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Method and device for optimizing and amplifying fermentation process

A process optimization and data amplification technology, applied in biochemical cleaning devices, biochemical equipment and methods, fermentation, etc., can solve the problems of lack of optimization and amplification methods, no characteristic parameters of cell metabolite flow distribution changes, etc.

Inactive Publication Date: 2015-07-15
EAST CHINA UNIV OF SCI & TECH
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  • Abstract
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  • Application Information

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Problems solved by technology

[0015] However, the shortcomings of the above-mentioned existing amplification criteria are that they are all based on the static operation method based on the optimal process control point based on the detection of extracellular parameters, and do not focus on the relevant phenomena based on the change of the distribution of the flow of the metabolic substance in the cell. Characteristic Parameters
[0017] In summary, the art lacks a method for optimizing and scaling up the fermentation process of cephalosporin C, and an industrial-scale bioreactor for the fermentation of cephalosporin C

Method used

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  • Method and device for optimizing and amplifying fermentation process
  • Method and device for optimizing and amplifying fermentation process
  • Method and device for optimizing and amplifying fermentation process

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Experimental program
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Embodiment 1

[0119] 1 Materials and methods

[0120] 1.1 Experimental strains

[0121] Cephalosporium Acremonium AC0508 was provided by Shanxi Weiqida Pharmaceutical Co., Ltd.

[0122] 1.2 Medium

[0123] 1.2.1 Shake flask culture

[0124] Incline medium (100ml): wort juice 1.2g, peptone 1.2g, agar 2.2g; pH before digestion is 7.0;

[0125] Seed medium (100ml): corn steep liquor 3g, ammonium acetate 0.4g, sucrose 2g, DL-methionine 0.02g, CaCO 3 0.05g; pH before elimination is 6.5; (superscript, subscript)

[0126] Fermentation medium (100ml): corn steep liquor 7g, starch 4g, amylase 0.03g, soybean oil 1g, DL-methionine 0.2g, KH 2 PO 4 0.4g, (NH4) 2 SO 4 0.8g, FeSO 4 ·7H 2 00.005g, CaCO 3 lg; the pH before digestion was 6.2.

[0127] 1.2.2 Fermentation tank culture

[0128] Primary seed medium (100L): glucose 1kg, sucrose 2.5kg, corn steep liquor 1kg, bean cake powder 3kg, calcium carbonate 0.5kg, defoamer 0.08kg, pH 6.0 before elimination;

[0129] Secondary seed medium (100L...

Embodiment 2

[0176] Such as Figure 9 Shown, self-control bioreactor device of the present invention comprises the bioreactor tank body (100) that has stirrer (104), and this device is by the fermenter tank body of belt motor and stirrer (104), has multi-parameter detection and It consists of a sensor system for controlled instruments and sensors, a mounting bracket and a process piping system, and an electrical control cabinet with an industrial computer and executive components, wherein the sensor system includes a temperature sensor (1), a pH Sensor(2), Dissolved Oxygen Sensor(3), Full Tank Scale Sensor(4), Exhaust Gas CO 2 Port (5), exhaust gas O 2 Interface (6), velocity sensor (7), pressure sensor (8), defoaming sensor (9), ventilation flow sensor (26), live cell mass sensor (10); Described parameter refers to temperature, stirring speed, Ventilation flow, tank pressure, defoaming, pH, dissolved oxygen concentration, real volume and weight of fermentation broth, feeding amount incl...

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Abstract

The invention provides a method for process optimization and data amplification for bioreactors. The method comprises the following steps: (a) measuring the physiological metabolism parameter, and physiologic metabolism parameter correlation related characteristics or combination thereof of each bioreactor, wherein the physiologic metabolism parameter is selected from oxygen dissolution rate, oxygen intake rate, respiratory quotient, volume oxygen transfer coefficient or combination thereof; (b) comparing the physiologic metabolism parameter and physiologic metabolism parameter related characteristics measured in step (a) with the preset physiologic metabolism parameter, and physiologic metabolism parameter related characteristics or combination thereof; selecting the bioreactor of which the physiologic metabolism parameter and physiologic metabolism parameter related characteristics most approaching to the preset values; and determining the optimized amplification bioreactor, wherein the physiologic metabolism parameter is specified in step (a).

Description

[0001] The applicant of this divisional application filed on September 11, 2008 is a divisional application of an invention patent application with the application number 200810042759.0 and the title of the invention "A Method and Device for Optimizing and Enlarging a Fermentation Process". technical field [0002] The invention relates to a method and device for optimizing and amplifying a fermentation process, in particular to a method and a device for optimizing and amplifying a cephalosporin C fermentation process. Background technique [0003] In order to make the rules and data obtained from small-scale fermentation experiments reproduced in large-scale production, it is necessary to master and use amplification technology. In the prior art, a similar principle is generally used for comparison and amplification. Specifically, the basic method of comparison and amplification in the prior art is: firstly, it is necessary to find out various parameters characterizing the ...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P35/06C12M1/38C12M1/36C12M1/34C12M1/02
CPCC12M41/34C12M41/32
Inventor 储炬庄英萍张嗣良李景森王永红黄明志杭海峰黎亮
Owner EAST CHINA UNIV OF SCI & TECH
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