Artificial culture method of cordyceps longissima sporocarp

A technology of artificial cultivation and long seat insects, applied in botany equipment and methods, horticulture, application, etc., can solve the problem of few wild Cordyceps long seat resources, etc., achieve commercial sales, simple production process, and solve the shortage of resources Effect

Inactive Publication Date: 2012-10-24
ANHUI AGRICULTURAL UNIVERSITY
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Problems solved by technology

[0005] At present, there are no reports and patents on the method of cultivating Cordyceps cereus fruiting bodies at home and abroad, and there are few wild resources of Cordyceps lenticus, so artificial cultivation of its fruiting bodies is one of the effective ways to solve the problem of developing and utilizing this resource.

Method used

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Embodiment

[0028] Trichostoma elongatum ( Hirsutella longissima ) strains can be obtained by collecting mature Cordyceps longanum in the field, collecting the ejected ascospores, and transferring them to a sterile medium to separate and purify them. The fertile part is obtained by separation and purification by means of tissue separation after surface disinfection. The used hair spores of the present invention ( Hirsutella longissima ) strains, preserved in the General Microorganism Center of China Committee for the Collection of Microbial Cultures, address: No. 3, Yard No. 1, Beichen West Road, Chaoyang District, Beijing, with a preservation number of CGMCC No.5826.

[0029] The specific cultivation operation steps of Cordyceps longum fruiting body are as follows:

[0030] A. Activation and cultivation of strains in inclined test tubes

[0031] Trichostoma elongatus ( Hirsutella longissima ) strains were spot-connected to the slant test tube medium, and cultured at 25°C for 10-15 da...

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Abstract

The invention relates to an artificial culture method of cordyceps longissima sporocarp. A strain is cordyceps longissima agamotype-hirsutella longissima. The artificial culture method of the cordyceps longissima sporocarp includes A, cant tube strain activation culture; B, liquid shake flask seed culture; C, hypha stage culture; and D, sporocarp culture. The cordyceps longissima sporocarp cultured artificially has rodlike stroma and is solitary or branching and cinnamon, length is 1-7cm, the diameter is 1-3mm, a large amount of white coremium is grown at the top end or the side face of the stroma at later stage of sporocarp culture and is compact. The artificial culture method of the cordyceps longissima sporocarp is initiated, can perform large-scale culture of the cordyceps longissima sporocarp, solves the problem that wild cordyceps longissima sources are deficient, establishes a foundation for comprehensive development of cordyceps longissima edible value and industrialized production, provides technical support, and is simple in process, low in cost, wide in raw material source, non-toxic, free of pollution and capable of performing large-scale production.

Description

technical field [0001] The invention belongs to the technical field of artificial cultivation methods of medicinal fungi, in particular to the use of Trichosporum elongatum ( Hirsutella longissima ) strains were cultured to form fruiting bodies of Cordyceps longanum. Background technique [0002] Generalized Cordyceps is a large class of entomopathogenic fungi, which includes four genera. These fungi mainly parasitize on the fruiting bodies of some underground species of insects, spiders and certain macrocysts, and use the nutrition of the host to complete their activities. The life cycle of the insect-bacteria complex. It is a type of polymorphic fungi that have an asexual stage that produces conidia and a sexual stage that produces ascospores in its life cycle. Cordyceps fungi contain a variety of biologically active substances, which have the functions of antibacterial, antiviral, antitumor, antipathogenic, antioxidative, antiradiation, insecticidal, calcium ion antagon...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01G1/04
Inventor 李春如樊美珍刘洋潘丹丹丁海燕
Owner ANHUI AGRICULTURAL UNIVERSITY
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