Lactobacillus reuteri and application thereof

A technology of Lactobacillus reuteri and Lactobacillus reuteri, applied in Lactobacillus reuteri and its application field, can solve the problems of insufficient bile salt resistance and loss

Pending Publication Date: 2015-11-18
BEIJING BOJINYUAN BIOTECH
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

However, when the bacteria are not resistant to acid and bile salts, they cannot enter the intestinal tract and lose their function

Method used

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  • Lactobacillus reuteri and application thereof
  • Lactobacillus reuteri and application thereof
  • Lactobacillus reuteri and application thereof

Examples

Experimental program
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Embodiment Construction

[0023] An example is: this Lactobacillus reuteri, it is Lactobacillus reuteri LZ262, the preservation number is CGMCCNo.11240, the preservation date is August 14, 2015, the preservation unit: China Microorganism Culture Collection Management Committee General Microbiology Center.

[0024] The isolation and identification methods of Lactobacillus reuteri LZ262 are as follows:

[0025] 1. Separation method

[0026] Weigh 5g of the sample (healthy pig intestine) into 45ml sterile buffered saline solution (add an appropriate amount of glass beads) as the mother solution, shake for 10 minutes and mix well, make gradient dilution with sterile buffered saline solution, select three to five suitable Gradient, pipette 0.1 ml into MRS medium for coating separation, and do two replicates for each dilution gradient. The petri dish was placed in a 37°C incubator for 48-72 hours, and then smooth, convex gardens, neat edges, white or milky white colonies were selected for microscopic exami...

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Abstract

The invention relates to lactobacillus reuteri and application thereof. Lactobacillus reuteri is lactobacillus reuteri LZ262 which is preserved in the Common Microorganism Center of the China Committee for Culture Collection of Microorganisms on 13 August, 2015 with the preservation number of CGMCCNo.11240. Lactobacillus reuteri LZ262 is from the intestinal tract of a healthy pig, can be colonized in a human body and an animal body, and has higher tolerance to gastric acid and bile salt; in addition, lactobacillus reuteri LZ262 has a better suppression effect on Escherichia coli, and has a higher survival rate after spray-drying, and higher fermentation density is achieved by improving a fermentation formula; the strain has a better effect of synergistic growth with other types of lactic acid bacteria, can be used for food and animal feed additives, and can also be widely applied to other food and biological products.

Description

technical field [0001] The invention relates to the field of biological preparations, in particular to a Lactobacillus reuteri and application thereof. Background technique [0002] Lactobacillus reuteri, also known as Lactobacillus reuteri, is closely related to the life of humans and animals. It is a lactic acid bacteria commonly found in the intestinal tract of humans, pigs, poultry and other animals. It can pass through the stomach and colonize the intestinal tract to exert beneficial effects. For example, it has various functions such as maintaining the balance of intestinal flora, promoting nutrient absorption, relieving lactose intolerance, and inhibiting the formation of tumor cells. Currently, the application fields with more research reports include food, feed, medical care, and cosmetics. , beauty, aquaculture, industrial and agricultural production, biological preservatives, probiotic preparations, etc. [0003] The various physiological functions of Lactobacill...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12N1/20A23K1/16A23K1/18C12R1/225
Inventor 姜波姜瑾刘英华刘金华郝应海
Owner BEIJING BOJINYUAN BIOTECH
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