Microbial strain and deodorant for municipal landfill, and preparation and application method thereof
A municipal solid waste and application method technology, applied in the field of microbial deodorant, can solve the problems of continuous product quality and use effect, unstable proportion of effective bacteria group, high production input and product cost, etc., to reduce the probability of bacterial contamination, Good deodorizing effect, shortening the fermentation cycle
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Embodiment 1
[0033] Prepare PDA medium and MRS medium, and sterilize at 121°C for 15 minutes. Subsequently, PDA was inoculated with Saccharomyces cerevisiae CGMCC No.5183, and cultured for 20 h at a culture temperature of 28° C. to 30° C. and a rotation speed of 180 rpm to obtain a yeast seed liquid. Lactobacillus casei CGMCC No.5182 was inoculated in MRS medium, and cultured at 35° C. to 37° C. for 30 hours to obtain Lactobacillus seed liquid.
[0034] Preparation of fermentation medium: 10% molasses, 3% bean sprout juice, 1% wheat bran, 0.1% yeast extract, 0.1% peptone, the rest is water, pH 5.0-5.5; sterilize in a fermenter at 121°C for 15 minutes ;
[0035] Insert Saccharomyces cerevisiae seed liquid accounting for 1% of the volume of the medium into the sterilized fermentation medium that accounts for 50% to 70% of the volume of the fermentation tank. The culture temperature is 28°C, the tank pressure is 0.01 to 0.03MPa, and the air is first ventilated. Stirring, the ratio of aerati...
Embodiment 2
[0040] Prepare PDA medium and MRS medium, and sterilize at 121°C for 15 minutes. Subsequently, Saccharomyces cerevisiae was inoculated into the PDA, and cultured for 20 hours at a culture temperature of 28° C. to 30° C. and a rotation speed of 180 rpm to obtain a yeast seed liquid. Lactobacillus casei was inoculated in MRS medium, and cultured at 35°C to 37°C for 30 hours to obtain Lactobacillus seed liquid.
[0041] Preparation of fermentation medium: 10% molasses, 3% bean sprout juice, 1% wheat bran, 0.1% yeast extract, 0.1% peptone, the rest is water, pH 5.0-5.5; sterilize in a fermenter at 121°C for 15 minutes .
[0042] Insert Saccharomyces cerevisiae seed liquid accounting for 2% of the volume of the medium into the sterilized fermentation medium that accounts for 50% to 70% of the volume of the fermentation tank. The culture temperature is 30°C, the tank pressure is 0.01-0.03MPa, and the air is passed first. Stirring, the ratio of aeration volume to fermentation broth...
Embodiment 3
[0047] Prepare PDA medium and MRS medium, and sterilize at 121°C for 15 minutes. Subsequently, Saccharomyces cerevisiae was inoculated into the PDA, and cultured for 20 hours at a culture temperature of 28° C. to 30° C. and a rotation speed of 180 rpm to obtain a yeast seed liquid. Lactobacillus casei was inoculated in MRS medium, and cultured at 35°C to 37°C for 30 hours to obtain Lactobacillus seed liquid.
[0048] Preparation of fermentation medium: 10% molasses, 3% bean sprout juice, 1% wheat bran, 0.1% yeast extract, 0.1% peptone, the rest is water, pH 5.0-5.5; sterilize in a fermenter at 121°C for 15 minutes .
[0049] Insert Saccharomyces cerevisiae seed liquid accounting for 3% of the volume of the medium into the sterilized fermentation medium that accounts for 50% to 70% of the volume of the fermentation tank. The culture temperature is 32°C, the tank pressure is 0.01 to 0.03MPa, and the air is passed first. Stirring, the ratio of aeration volume to fermentation br...
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