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Two Actinoplanaceae strains and antibacterial application thereof

A swimming actinomycetes, anti-bacterial technology, applied in the field of microorganisms, can solve the problems of low economic benefits in the pharmaceutical industry, and achieve the effect of chemical synthesis

Active Publication Date: 2013-01-02
MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, because about 97% of the drugs produced by the pharmaceutical industry are imitated foreign varieties, although my country can be called a big country in pharmaceutical production, it is still a weak country in medical technology, which makes the overall economic benefits of the pharmaceutical industry low

Method used

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  • Two Actinoplanaceae strains and antibacterial application thereof
  • Two Actinoplanaceae strains and antibacterial application thereof

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0019] Embodiment 1: the preparation of bacterial strain fermentation product

[0020] The fermentation product preparation process of bacterial strain is as follows: Actinoplanes sp.PDF-1 and PDF-2 pure culture are respectively inoculated to slant medium (yeast extract 0.4%, malt extract 1.0%, glucose 0.4%, agar 1.5%, pH7 .3) at 28°C for 10 days. Pick a 0.5 cm square piece of culture species from the slope and put it into 50ml fermentation medium (glucose 0.5%, malt extract 1%, cottonseed cake powder 1%, soluble starch 2%, yeast extract 0.5%, K 2 HPO 4 0.05%, (NH 4 ) 2 SO 4 0.5%, NaCl0.1%, CaCO 3 0.3%, pH7.2. ) in a 250ml Erlenmeyer flask at 28°C and 190 rpm on a rotary shaker for 4 days. Take 50ml of fermentation broth, extract it with 50ml of acetone, evaporate to dryness, and dissolve it with 5ml of DMSO.

Embodiment 2

[0021] Embodiment 2: verification of antibacterial activity

[0022] The agar dilution method was used to determine the antibacterial activity of the acetone crude extract from the fermentation broth. The tested strains included sensitive strains of Staphylococcus aureus, methicillin-resistant Staphylococcus aureus, sensitive strains of Staphylococcus epidermidis, and methicillin-resistant Staphylococcus aureus cocci, susceptible strains of Enterococcus faecalis, vancomycin-resistant E. Wait for 20 strains of bacteria.

[0023] 1. Prepare culture medium

[0024] The culture medium is Mueller-Hinton (MH) agar medium, which is prepared according to the product instructions, and has a pH of 7.2-7.4.

[0025] 2. Cultivation of tested strains

[0026] 20 tested strains were cultured overnight at 37°C.

[0027] 3. Preparation of drug-containing tablet

[0028] Add 1ml of fermented broth crude extract samples to 14ml of MH agar medium at a temperature of 45-50°C and mix well, th...

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Abstract

The invention provides two Actinoplanaceae strains and antibacterial application thereof. The Actinoplanaceae strains, Actinoplanes sp. PDF-1 and PDF-2, have preserving numbers of CGMCC No. 4692 and CGMCC No. 4693. The strains have effect in resisting bacteria, especially resisting drug-resistant bacteria. The strains provided by the invention are renewable microbe resources, can obtain products having activity in resisting drug-resistant bacteria, are suitable for large-scale industrial fermentation production, and have environmental pollution smaller than chemical synthesis.

Description

technical field [0001] The invention relates to the technical field of microorganisms, in particular to two strains of actinomycetes and their application in anti-bacteria. Background technique [0002] A recent survey showed that 65% of nosocomial bacterial infections in 47 American hospitals were caused by Gram-positive bacteria, the most important of which were Staphylococcus aureus and Enterococcus. With the widespread use of antibacterial drugs, the detection rate and drug resistance rate of Staphylococcus, especially methicillin-resistant Staphylococcus aureus (MRSA), are increasing year by year. Vancomycin, a glycopeptide antibiotic discovered in the 1950s, was used as the drug of choice for the treatment of multidrug-resistant Gram-positive bacterial infections due to its unique effects, and was once hailed as "human's response to intractable multidrug resistance." The last line of defense against Gram-positive infections." But this last line of defense is also in ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A61K35/74A61P31/04C12R1/045
Inventor 余利岩张月琴董国霞李子强孙薇张玉琴李秋萍魏玉珍
Owner MEDICINE & BIOENG INST OF CHINESE ACAD OF MEDICAL SCI
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