Culture method of Aspergillus niger for producing citric acid

A technology of Aspergillus niger and citric acid, applied in microorganism-based methods, biochemical equipment and methods, microorganisms, etc., can solve the problems of low fermentation efficiency of Aspergillus niger and the growth state of Aspergillus niger, and achieve a stable and high fermentation efficiency. Effect

Active Publication Date: 2013-01-02
COFCO BIOTECHNOLOGY CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] The purpose of the present invention is to overcome the disadvantages that the fermentation efficiency of the Aspergillus niger cultivated by the existing method is low, and cannot truly reflect the growth state of the Aspergillus niger and is greatly influenced by human factors, and provides a method to make the Aspergillus niger in the follow-up The cultivation method of Aspergillus niger which has higher fermentation efficiency and can accurately judge the growth state of Aspergillus niger in the fermentation process

Method used

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Examples

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preparation example Construction

[0013] According to the present invention, the preparation method of the Aspergillus niger culture solution is not particularly limited, as long as the obtained culture solution can be suitable for the cultivation of Aspergillus niger, for example, the preparation method of the Aspergillus niger culture solution may include: Add amylase, heat up to 90-100°C at a rate of 20-150°C / hour and spray once at this temperature, flash after 5-30 minutes, and wait until the temperature drops to 80-95°C at this Enzymatically hydrolyzing the starch at high temperature for 90-140 minutes to obtain a liquefied liquid and diluting the total sugar to 5-20% by weight, then adding nitrogen source and sterilizing. The term total sugar refers to the total content of sugar contained in the enzymatic hydrolysis liquefaction solution.

[0014] According to the present invention, in the starch slurry, the contents of starch and water and the pH of the slurry can be changed in a wide range, preferably,...

Embodiment 1

[0029] (1) Dry corn flour and water are slurried, and the ratio of dry corn flour and water makes in the obtained slurry, the content of starch is 15% by weight, and the content of water is 85% by weight, and the pH value of slurry is controlled with sodium hydroxide 6.2, with respect to the starch of 1000 parts by weight, add 0.6 parts by weight of amylase (Novozymes, α-amylase), and raise the temperature to 96°C at a speed of 40°C / hour, and carry out once at this temperature Jet liquefaction, flash evaporation after 10 minutes, and when the temperature drops to 90°C, enzymatically hydrolyze the starch for 100 minutes at this temperature to obtain a liquefaction solution.

[0030] (2) Get 7.5% by weight of the liquefied liquid obtained in step (1), dilute it with water until the total sugar concentration is 10% by weight, and then put it into the seed tank, add urea, and the amount of urea added is 0.35% of the total weight of the seed tank culture solution , heated to 120°C ...

Embodiment 2

[0036] (1) Dry corn flour and water are slurried, the ratio of dry corn flour and water makes in the obtained slurry, the content of starch is 25% by weight, the content of water is 75% by weight, and the pH value of the control slurry is 5.5, With respect to 1000 parts by weight of starch, add 0.5 parts by weight of amylase (Novozymes, α-amylase), and heat up to 100°C at a rate of 80°C / hour, and perform a jet liquefaction at this temperature, Flash evaporation was carried out after 15 minutes, and when the temperature dropped to 80° C., the starch was enzymatically hydrolyzed at this temperature for 120 minutes to obtain a liquefied liquid.

[0037] (2) Get 7.5% by weight of the liquefied liquid obtained in step (1), add water to dilute to 10% of total sugar and drop into the seed tank, add ammonium nitrate, the addition of ammonium nitrate is 0.1% of the total weight of the seed tank culture solution, heat Sterilize at 120°C, maintain for 20 minutes and then quickly cool dow...

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Abstract

The invention provides a culture method of Aspergillus niger for producing citric acid. The method comprises the following steps: inoculating Aspergillus niger in an Aspergillus niger culture solution for culture, wherein the respiratory quotient of the Aspergillus niger thallus is 0.7-1.5 under the conditions of culture; and maintaining for 2-8 hours at the respiratory quotient. According to themethod provided by the invention, whether the growth state of the Aspergillus niger is applicable to subsequent fermentation is judged according to the numerical value of the respiratory quotient, and culture is maintained for 2-8 hours at the state that the respiratory quotient is 0.7-1.5, so that the obtained Aspergillus niger has high fermentation efficiency in the subsequent fermentation process; and the method provided by the invention is slightly influenced by personal factors, thereby maintaining the stability of Aspergillus niger culture among batches.

Description

technical field [0001] The present invention relates to a method for cultivating Aspergillus niger for producing citric acid. Background technique [0002] Citric acid is an organic acid widely used in beverage, food and pharmaceutical industries. my country is a major producer of citric acid, with more than 20 production plants, and the output has exceeded 800,000 tons. Before carrying out fermentation to prepare citric acid, it is usually necessary to cultivate the Aspergillus niger used for producing citric acid, so as to expand the amount of Aspergillus niger and make its growth state suitable for fermentation. [0003] At present, when Aspergillus niger is cultured before fermentation, it is usually judged by observation whether the growth of Aspergillus niger meets the fermentation application for producing citric acid, but the fermentation efficiency of Aspergillus niger cultivated by the existing method is low, and by Observation cannot truly reflect the growth stat...

Claims

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Application Information

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Patent Type & Authority Patents(China)
IPC IPC(8): C12N1/14C12P7/48C12R1/685
Inventor 周永生周勇王永红杨儒文章辉平
Owner COFCO BIOTECHNOLOGY CO LTD
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