1 results about "Actinoplanes sp." patented technology

This invention discloses a high-yield S-type amino acid ester imine reductase mutant, its preparation method, and its applications. The wild-type imine reductase is derived from actinomycetes. Actinomadura welshii By using directed evolution, the active site was modified to obtain imine reductase mutants with high activity, stereoselectivity, and (S)-2-((4-methoxyphenyl)amino)-2-phenylacetic acid methyl ester yield. The mutants are D98G / M122L / W216V, D98G / M122L / W216V / L15Y, D98G / M122L / W216V / L15Y / A120V, and D98G / M122L / W216V / L15Y / A120V / V124L / T238A. This invention enables the efficient synthesis of (S)-2-((4-methoxyphenyl)imino)-2-phenylacetic acid methyl ester from inexpensive (trans)-2-((4-methoxyphenyl)amino)-2-phenylacetic acid methyl ester, which is beneficial for the industrial synthesis and application of (S)-2-((4-methoxyphenyl)amino)-2-phenylacetic acid methyl ester.