Microbial fodder additive and its preparing method and use

A technology of microbial feed and additives, applied in animal feed, animal feed, application, etc., can solve the problems of unstable efficacy, short survival time of viable cells, and single function.

Active Publication Date: 2005-10-19
CHINA AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

[0007] The purpose of the present invention is to use three types of 4 strains of beneficial functional microorganisms including lactobacillus, saccharomyces and bacillus to rationally compound compatibility, adopt liquid submerged fermentation technology and a series of post-processing technology to overcome the existing microbial feed addit

Method used

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  • Microbial fodder additive and its preparing method and use

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0087] Example 1. Fermentation broth for culturing Bacillus subtilis (A)

[0088] 1) Plate culture and rejuvenation: The preserved strains of Bacillus subtilis are streaked on the Bacillus plate medium by streaking inoculation, and cultured at 30°C for 18 hours to rejuvenate Bacillus subtilis and form a single colony; The Bacillus subtilis culture medium is a mixture prepared in the following ratio: peptone 0.5% (W / V, the same below), glucose 0.5%, beef extract 0.5%, potassium dihydrogen phosphate 0.3%, agar 3%, pH= 7.5~7.6, sterilize at 121℃ for 30 minutes, spare;

[0089]2) Making seeds of shake flasks (triangular flasks): select a typical single colony from the Bacillus subtilis colonies cultured in step 1), inoculate it into Bacillus liquid culture medium, and cultivate at 32°C for 30 hours to obtain Bacillus subtilis shake flasks Seed liquid; the bacillus liquid medium is a mixed liquid prepared in the following proportions: peptone 0.5%, glucose 2%, beef extract 0.5%, potass...

Embodiment 2

[0092] Example 2. Fermentation broth for culturing Bacillus subtilis (A)

[0093] 1) Plate culture and rejuvenation: The preserved strains of Bacillus subtilis are streaked on the Bacillus plate medium by streaking inoculation, and cultured at 30°C for 18 hours to rejuvenate Bacillus subtilis and form a single colony; The Bacillus subtilis culture medium is a mixture prepared in the following proportions: peptone 0.5% (% refers to weight and volume percentage, the same below), glucose 0.5%, beef extract 0.5%, potassium dihydrogen phosphate 0.3%, agar 3%, pH=7.5~7.6, sterilize at 121°C for 30 minutes, ready for use;

[0094] 2) Making the seeds of the shake flask (triangle flask): select a typical single colony from the Bacillus subtilis colonies cultivated in step 1), inoculate it into the Bacillus liquid medium, and cultivate it at 32°C for 30 hours to obtain the Bacillus subtilis shake flask Seed liquid; the bacillus liquid medium is a mixed liquid prepared in the following prop...

Embodiment 3

[0098] Example 3. Fermentation broth (B) for culturing Bacillus cereus

[0099] The type of microorganism is Bacillus cereus. The culture medium, conditions, and preparation methods are the same as those in Example 1. The fermentation broth of Bacillus cereus is obtained. Finally, according to the medium and culture conditions used for plate culture and rejuvenation of Bacillus cereus, The content of viable bacteria in the fermentation broth of Bacillus cereus is tested by conventional plate counting. According to the count results, the viable bacteria content is diluted to 10 with sterilized tap water 8 CFU / ml, 10 9 CFU / ml and 10 10 CFU / ml, to obtain the standby liquid (B) of the fermentation broth of Bacillus cereus, to be post-processed.

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Abstract

The present invention relates to microbial fodder additive, and a kind of microbial fodder additive comprising beneficial microbes and their metabolites and its preparation process and use. The microbial fodder additive is the mixture of fermented liquid or dry powder obtained with Bacillus subtilis, Bacillus cereus, lactobacillus and Saccharomyices cerevisiae and through high density deep layer liquid fermentation. It may be prepared into different specific liquid or powder preparations. The microbial fodder additive has high live bacillus content, long product preserving period and less foreign bacteria contamination, and has the functions of raise fodder utilization, promote animal's growth, increase animal product yield and quality, etc.

Description

technical field [0001] The invention relates to a microbial feed additive, in particular to a microbial feed additive composed of various beneficial microorganisms and their metabolites (such as protease, lipase, amylase, cellulase, etc.), its preparation method and application. Background technique [0002] The use of some growth-promoting health care agents in feed industry and breeding industry has promoted the rapid development of animal husbandry, but at the same time it has brought many potential threats to human health and the development of feed industry. At present, the abuse of antibiotics, chemical synthetic drugs, hormones, β-stimulants, heavy metals, sedatives and other growth-promoting health care agents in feed and livestock and poultry breeding has caused a series of problems and consequences: (1) leading to serious drug residues in livestock and poultry products, directly endangering human health, and even causing "three diseases", and making my country's liv...

Claims

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Application Information

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IPC IPC(8): A23K10/18C12N1/20
Inventor 张日俊
Owner CHINA AGRI UNIV
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