Method of tissue culture and rapid propagation of viola prionantha

A technology of tissue culture and viola, applied in horticultural methods, botanical equipment and methods, cultivation, etc., can solve the problems of few resources, and the tissue culture of early blooming viola has not been reported yet, and achieve a large reproduction coefficient and sustainable The effect of using

Inactive Publication Date: 2013-04-03
SICHUAN AGRI UNIV
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] As a wild ground cover plant, Viola early blooming has not yet been developed. The source of medicine is mainly to dig wild resources, and the resources are gradually decreasing. At present, the tissue culture of Viola early blooming has not been reported, and relying solely on wild resources cannot meet the needs.

Method used

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  • Method of tissue culture and rapid propagation of viola prionantha
  • Method of tissue culture and rapid propagation of viola prionantha
  • Method of tissue culture and rapid propagation of viola prionantha

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Embodiment Construction

[0025] The present invention will be described in detail below in conjunction with specific embodiments.

[0026] 1.2.1 Medium

[0027] (1) Configuration of culture medium

[0028] Medium formula: minimal medium (MS) supplemented with different types and concentrations of auxins and cytokinins.

[0029] The sucrose concentration is 25g / L, the agar is 6g / L, the pH value is adjusted to 6.0-6.2 with 1mol / L HCl or 1mol / L NaOH, and autoclaved at 121°C for 20min before use.

[0030] (2) Culture conditions

[0031] The culture temperature is (25±1)°C, and the light intensity is 20-30 umol / m 2 ·s, the photoperiod of 16h in light and 8h in dark was adopted for the induction, proliferation and callus differentiation of clustered shoots. The callus was induced and proliferated by daily dark culture for 24 hours, and after 7 days of culture, it was transferred to the above-mentioned photoperiod of daily light culture for 12 hours and dark culture for 12 hours.

[0032] 1.2.2 Main rea...

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Abstract

The invention discloses a method of tissue culture and rapid propagation of viola prionantha. The method comprises: (1) a viola prionantha explant best sterilization way; (2) primary culture; (3) subculture; (4) rooting culture; and (5) hardening-seedling and transplantation. The viola prionantha cultured by the method has a great propagation coefficient, is suitable large-scale production, can solve the problem of scanty resource of the viola prionantha and realize sustainable utilization; in addition, the viola prionantha is propagated rapidly, and a large number of nursery stocks having consistent specification can be obtained in a short time, so that the method of tissue culture and rapid propagation of viola prionantha can be satisfied with the requirements of landscaping on great quantity demand of the viola prionantha, and is of great significance to gardens and medicines.

Description

technical field [0001] The invention relates to a method for plant tissue culture and rapid propagation, and belongs to the technical field of plant tissue culture industrialized rapid seedling growth methods. Background technique [0002] Viola prionantha Bunge is a perennial herbaceous plant of the genus Viola in the Viola family. It turns green early, grows rapidly, has a strong ability to cover the ground, and has a concentrated flowering period. It can bloom in spring and autumn within a year, and has a strong ability to suppress weeds. As a wild flowering ground cover plant in early spring, it has good ornamental effect and garden application value. [0003] At the same time, viola viola has high medicinal value. It is widely used in folks for carbuncle sores and poisonous snake bites. It has strong antibacterial, anti-inflammatory, heat-clearing and detoxifying effects. [0004] Early-opening Viola mainly propagates by seeds and rhizomes, but the reproduction rate of...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): A01H4/00A01G31/00
Inventor 张帆万雪琴刘敏钟宇刘光立张双罗兵黄金亮吴富雨
Owner SICHUAN AGRI UNIV
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