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Control method for producing gluconate by microbial method

A technology of gluconate and control method, which is applied in the control field of microbial production of gluconate, which can solve the problems of lack and failure to observe the growth period of gluconic acid microorganisms, and achieve the effect of precise control

Active Publication Date: 2011-12-28
山东福洋生物科技股份有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0004] Therefore, the fermentation of gluconic acid and its salts (such as sodium gluconate, calcium gluconate, etc.) , residual sugar concentration and other methods, however, these methods failed to observe the microbial growth period of gluconic acid and its salt fermentation, and failed to apply online RQ to realize the online control feeding strategy for the fermentation process
[0005] In summary, there is currently a lack of online closed-loop control of fed-batch feeding in the fermentation process of gluconic acid and its salts in this field, so it is urgent to develop an online , Accurate determination of microbial physiological metabolic respiratory quotient and method for controlling automatic feeding of substrate glucose

Method used

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  • Control method for producing gluconate by microbial method

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Experimental program
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Embodiment 1

[0013] The first step, seed culture: Add 2.5Kg of glucose, 75g of corn steep liquor, 6.4g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L culture tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.

[0014] The second step, fermentation: add 4.2KG of glucose, 2.8g of magnesium sulfate, 0.28g of potassium dihydrogen phosphate, 1.4g of urea, and 1ml of defoamer to a 50L fermenter. At 38°C, inoculate the cultured seed solution with a 10% inoculation amount. Control dissolved oxygen (DO) ≥ 30, temperature 38 ℃, use 20% calcium carbonate to adjust pH to 5.5, start fermentation.

[0015] The third step, feeding:

[0016] (1). Ingredients: 14Kg of glucose solution, 36g of corn steep liquor, 5.6g of magnesium sulfate, 2.8g of urea, 1ml of defoamer were added to a 30L feeding tank, and the volume was adjusted to 20L, sterilized at 120°C for 15 minutes, and th...

Embodiment 2

[0024] The first step, seed culture: Add 2.5Kg of glucose, 75g of corn steep liquor, 6.4g of potassium dihydrogen phosphate, 2g of urea, and 2ml of antifoaming agent into a 15L culture tank. To 38°C, add 250ml eggplant bottle bacterial suspension, control dissolved oxygen (DO) ≥ 30, cultivate for 15h to obtain seed liquid.

[0025] The second step, fermentation: add 4.2KG of glucose, 2.8g of magnesium sulfate, 0.28g of potassium dihydrogen phosphate, 1.4g of urea, and 1ml of defoamer to a 50L fermenter. At 38°C, inoculate the cultured seed solution with a 10% inoculation amount. Control dissolved oxygen (DO) ≥ 30, temperature 38 ℃, use 32% sodium hydroxide to adjust pH to 5.5, start fermentation.

[0026] The third step, feeding:

[0027] (1). Ingredients: 14Kg of glucose solution, 36g of corn steep liquor, 5.6g of magnesium sulfate, 2.8g of urea, 1ml of defoamer were added to a 30L feeding tank, and the volume was adjusted to 20L, sterilized at 120°C for 15 minutes, and the...

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Abstract

The invention discloses a control method for gluconate production by a microbiological method. A high-yield gluconic acid strain is adopted as an original strain, which is moved into a fermentation tank for fermentation production of gluconate after seed culture. During fermentation, according to an on-line respiratory quotient (RQ), the adding speed and adding amount of glucose can be controlledin real time. The control method of the invention can make the glucose consumption rate reach 12g / L / h, and the conversion rate of production in the fermentation tank reach over 110%, thus realizing automatic flow feeding and substantially reducing the production cost.

Description

technical field [0001] The invention relates to the field of bioengineering, in particular to a method for controlling the production of gluconate by a microbial method. Background technique [0002] Gluconic acid is widely used in food, medicine, chemical industry, water treatment, construction and other industries as bulking agent, coagulant, chelating agent and sour agent; gluconate (such as with sodium, calcium, zinc, ferrous and other metal oxides Synthetic gluconate) can be used as food additives and nutritional supplements. [0003] The production of gluconic acid mainly includes microbial fermentation, electrolysis and catalytic oxidation. Due to the mild conditions and obvious energy saving of microbial method, it is widely used. Among them, microbial fermentation has fungal fermentation and bacterial fermentation process. Since Boutroux first used the oxidation of microorganisms to oxidize glucose into gluconic acid, significant progress has been made in the ferm...

Claims

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Application Information

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IPC IPC(8): C12P39/00C12P7/42C12R1/685C12R1/845C12R1/80
Inventor 赵伟张雷达王健
Owner 山东福洋生物科技股份有限公司
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