Culture mediums for producing doramectin through fermentation of mutant streptomyces avermitilis and material supplement method

Abstract

The invention relates to culture mediums for producing doramectin through fermentation of mutant streptomyces avermitilis and a material supplement method. The culture mediums comprise a seed culture medium and a fermentation culture medium, and are characterized in that the seed culture medium comprises corn lees and low-temperature pressing soya bean cake powder; the fermentation culture medium comprises corn lees, low-temperature pressing soya bean cake powder, polystyrene non-polar adsorbent resins and nonionic surfactants. The cost problem of raw materials and auxiliary materials are solved, the environment influence on sources of the raw materials and the auxiliary materials is reduced to the hilt, the supplying of the raw materials and the auxiliary materials is sufficient, the stable and efficient production of the doramectin is realized, and at the same time, the fermentation unit can be improved by using the culture mediums and the material supplement method.

Description

technical field [0001] The invention belongs to the field of fermentation technology, and in particular relates to a culture medium and feeding method for doramectin fermented by mutant Streptomyces avermitilis. Background technique [0002] Doramectin is a new type of macrolide antiparasitic drug, which is biosynthesized by mutant Streptomyces avermitilis in a fermentation mode. In comparison, its anti-parasitic range is wider, its insecticidal activity is higher, and its effective time for preventing parasitic re-infection is longer. It is considered to be the most promising new veterinary anti-parasitic drug. [0003] At present, the domestic use of mutant Streptomyces avermitilis to produce doramectin by fermentation, the main problems are: [0004] 1 The fermentation production process has not changed much, and the fermentation technology level is maintained at 1000-1200u / ml, resulting in a low overall fermentation yield. [0005] 2 The quality of animal nitrogen sour...

AI Technical Summary

Problems solved by technology

[0004] 1 The fermentation production process has not changed much, and the fermentation technology level is maintained at 1000-1200u / ml, resulting in a low overall fermentation yield

[0005] 2 The quality of animal nitrogen source affects the quality and fermentation effect of fermentation broth

At present, peptone or yeast extract is added to the medium for domestic fermentation production of doramectin, or two or more nitrogen sources are selected to be added at the same time, resulting in more residual protein in the late stage of fermentation and higher viscosity of the fermentation broth, which reduces the extraction yield. Rate

[0006] 3 The available carbon source in the conventional medium is glucose, and part of the glucose is carbonized during the sterilization process of the medium, which affects the quality of the fermentation broth

[0007] 4. The production cost of doramectin is relatively high, and the cost of nitrogen source accounts for about 30% of the fermentation cost