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Method for preparing 1,5-pentanediamine by fermentation

A technology of pentamethylenediamine and fermentation tank, which is applied in the field of fermentation and can solve problems such as complex processes

Active Publication Date: 2019-11-19
CATHAY R&D CENT CO LTD +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

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Problems solved by technology

Although this method does not require the purification of lysine, it requires the production and purification of lysine decarboxylase, which is a very complicated process

Method used

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  • Method for preparing 1,5-pentanediamine by fermentation
  • Method for preparing 1,5-pentanediamine by fermentation
  • Method for preparing 1,5-pentanediamine by fermentation

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0068] Seed medium: KH 2 PO 4 0.2% (w / v, the following each embodiment, each medium component of comparative example is the same; 0.2% is 2g / L), MgSO 4 ·7H 2 O 0.45%, MnSO 4 ·H 2 O 20ppm, ammonium sulfate 0.5%, glucose 10%, corn steep liquor 0.15% (total nitrogen content 2.5wt%, the same below), threonine 0.035%; fermentation medium: KH 2 PO 4 0.04%, MgSO 4 ·7H 2 O0.32%, MnSO 4 ·H 2 O 0.028%, ammonium sulfate 1.0%, glucose 3.0%, corn steep liquor 0.26%, threonine 0.017%; fermentation feed medium: glucose 60%, ammonium sulfate 20%, threonine 0.1%.

[0069] The seed tank cultivation method is: 10L fermenter (working volume 4L), inoculation ratio 0.5% (v / v, the following examples and comparative examples are the same), aeration ratio 0.4vvm, temperature 35°C, rotating speed 300rpm, tank pressure 0.05MPa , 25% (v / v) ammonia water control pH is 6.0, in bacterial concentration OD 562 After reaching 0.75 (diluted 25 times), it was connected to a fermenter.

[0070] The fe...

Embodiment 2

[0074] Seed medium: KH 2 PO 4 0.5%, MgSO 4 ·7H 2 O 0.15%, MnSO 4 ·H 2 O 10ppm, ammonium sulfate 2.5%, glucose 5%, corn steep liquor 0.30%, threonine 0.015%; fermentation medium: KH 2 PO 4 0.02%, MgSO 4 ·7H 2 O 0.50%, MnSO 4 ·H 2O 0.010%, ammonium sulfate 0.2%, glucose 1.0%, corn steep liquor 0.50%, threonine 0.010%; fermentation feed medium: glucose 50%, ammonium sulfate 60%, threonine 1.0%.

[0075] The seed tank culture method is: 10L fermenter (working volume 5L), inoculum ratio 1.2%, aeration ratio 0.8vvm, temperature 39°C, rotating speed 800rpm, tank pressure 0.15MPa, 28% (v / v) ammonia water control pH is 7.0, OD 562 After reaching 1.00 (diluted 25 times), it was connected to a fermenter.

[0076] The fermenter culture method is: 30L fermenter (working volume 15L), inoculum ratio 5%, aeration ratio 0.8vvm, temperature 39°C, rotating speed 800rpm, tank pressure 0.05MPa, 28% (v / v) ammonia water control pH is 7.0, Feed medium was added after 2 h of fermentatio...

Embodiment 3

[0081] Seed medium: KH 2 PO 4 0.35%, MgSO 4 ·7H 2 O 0.28%, MnSO 4 ·H 2 O 15ppm, ammonium sulfate 1.7%, glucose 15%, corn steep liquor 0.05%, threonine 0.045%; fermentation medium: KH 2 PO 4 0.06%, MgSO 4 ·7H 2 O 0.10%, MnSO 4 ·H 2 O 0.050%, ammonium sulfate 0.5%, glucose 6.0%, corn steep liquor 0.10%, threonine 0.030%; fermentation feed medium: glucose 80%, ammonium sulfate 40%, threonine 0.5%.

[0082] The seed tank cultivation method is: 10L fermenter (working volume 5L), inoculum ratio 0.8%, aeration ratio 0.6vvm, temperature 37°C, rotating speed 500rpm, tank pressure 0.10MPa, 26% (v / v) ammonia water control pH is 6.5, OD 562 After reaching 0.75, connect to the fermenter.

[0083] The fermenter culture method is: 5L fermenter (working volume 3L), inoculum ratio 12%, aeration ratio 0.4vvm, temperature 35°C, rotating speed 500rpm, tank pressure 0.2MPa, 26% (v / v) ammonia water control pH is 6.0, Feed medium was added after 6 h of fermentation. The control RQ in...

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Abstract

The invention relates to a fermentation method and particularly relates to a method for preparing 1,5-pentanediamine by fermentation. The method for preparing 1,5-pentanediamine by fermentation comprises the steps of converting a substrate into 1,5-pentanediamine through bacterial fermentation and controlling RQ (respiratory quotient) to be 1.2-2.2 by controlling a speed for adding ammonium sulfate in stages in the fermenting process. The method can solve the technical problem of low pentanediamine conversion rate in a current preparation method.

Description

technical field [0001] The invention relates to a fermentation method, in particular to a method for producing 1,5-pentanediamine by fermentation. Background technique [0002] Pentylenediamine (Diaminopentane; DN), also known as cadaverine, is widely used in agriculture, medicine and industry. In agriculture, exogenous application of DN can improve fruit setting and promote fruit ripening; in medicine, DN can be used as an effective drug for treating dysentery; in industry, pentamethylenediamine can replace diamines from petrochemical products Polymerization produces polyamide. At present, the annual output of polyamide is close to 6 million tons, among which nylon 6.6 (Polyamide 6.6; PA 6.6) is one of the earliest developed, largest and most widely used polyamide products, and is widely used in aerospace, automotive parts, Mechanical parts, electronic appliances and packaging materials and other industries. PA 6.6 is produced by the 1:1 polymerization of adipic acid and...

Claims

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Application Information

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IPC IPC(8): C12P13/00C12N1/20C12R1/19
CPCC12P13/001C12N1/20
Inventor 路飞周豪宏刘修才
Owner CATHAY R&D CENT CO LTD
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