Method for tissue culture and rapid propagation of oxalis triangularis
A rapid propagation technology of purple leaf sorrel and tissue culture, applied in horticultural methods, botanical equipment and methods, horticulture, etc., can solve the problems of slow propagation speed of ramets, high labor cost, slow propagation speed, etc., and achieve the applicable The effect of wide range, improved efficiency, and short incubation time
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Embodiment 1
[0028] A kind of tissue culture rapid propagation method of Oxalis oleracea described in the present embodiment, comprises the following steps:
[0029] (1) Selection and sterilization of explants: select the leaves or petioles of Oxalis oleifera, wash them with tap water first, then rinse them with running water for 30 minutes, treat them with 75% alcohol for 20-40 seconds, and then use 0.1% Mercury treatment for 3-4 minutes, rinse with sterile water for 4-6 times, absorb water with absorbent paper, cut petioles into 0.5-1.5 cm length, or cut leaves into squares with side length of 0.5-1.5 cm to obtain explants ;
[0030] (2) Primary culture: the explants obtained in step (1) are inoculated into the primary medium with a pH value of 5.8-6.0 and cultivated for 5-6 weeks, and the seedlings are generated. The composition of the primary medium is: MS basic medium , supplemented with 1.0mg / L6-BA, 0.2mg / LNAA, 0.1mg / LIAA, 30g / L sucrose, 5.5g / L agar;
[0031] (3) Proliferation cult...
Embodiment 2
[0037] The tissue culture rapid propagation method of a kind of purple leaf sorrel described in the present embodiment, the difference with embodiment 1 is:
[0038] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 0.5mg / L6-BA, 0.1mg / LNAA, 0.1mg / LIAA, 30g / L sucrose, and 5.5g / L agar;
[0039] In step (3), the composition of the proliferation medium is: MS basic medium supplemented with 0.5mg / L6-BA and 0.2mg / LNAA;
[0040] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium, supplemented with 0.1 mg / L NAA, 30 g / L sucrose, and 5.5 g / L agar.
[0041] In the present embodiment, in the primary culture process, the first seedling generation time was 45 days, and when the primary culture ended, the seedlings elongated by 2-3 cm; during the proliferation culture process, the first clustered buds generated time was 23 days, At the end of the multiplication culture, the height of the clustered buds was 5 cm, and the multiplic...
Embodiment 3
[0043] The tissue culture rapid propagation method of a kind of purple leaf sorrel described in the present embodiment, the difference with embodiment 1 is:
[0044] In step (2), the composition of the primary medium is: MS basic medium, supplemented with 1.0mg / L6-BA, 0.3mg / LNAA, 0.2mg / LIAA, 30g / L sucrose, 5.5g / L agar;
[0045] In step (3), the composition of the proliferation medium is: MS basic medium supplemented with 1.0mg / L6-BA and 0.4mg / LNAA;
[0046] In step (4), the composition of the rooting medium is: 1 / 2 MS basic medium supplemented with 0.05 mg / L NAA, 20 g / L sucrose, and 5.5 g / L agar.
[0047] In the present embodiment, in the primary culture process, the first seedling generation time was 35 days, and when the primary culture ended, the seedlings elongated by 2-3 cm; during the proliferation culture process, the first clustered buds generated time was 20 days, At the end of the multiplication culture, the height of the clustered buds was 5 cm, and the multiplicat...
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