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Recycling method of hirsutella sinensis fermentation filtrate

A Chinese Trichospora, fermentation broth technology, applied in microorganism-based methods, fermentation, biochemical equipment and methods, etc., can solve problems such as scaling of electrodialysis equipment, unsatisfactory desalination efficiency, reverse osmosis, etc.

Active Publication Date: 2016-06-01
ZHEJIANG UNIV OF TECH +2
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In addition, the pretreatment (filtration, etc.) of the fermentation filtrate before electrodialysis requires a certain amount of cost, and the difference in concentration is likely to cause fouling of the electrodialysis equipment and reverse osmosis, resulting in unsatisfactory desalination efficiency. Therefore, electrodialysis is rarely used. used by pharmaceutical companies

Method used

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  • Recycling method of hirsutella sinensis fermentation filtrate
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  • Recycling method of hirsutella sinensis fermentation filtrate

Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0035] Example 1: Cultivation of "Bailing" production fungus Cordyceps sinensis

[0036] Source of the strain: Firstly, natural Cordyceps sinensis was collected from Qinghai, and brought back to Hangzhou for isolation and screening, and the L0106 strain was obtained, which was identified as Hirsutellasinensis by strain identification, and the strain was preserved in the Chinese Type Culture Collection The deposit center, the deposit number is CCTCCNo: M2011278, which has been disclosed in the previously applied patent CN102373190A.

[0037] This bacterial classification is inoculated on the slant, and the culture medium formula (this is the liquid formula before solidification, makes the slant after being prepared according to the following ratio) is: glucose 2.0% (w / v, 1% means that 100mL culture medium contains 1g, the same below), corn flour 1.0%, potato juice 0.5% (the potato juice refers to the filtrate obtained by boiling 200g / L potatoes with water and filtering through ...

Embodiment 2

[0038] Example 2: Detection and analysis of active components in Mortierella chinensis fermentation filtrate

[0039] 1. Preparation of Fermentation Filtrate of Mortierella chinensis

[0040] Example 1 After the fermentation is finished, the fermentation liquid is put into a tank for separation, and the fermentation filtrate of the present invention is obtained after separation by a plate and frame filter press. The obtained fermentation filtrate is brown yellow transparent liquid. The pH is 6.0-7.0.

[0041] 2. Detection and analysis of active components in fermentation filtrate

[0042]Accurately draw 0.2mL of the isolated Mortierella sinensis fermentation filtrate, dilute to 20mL with ultrapure water, mix well, take 1mL into a clean test tube, add 1mL potassium periodate solution at the same time, react at 25°C for 10min, then add 0.1 2 mL of wt% L-rhamnose aqueous solution was shaken evenly, and 4 mL of freshly prepared Nash solution was added, and the color was develop...

Embodiment 3

[0048] Example 3: Preparation of Mortierella chinensis fermentation filtrate

[0049] Put 78 tons of Cordyceps sinensis cultured by the method of Example 1 into a 6-ton high-level storage tank, and use the pressure of the potential difference to squeeze the fermented liquid into the drum at a speed of 10 tons / hour by controlling the valve. , use the drum to separate the solid and liquid of the fermentation broth, and obtain about 75 tons of fermentation filtrate. During the experiment, the fermentation filtrate stock solution, the fermentation filtrate obtained by centrifugation after activated carbon decolorization, and the fresh water of the fermentation filtrate after electrodialysis were collected respectively (for comparing the recycling effect, distilled water was used as the experimental control).

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Abstract

The invention provides a recycling method of hirsutella sinensis fermentation filtrate. The method includes: using the filtrate obtained by filtering the fermentation broth acquired by the fermentation culture of hirsutella sinensis as inlet water, performing electrodialysis treatment under a condition that water inlet flow is 200-400L / h and the operation voltage is 20-30V, collecting electrodialysis effluent liquid, namely the recycled water of the hirsutella sinensis fermentation filtrate, and using the recycled water to prepare microorganism culture media. The recycling method has the advantages that the electrodialysis equipment selected by the method is cheaper than imported equipment, and the salt rejection rate of the electrodialysis equipment can reach more than 95% under corresponding parameter setting; the water yield after the electrodialysis is about 90%; when the electrodialysis method is used to treat 1000L of fermentation filtrate, the energy consumption cost of the electrodialysis method is about 1.88 yuan per ton, and the equipment cost is about 0.5 yuan per ton; enterprise production cost can be evidently lowered when the electrodialysis method is used to treat the fermentation filtrate and the recycled water is used for preparing the culture media.

Description

(1) Technical field [0001] The invention relates to a recycling method of the fermented filtrate of the Cordyceps sinensis producing fungus Mortierella sinensis and a circular application of the filtrate after electrodialysis. (2) Background technology [0002] Cordyceps sinensis (Cordyceps sinensis (Berk.) Sacc.) is a complex (including the sub-base and worm body) that Cordyceps sinensis parasitizes on the larvae of Hepialus armoricanus Oberthur (Lepidoptera) larvae. Cordyceps sinensis is a kind of precious traditional fungal medicinal resources, which has the characteristics of diverse metabolites and biological activities, and has shown great application and development prospects in the field of biomedicine. [0003] In the 1980s, Hangzhou Zhongmei Huadong Pharmaceutical Co., Ltd., one of the inventors of the present invention, completed the research on the artificial production of Cordyceps sinensis, which is to carry out microbial liquid fermentation culture of the fung...

Claims

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Application Information

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IPC IPC(8): C12P1/02C12N1/20C12N1/14C12R1/645
CPCC12N1/14C12N1/20C12N2500/74C12P1/02C12N1/145C12R2001/645
Inventor 柳志强郑裕国林善薛亚平吴晖李邦良许峰
Owner ZHEJIANG UNIV OF TECH
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