A method for inducing loose callus of Dracaena glabra
A technology of callus induction and Dracaena glabrata, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of cell suspension culture, etc., and achieve low price, fast growth, and low test cost Effect
- Summary
- Abstract
- Description
- Claims
- Application Information
AI Technical Summary
Problems solved by technology
Method used
Examples
Embodiment 1
[0017] An example of the method for the induction of the loose callus of Dracaena glabrata of the present invention, comprises the steps:
[0018] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;
[0019] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a...
Embodiment 2
[0023] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;
[0024] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a culture temperature of 25±2°C, with a light intensity of 20-30 μmol / m 2 s, cultivated under the condition of light time of 12h / d, and sprou...
Embodiment 3
[0028] (1) Disinfection of explants: first clean the dirt on the surface of the explants with an aqueous solution of detergent, rinse them with running water for 5 to 8 minutes in a beaker, then move them to an ultra-clean workbench, and decontaminate the seeds with 75v / v% alcohol. Soak with buds for 30s, rinse with sterile water once, and then use 0.1v / v% HgC1 2 Soak for 8, 12, and 15 minutes respectively, and soak in sterile water for 3 times. Blot dry the surface moisture of the explants with sterile filter paper and inoculate them, wherein the sterile water is distilled water sterilized by high temperature and high pressure; the explants are plump seeds or buds germinated from seeds sown in river sand;
[0029] (2) Acquisition of sterile seedlings: Inoculate the explants obtained in step (1) into MS induction medium respectively, at a culture temperature of 25±2°C, with a light intensity of 20-30 μmol / m 2 s, cultivated under the condition of light time of 12h / d, and sprou...
PUM
Abstract
Description
Claims
Application Information
- R&D Engineer
- R&D Manager
- IP Professional
- Industry Leading Data Capabilities
- Powerful AI technology
- Patent DNA Extraction
Browse by: Latest US Patents, China's latest patents, Technical Efficacy Thesaurus, Application Domain, Technology Topic, Popular Technical Reports.
© 2024 PatSnap. All rights reserved.Legal|Privacy policy|Modern Slavery Act Transparency Statement|Sitemap|About US| Contact US: help@patsnap.com