Actinoplanes deccanensis YP‑2) and a construction method. Among the Actinomycetes Deccanum YP‑1, the genetically engineered Actinomycetes YP‑2 with a high yield of fidaxomicin was screened out, and the yield was 400% to 500% higher than that of the original strain, up to 130mg / L, has a good application prospect. The method of the invention is efficient, accurate and easy to operate, and provides a new research method for the construction of the efficient biosynthesis pathway of the actinomycete drug. The fidaxomicin genetically engineered bacterium Actinomyces deccanum YP‑2 can be used in the preparation of medicines for treating diarrhea caused by Clostridium difficile infection.

The invention discloses a method of utilizing an Actinoplanes sp. fermentation culture material to prepare three ramoplanin single components. The method includes: conducting decoloration on a ramoplanin fermentation solution through macroporous decolorization resin, then carrying out macroporous resin adsorption, desorption as well as concentration so as to obtain a ramoplanin crude extract containing the three single components: A1, A2, and A3; and dissolving the crude extract with a solvent, then performing chromatographic separation by means of a polymer microsphere, thus obtaining the products of three high purity ramoplanin single components A1, A2 and A3. The invention has the advantage that when using the polymer microsphere to perform chromatographic separation on the product, the sample treatment amount is large, and the obtained single components have high purity. As the price of a polymer microsphere medium is 30% of that of the reverse phase silica gel C18, and the separation effect is equivalent, the preparation cost is greatly reduced.

Synthesis and characterization of novel DACT forms suitable for pharmaceutical compositions in drug delivery systems to treat cancer in humans or a warm-blooded mammals. The novel forms include but not limited to cocrystals, salts, solvates of salts, and mixtures thereof.

The invention discloses an alpha-amylase inhibitor producing strain, i.e. Actinoplanes sp.ZJB-08196 preserved in the China Center for Type Culture Collection located in Wuhan university, Wuhan Province, China on Feb. 16th, 2009 with the preservation number of CCTCC No. M209022. The alpha-amylase inhibitor is acarbose. The invention also discloses a screening method of the alpha-amylase inhibitor producing strain. The screening method provided by the invention is simple and effective, can screen a great amount of samples in a short time, has high sensitivity, reduces the long processes for processing and analyzing samples through HPLC (High-Performance Liquid Chromatography), and the like and improves detection efficiency.

A method for improving the biosynthetic yield of ansamitocin P-3, by constructing the expression plasmid pIB139-asm13-17 of the asm13-17 gene, constructing the expression plasmid pIB139-asmUdpg of the asmUdpg gene, and constructing the co-expression of the asm13-17 gene and asmUdpg The integrated expression plasmid pIB139‑asm13‑17:asmUdpg corresponding to the gene, and the above expression plasmids are respectively transferred into Actinosynnema pretiosum precious orange (Actinosynnema pretiosum), to achieve the improvement of biosynthetic expression, the present invention can significantly improve Fermentation levels of ansamitocin P‑3.

The invention provides two Actinoplanaceae strains and antibacterial application thereof. The Actinoplanaceae strains, Actinoplanes sp. PDF-1 and PDF-2, have preserving numbers of CGMCC No. 4692 and CGMCC No. 4693. The strains have effect in resisting bacteria, especially resisting drug-resistant bacteria. The strains provided by the invention are renewable microbe resources, can obtain products having activity in resisting drug-resistant bacteria, are suitable for large-scale industrial fermentation production, and have environmental pollution smaller than chemical synthesis.