Method for breeding chamomile

A chamomile and seed technology, applied in the field of plant tissue culture, can solve the problems of unfavorable chamomile scale, factory production and cultivation, callus induction rate, low rate of adventitious bud differentiation, chamomile regeneration system not obtained, etc. Achieve the effect of promoting callus growth, good growth state, and consistent size
CN102301952BInactive Publication Date: 2012-11-28BEIJING FORESTRY UNIVERSITY
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Patents(China)
Current Assignee / Owner
BEIJING FORESTRY UNIVERSITY
Publication Date
2012-11-28
Estimated Expiration
Not applicable · inactive patent

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Abstract

The invention discloses a method for breeding chamomile. The method disclosed by the invention comprises the following steps: (1) inoculating chamomile seeds onto a seed germination culture medium to carry out sterile seedling cultivation so as to produce sterile seedlings; (2) taking hypocotyls of the sterile seedlings as explants, inoculating the explants onto a callus induction culture medium to carry out callus induction culture so as to generate calluses by virtue of induction; (3) inoculating the calluses generated by induction onto an adventitious bud differentiation culture medium to carry out adventitious bud induction differentiation culture so as to obtain adventitious buds; (4) inoculating the adventitious buds onto a rooting culture medium to carry out rooting culture and culturing adventitious roots by virtue of induction so as to obtain rooted seedlings; and (5) hardening and transplanting the rooted seedlings so as to finally obtain the chamomile. In a regeneration system established by the method disclosed by the invention, callus inductivity reaches up to 86.63%, differentiation rate of the adventitious buds reaches up to 25.5%, rooting rate reaches up to 100%, and transplanting survival rate reaches up to 100%, thus a large number of excellent chamomile test-tube plantlets can be obtained in short term so as to realize large-scale factory production.
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Description

technical field

[0001] The invention relates to a plant tissue culture method, in particular to a chamomile in vitro culture and plant regeneration method, and belongs to the field of plant tissue culture. Background technique

[0002] Chamomile (Chrysanthemum lavandulifolium), also known as rock chrysanthemum, chamomile, is a perennial herb of the family Asteraceae and is one of the important parents of the origin of chrysanthemum (Chrysanthemum×morifolium). It has good ornamental value and belongs to wild ornamental plants. It is distributed in many provinces and cities in my country, mostly in hillsides, rocks, river valleys, river banks, wasteland and loess hilly areas at an altitude of 630-2300 meters.

[0003] Chamomile is a good water-saving wild ground cover plant material. It has the advantages of strong stress resistance, wide application range, and extensive management. It is suitable for hillside greening, road slope protection, and river embankment planting, an...

Claims

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