Method of tissue culture and rapid propagation of viola prionantha
A technology of tissue culture and viola, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of few resources, and the tissue culture of early blooming viola has not been reported yet, and achieve a large reproduction coefficient and sustainable The effect of using
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[0025] The present invention will be described in detail below in conjunction with specific embodiments.
[0026] 1.2.1 Medium
[0027] (1) Configuration of culture medium
[0028] Medium formula: minimal medium (MS) supplemented with different types and concentrations of auxins and cytokinins.
[0029] The sucrose concentration is 25g / L, the agar is 6g / L, the pH value is adjusted to 6.0-6.2 with 1mol / L HCl or 1mol / L NaOH, and autoclaved at 121°C for 20min before use.
[0030] (2) Culture conditions
[0031] The culture temperature is (25±1)°C, and the light intensity is 20-30 umol / m 2 ·s, the photoperiod of 16h in light and 8h in dark was adopted for the induction, proliferation and callus differentiation of clustered shoots. The callus was induced and proliferated by daily dark culture for 24 hours, and after 7 days of culture, it was transferred to the above-mentioned photoperiod of daily light culture for 12 hours and dark culture for 12 hours.
[0032] 1.2.2 Main rea...
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