Normal temperature fermentation Hirsutella hepialid Chen et Shen, mutation breeding method and fermentation process thereof

A bat moth spore, room temperature fermentation technology, applied in microorganism-based methods, fermentation, fungi and other directions, can solve the problems of inability to obtain economic and social benefits, long production cycle, high cost, easy to control fermentation parameters, The effect of reduced production cost and high success rate

Active Publication Date: 2009-02-25
JIANGXI BAISHEN PHARMA
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

As can be seen from the above, the fermentation production of Trichosporum of bat moths that has been reported at present is all original and non-mutated strains, so its fermentation production process is carried out at a temperature lower than 20 ° C, and The production cycle is long, the energy consumption is high, and the cost of industrialized production is very high, which inhibits the development of the application industry of Trichosporum of the bat moth, making it unable to obtain the maximum economic and social benefits

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0038] Materials used:

[0039] Potato dextrose agar (PDA) slant medium: distilled water, 20% potato infusion, 2% glucose and 2% agar powder.

[0040] MYG liquid medium: 100ml distilled water contains 10g maltose, 4g glucose, 4g yeast powder, natural pH;

[0041] Composite enzyme: 2.5% Lywallzyme and 0.5% Collapsing Enzyme are mixed in a ratio of 1:1; 2.5% Lywallzyme (Lywallzyme, purchased from Guangdong Institute of Microbiology), 0.5% Collapsing Enzyme (Driselase, purchased from FLUKA AG, CHEM);

[0042] Osmotic pressure stabilizer: 0.6mol / L mannitol;

[0043] MYG regeneration medium: 100ml of 0.6mol / L mannitol contains 10g of maltose, 4g of glucose, 4g of yeast powder, natural pH;

[0044] Liquid fermentation medium: glucose 2%, soybean meal 2%, corn flour 1.5%, KH 2 PO 4 0.1%, MgSO 4 .7H 2 O0.05%, distilled water 1000ml, pH 6.0;

[0045] Primary shake flask seed medium or secondary shake flask seed medium: glucose 1.5%, soybean meal 2%, corn flour 1.5%, bran 0.5%,...

Embodiment 2

[0070] Materials used:

[0071] Potato dextrose agar (PDA) slant medium: distilled water, 20% potato infusion, 2% glucose and 2% agar powder.

[0072] MYG liquid medium: 100ml distilled water contains 8g maltose, 4g glucose, 3g yeast powder, natural pH;

[0073] Composite enzyme: 2% by mass percentage of Lywallzyme and 0.2% of Lywallzyme mixed in a ratio of 1:1; Lywallzyme: purchased from Guangdong Institute of Microbiology; Collapsing enzyme (Driselase): purchased from From FLUKA AG, CHEM;

[0074] Osmotic pressure stabilizer: 0.8mol / L mannitol;

[0075] MYG regeneration medium: 100ml of 0.8mol / L mannitol contains 8g of maltose, 4g of glucose, 3g of yeast powder, and natural pH;

[0076] Liquid fermentation medium: glucose 1.5%, soybean meal 2.5%, corn flour 1%, KH 2 PO 4 0.05%, MgSO 4 .7H 2 O 0.05%, distilled water 1000ml, pH 6.5;

[0077] Primary shake flask seed medium or secondary shake flask seed medium: glucose 1%, soybean meal 2.5%, corn flour 1%, bran 0.2%, K...

Embodiment 3

[0102] Materials used:

[0103] Potato dextrose agar (PDA) slant medium: distilled water, 20% potato infusion, 2% glucose and 2% agar powder.

[0104] MYG liquid medium: 100ml distilled water contains 10g maltose, 2g glucose, 2g yeast powder, natural pH;

[0105] Composite enzyme: it is formed by mixing 3% Lywallzyme and 0.8% collapse enzyme by mass percent; Lywallzyme (Lywallzyme) was purchased from Guangdong Institute of Microbiology; From FLUKA AG, CHEM;

[0106] Osmotic pressure stabilizer: 0.7mol / L mannitol;

[0107] MYG regeneration medium: 100ml of 0.7mol / L mannitol contains 10g of maltose, 2g of glucose, 2g of yeast powder, natural pH;

[0108] Liquid fermentation medium: glucose 1%, soybean meal 2%, corn flour 2%, KH 2 PO 4 0.1%, MgSO 4 .7H 2 O0.05%, distilled water 1000ml, pH value 6.5;

[0109] Primary shake flask seed medium or secondary shake flask seed medium: glucose 1%, soybean meal 2%, corn flour 2%, bran 1.0%, KH 2 PO 4 0.1%, MgSO 4 .7H 2 O 0.05...

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PUM

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Abstract

The invention discloses a Hirsutella hepiali Chen et Shen fungus which can be fermented at normal temperature, the fungus in systematics is named as (Hirsutella hepiali Chen et Shen)BS-1, and is mutagenized by the following method: protoplast is extracted from Hirsutella hepiali Chen et Shen strain which is separated from the stroma of the natural Cordyceps sinensis(Berk)Sacc., and the protoplast is irradiated by a 15-60W ultraviolet lamp at a place which is 10-50cm from the ultraviolet lamp for 1-300s, inoculated on MYG reproduction culture medium and cultured in a thermotank at the temperature of 25-28 DEG C; the mutagenized and cultured strains are inoculated in fermentation medium and acclimatized at the temperature of 25-28 DEG C for ten generations, and the contents of the active components are determined, the strains which grow well and have high strain powder yield and high contents of the active components are selected, and finally the Hirsutella hepiali Chen et Shen fungus strain which can be fermented at normal temperature is obtained. The invention also discloses a mutagenesis breeding method of the fungus and a fermentation process. The Hirsutella hepiali Chen et Shen strain can rapidly grow at normal temperature with short fermentation period and low production cost, so that the product has greater market competitiveness.

Description

technical field [0001] The present invention relates to a true anamorphic Trichosporium batata of Cordyceps sinensis and its mutation breeding method and fermentation process, in particular to a normal temperature fermentable Trichosporium batata fungus and its mutation breeding method and fermentation process. Background technique [0002] Cordyceps sinensis (Berk.) Sacc. is a complex formed by fungi of the genus Cordyceps ergotaceae parasitizing in the larvae of bat moths (abbreviated as Cordyceps). It is a rare and precious medicinal fungus endemic to my country. Produced in Sichuan, Guizhou, Yunnan, Qinghai, Tibet and other 3500-5000-meter high mountains. Due to the remarkable efficacy of Cordyceps sinensis in medicine and health care, as well as the excessive collection of natural resources, the natural resources of Cordyceps sinensis are now on the verge of exhaustion. As a result, the price of Cordyceps has doubled in recent years. At present, the price of high-quali...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/14C12N15/01C12P1/02C12R1/645
Inventor 李刚
Owner JIANGXI BAISHEN PHARMA
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