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The technology of chamomile and seeds is applied in the field of plant tissue culture, and can solve the problems of unfavorable large-scale chamomile, industrialized production and cultivation, low callus induction rate and adventitious bud differentiation rate, and failure to obtain a chamomile regeneration system, To achieve the effect of promoting callus growth, good growth state and consistent size
Inactive Publication Date: 2012-01-04
BEIJING FORESTRY UNIVERSITY
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Problems solved by technology
Although there are many studies on the regeneration system of chamomile, so far, no stable regeneration system of chamomile has been obtained.
In the prior art, although chamomile leaves can be used as explants to establish the regeneration system of chamomile, the regeneration time is longer, the callus induction rate and the differentiation rate of adventitious buds are low, and the regeneration system is not stable enough. Poor resistance, which is not conducive to the large-scale, factory production and cultivation of chamomile
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Embodiment 1
[0052] 1. Test materials
[0053] 1. Camomile seeds: collected from the campus of Beijing Forestry University in the autumn of 2009. The planting plants grew well, the seeds were mature and full, and the seed coats were intact.
[0054] 2. Plant growth regulator
[0055] The plant growth regulator used in the present invention adopts domestic naphthalene acetic acid (NAA), 6-benzylamino adenine (6-BA) and 2,4-dichlorophenoxyacetic acid (2,4-D).
[0056] 3. Preparation of culture medium:
[0057] (1) The composition or preparation method of "MS basic medium";
[0058] Table 1 MS medium (Murashige and Skoog, 1962)
[0059]
[0060] After the above MS medium stock solution is prepared, it should be stored in a refrigerator at 4°C until use. According to the quantity of the preparation medium, weigh the required agar and sucrose, pour them into sterile water of 3 / 4 of the volume of the medium to be prepared, and sequentially add the required macroelement mother liquor, trace element mother l...
Embodiment 2
[0092] The culture time is 5 days except for sterile seedlings; 2,4-D 0.5mg / L, 6-BA 0.1mg / L used in callus induction medium; NAA 0.2mg / L used in rooting medium; light for adventitious bud differentiation culture Except for the period of 14 hours of light / 10 hours of darkness, the others are the same as in Example 1.
Embodiment 3
[0094] Except for aseptic seedlings, the culture time is 6 days; the callus induction medium uses 2,4-D 1.0mg / L, 6-BA 1.0mg / L; the adventitious bud differentiation culture light cycle is 14 hours of light / 10 hours of darkness; The rooting medium used NAA 0.3 mg / L, and the photoperiod was 14 hours of light / 10 hours of darkness, and everything else was the same as in Example 1.
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Abstract
The invention discloses a method for breeding chamomile. The method disclosed by the invention comprises the following steps: (1) inoculating chamomile seeds onto a seed germination culture medium to carry out sterile seedling cultivation so as to produce sterile seedlings; (2) taking hypocotyls of the sterile seedlings as explants, inoculating the explants onto a callus induction culture medium to carry out callus induction culture so as to generate calluses by virtue of induction; (3) inoculating the calluses generated by induction onto an adventitious bud differentiation culture medium to carry out adventitious bud induction differentiation culture so as to obtain adventitious buds; (4) inoculating the adventitious buds onto a rooting culture medium to carry out rooting culture and culturing adventitious roots by virtue of induction so as to obtain rooted seedlings; and (5) hardening and transplanting the rooted seedlings so as to finally obtain the chamomile. In a regeneration system established by the method disclosed by the invention, callus inductivity reaches up to 86.63%, differentiation rate of the adventitious buds reaches up to 25.5%, rooting rate reaches up to 100%, and transplanting survival rate reaches up to 100%, thus a large number of excellent chamomile test-tube plantlets can be obtained in short term so as to realize large-scale factory production.
Description
Technical field [0001] The invention relates to a method for plant tissue culture, in particular to a method for in vitro culture and plant regeneration of chamomile, belonging to the field of plant tissue culture. Background technique [0002] Chrysanthemum lavandulifolium (Chrysanthemum lavandulifolium), also known as rock chrysanthemum and chamomile, is a perennial herbaceous plant belonging to the chamomile family of the Compositae family. It is one of the important parents of Chrysanthemum×morifolium. Ornamental plants. It is distributed in many provinces and cities in our country, mostly in hillsides, rocks, river valleys, river banks, wastelands and loess hilly areas at an altitude of 630-2300 meters. [0003] Camomile is a good water-saving wild ground cover plant material. It has the advantages of strong resistance, wide application range and extensive management. It is suitable for green hillsides, road slope protection, river embankment planting, and can prevent soil er...
Claims
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