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Method and device for optimizing and amplifying fermentation process

A technology for process optimization and data amplification, applied in the direction of microorganism-based methods, biochemical cleaning devices, biochemical equipment and methods, etc., can solve the phenomenon of no characteristic parameters of changes in the distribution of cell metabolic material flow, lack of optimization and amplification methods, etc. question

Inactive Publication Date: 2010-07-14
EAST CHINA UNIV OF SCI & TECH
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Problems solved by technology

[0014] However, the shortcomings of the above-mentioned existing amplification criteria are that they are all based on the static operation method based on the optimal process control point based on the detection of extracellular parameters, and do not focus on the relevant phenomena based on the change of the distribution of the flow of the metabolic substance in the cell. Characteristic Parameters
[0016] In summary, the art lacks a method for optimizing and scaling up the fermentation process of cephalosporin C, and an industrial-scale bioreactor for the fermentation of cephalosporin C

Method used

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  • Method and device for optimizing and amplifying fermentation process
  • Method and device for optimizing and amplifying fermentation process
  • Method and device for optimizing and amplifying fermentation process

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Embodiment 1

[0119] 1 Materials and methods

[0120] 1.1 Experimental strains

[0121] Cephalosporium Acremonium AC0508 was provided by Shanxi Weiqida Pharmaceutical Co., Ltd.

[0122] 1.2 Medium

[0123] 1.2.1 Shake flask culture

[0124] Incline medium (100ml): wort juice 1.2g, peptone 1.2g, agar 2.2g; pH before digestion is 7.0;

[0125] Seed medium (100ml): corn steep liquor 3g, ammonium acetate 0.4g, sucrose 2g, DL-methionine 0.02g, CaCO 3 0.05g; pH before elimination is 6.5; (superscript, subscript)

[0126] Fermentation medium (100ml): corn steep liquor 7g, starch 4g, amylase 0.03g, soybean oil 1g, DL-methionine 0.2g, KH 2 PO 4 0.4g, (NH4) 2 SO 4 0.8g, FeSO 4 ·7H 2 O0.005g, CaCO 3 1g; pH before digestion is 6.2.

[0127] 1.2.2 Fermentation tank culture

[0128] Primary seed medium (100L): glucose 1kg, sucrose 2.5kg, corn steep liquor 1kg, bean cake powder 3kg, calcium carbonate 0.5kg, defoamer 0.08kg, pH 6.0 before elimination;

[0129] Secondary seed medium (100L): gl...

Embodiment 2

[0177] Such as Figure 9 Shown, self-control bioreactor device of the present invention comprises the bioreactor tank body (100) that has stirrer (104), and this device is by the fermenter tank body of belt motor and stirrer (104), has multi-parameter detection and It consists of a sensor system for controlled instruments and sensors, a mounting bracket and a process piping system, and an electrical control cabinet with an industrial computer and executive components, wherein the sensor system includes a temperature sensor (1), a pH Sensor(2), Dissolved Oxygen Sensor(3), Full Tank Scale Sensor(4), Exhaust Gas CO 2 Port (5), exhaust gas O 2 Interface (6), velocity sensor (7), pressure sensor (8), defoaming sensor (9), ventilation flow sensor (26), live cell mass sensor (10); Described parameter refers to temperature, stirring speed, Ventilation flow, tank pressure, defoaming, pH, dissolved oxygen concentration, real volume and weight of fermentation broth, feeding amount incl...

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Abstract

The invention provides a method for process optimization and data amplification for bioreactors. The method comprises the following steps: (a) measuring the physiological metabolism parameter, and physiologic metabolism parameter correlation related characteristics or combination thereof of each bioreactor, wherein the physiologic metabolism parameter is selected from oxygen dissolution rate, oxygen intake rate, respiratory quotient, volume oxygen transfer coefficient or combination thereof; (b) comparing the physiologic metabolism parameter and physiologic metabolism parameter related characteristics measured in step (a) with the preset physiologic metabolism parameter, and physiologic metabolism parameter related characteristics or combination thereof; selecting the bioreactor of which the physiologic metabolism parameter and physiologic metabolism parameter related characteristics most approaching to the preset values; and determining the optimized amplification bioreactor, wherein the physiologic metabolism parameter is specified in step (a).

Description

technical field [0001] The invention relates to a method and device for optimizing and amplifying a fermentation process, in particular to a method and a device for optimizing and amplifying a cephalosporin C fermentation process. Background technique [0002] In order to make the rules and data obtained from small-scale fermentation experiments reproduced in large-scale production, it is necessary to master and use amplification technology. In the prior art, a similar principle is generally used for comparison and amplification. Specifically, the basic method of comparison and amplification in the prior art is: firstly, it is necessary to find out various parameters characterizing the system, compose them into several dimensionless numbers with certain physical meanings, and establish the functional formula between them, and then The constants and exponents contained in this functional formula are obtained in the test equipment by experimental methods, then this relational...

Claims

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Application Information

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IPC IPC(8): C12P35/06C12M1/38C12M1/36C12M1/34C12M1/02C12R1/75
CPCC12M41/34C12M41/32
Inventor 储炬庄英萍张嗣良李景森王永红黄明志杭海峰黎亮
Owner EAST CHINA UNIV OF SCI & TECH
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