Toxoplasma gondii aptamer sequence and use of toxoplasma gondii aptamer sequence in preparing toxoplasmosis diagnostic reagent or product

A nucleic acid aptamer, toxoplasmosis technology, applied in the fields of biomedicine and clinical medicine, can solve the problems of inability to accurately distinguish chronic infection from acute infection, inability to distinguish acute and chronic infection, etc., achieve high specificity, high affinity, High stability effect

Inactive Publication Date: 2016-08-17
张阳
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Problems solved by technology

2. IgG antibodies usually appear 1 to 2 weeks after infection, reach a peak after 1 to 2 months, then gradually decrease, and can remain positive throughout life. Therefore, IgG positivity cannot distinguish between acute and chronic infections
It can be seen that positive T. gondii IgM and IgG results obtained in non-reference laboratories cannot accurately distinguish chronic infection from acute infection

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  • Toxoplasma gondii aptamer sequence and use of toxoplasma gondii aptamer sequence in preparing toxoplasmosis diagnostic reagent or product
  • Toxoplasma gondii aptamer sequence and use of toxoplasma gondii aptamer sequence in preparing toxoplasmosis diagnostic reagent or product
  • Toxoplasma gondii aptamer sequence and use of toxoplasma gondii aptamer sequence in preparing toxoplasmosis diagnostic reagent or product

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[0025] The present invention will be described in detail below in conjunction with the accompanying drawings, in order to further illustrate the present invention, rather than limit the present invention.

[0026] The nucleic acid aptamers YZ-39 and YZ-56 are based on a SELEX method, using the Toxoplasma gondii rod-shaped secreted protein ROP-GST as the screening object, and the GST tag protein as the reverse screening object. After 15 rounds of screening, A nucleic acid aptamer that can specifically bind to the secretory protein ROP of the rod-like body of Toxoplasma gondii. YZ-39 and YZ-56 were synthesized by DNA synthesizer according to their sequence order, then purified by high performance liquid chromatography (HPLC) or denaturing electrophoresis (ULTRAPAGE), and finally prepared by centrifugal concentration and drying.

[0027] Preferably, in order to clarify the specific binding of the screened nucleic acid aptamers YZ-39 and YZ-56 to the rod-shaped secreted protein of...

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Abstract

The invention relates to a method for detecting and diagnosing early-stage acute infection of toxoplasmosis by utilizing aptamers which are separately YZ-39 and YZ-56. The action mechanism of the aptamers is that the aptamers are specifically combined with a rhoptry secretory protein ROP of the toxoplasma gondii. The secretory protein ROP is the early-stage secretory protein of a toxoplasma gondii infected host cell, and is of great significance on diagnosing early-stage acute infection of the toxoplasmosis. At present, the report about detecting early-stage acute infection of toxoplasmosis by utilizing aptamers to detect the secretory protein ROP is not available at home and abroad. The discovery and application of the ROP specific aptamers provide a novel method for detecting the early-stage acute infection of the toxoplasmosis.

Description

technical field [0001] The invention belongs to the technical field of biomedicine and clinical medicine, and relates to the use of a nucleic acid aptamer related to toxoplasma gondii in reagents or products for the diagnosis and detection of early acute infection of toxoplasmosis. Background technique [0002] Since the invention of monoclonal antibodies produced by hybridoma technology, antibodies have made great contributions to the development of medical research, clinical diagnosis and biological therapy. Nucleic acid aptamers can not only bind to target molecules efficiently and specifically like antibodies, but also have many advantages that antibodies cannot match, such as small molecular weight, strong self-stability, fast and reversible denaturation and refolding, easy modification and labeling, etc. The recognition and detection method based on nucleic acid aptamer is gradually becoming a new and universally applicable technology, providing an efficient and rapid ...

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N15/115G01N33/569
CPCC12N15/115C12N2310/16G01N33/56905G01N2333/45
Inventor 张阳
Owner 张阳
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