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Candida tropicalis and application thereof, and production method for long chain diprotic acid

A Candida, long-chain dibasic acid technology, applied in the field of fermentation, can solve problems such as affecting the development of the biological method long-chain dibasic acid industry, slow metabolism of strains, and inability to carry out fermentation, and achieves obvious industrialized value advantages and simplification. Extraction process, the effect of reducing pressure on resources and the environment

Pending Publication Date: 2019-09-10
CATHAY R&D CENT CO LTD +1
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

In the fermentation broth after fermentation, the long-chain dibasic acid exists in the fermentation broth in the form of salt, and a large amount of acid needs to be added in the process of extracting and purifying the dibasic acid to convert the salt of the long-chain dibasic acid into Long-chain dibasic acid products cause the concentration of salt in the fermentation treatment liquid system to be as high as 50,000-70,000ug / ml. These high-salt wastewater is difficult to treat, which is a very important challenge to the environment and seriously affects the biological method of long-chain dibasic acids. industry development
[0004] Patent US 6569670B2 reports a method for producing long-chain dibasic acids using fatty acids as raw materials in an environment with a pH value of 5.8. The main reason is that fatty acids exist in the form of fatty acid salts when the pH value is above 7.0, which is particularly prone to foaming. As a result, the fermentation cannot be carried out, so the fermentation must be carried out at a lower pH value, but the metabolism of the strain at a low pH value is slow and the production efficiency is low

Method used

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  • Candida tropicalis and application thereof, and production method for long chain diprotic acid
  • Candida tropicalis and application thereof, and production method for long chain diprotic acid
  • Candida tropicalis and application thereof, and production method for long chain diprotic acid

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preparation example Construction

[0068] A fifth aspect of the present invention provides a method for preparing a long-chain dibasic acid, comprising the following steps:

[0069] S1: fermentation to prepare long-chain dibasic acid fermentation broth; and

[0070] S2: extracting and purifying the obtained long-chain dibasic acid fermentation broth to obtain the long-chain dibasic acid;

[0071] Wherein, step S1 adopts the method for producing long-chain dibasic acid by fermentation according to the fourth aspect of the present invention to prepare the long-chain dibasic acid fermentation broth.

[0072] In the preparation method of the long-chain dibasic acid of the present invention, the obtained long-chain dibasic acid fermentation broth can be obtained by using the existing extraction and purification process to obtain the final long-chain dibasic acid product.

[0073] In one embodiment of the method for preparing long-chain dibasic acids according to the present invention, the extraction and purificatio...

Embodiment 1

[0088] Example 1 The acquisition of Candida tropicalis CAT H1614 strain

[0089] Connect the starting strain CCTCC NO:M 203052 glycerol tube into the shake flask filled with YPD culture solution, add 0.01% 5-fluorouracil, and culture at 30°C and 200rpm for 16-24h with shaking. Take the well-grown culture medium to collect the bacteria by centrifugation and wash with saline several times, and finally suspend the cells with an equal amount of LiCl aqueous solution. Take an appropriate amount of cell suspension and place it in a sterile plate to irradiate mutagenesis with ultraviolet light, the power of the ultraviolet lamp is 15W, the irradiation distance is 20cm, and the irradiation time is 90s. The mutated strains were cultured on a plate containing YPD medium at a culture temperature of 29° C. for a culture time of 50 h, and picked well-grown single clones to a culture medium containing a primary screening medium (substrate long-chain alkanes were included in the culture medi...

Embodiment 2～7

[0090] Examples 2-7 Fermentation of Candida tropicalis CAT H1614 in a 10L fermenter to produce different dibasic acids

[0091] Get the glycerol tube bacterial classification of Candida tropicalis CAT H1614 and inoculate in the seed bottle that 30ml YPD liquid culture medium (glucose 2%, yeast extract 1%, peptone 2%) is housed, pH is natural, under 29 ℃, 220rpm shaker Cultured for 1 day. Get shake flask seed access and be equipped with 5L seed culture medium (sucrose 2%, corn steep liquor 0.3%, yeast extract 0.5%, KH 2 PO 4 0.8%, urea 0.3%) in the seed tank, the inoculation amount is 10%, the initial pH value of the system after inoculation is 6.0, at 29°C, the ventilation rate is 0.4vvm, the tank pressure is 0.08MPa, and the culture is 18h. natural drop to 3, OD 620 When growing to 0.7, inoculate into 6L fermentation medium 1 (4% of glucose, 0.5% of corn steep liquor, 0.4% of yeast extract, 1% of potassium nitrate, 0.1% of potassium dihydrogen phosphate, 0.12% of urea, 0....

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Abstract

The invention discloses a candida tropicalis strain CAT H1614, a microbial agent comprising the same and an application thereof, and a method for producing long chain diprotic acid by fermentation anda preparation method of the long chain diprotic acid. The candida tropicalis strain CAT H1614 of the invention can maintain high vitality for a long time in an environment with a pH lower than 7.0, thereby being particularly suitable for production of the long chain diprotic acid by a biological method, acid production is rapid during fermentation, the yield is high, and a fermentation cycle canalso be reduced. The fermentation method and the preparation method of the long chain diprotic acid provided by the invention have significant cost advantages, can effectively alleviate pressure on resources and environment, and thus have very obvious industrialization value advantages.

Description

technical field [0001] The invention relates to the field of fermentation, in particular to Candida tropicalis and its application in the field of fermentation, and a production method of long-chain dibasic acid. Background technique [0002] The general formula of long chain dibasic acid (LCDA) is (HOOC(CH 2 )nCOOH, n≥7), has a very wide range of uses. Using long-chain dibasic acids as raw materials can synthesize special nylon, high-grade spices, high-grade hot-melt adhesives, cold-resistant plasticizers, high-grade lubricating oils, high-grade rust inhibitors, high-grade paints and coatings etc. Long-chain dibasic acids can usually be synthesized chemically or biologically. The synthetic route of the chemical method is long, the reaction requires high temperature and high pressure, and the requirements for the catalyst are relatively strict. Therefore, there are few long-chain dibasic acids on an industrial scale, and there are only a few varieties such as twelve-carbon...

Claims

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Application Information

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IPC IPC(8): C12N1/16C12P7/64C12R1/74
CPCC12N1/16C12P7/6409C12R2001/74C12N1/165
Inventor 徐敏郝英利杨晨秦兵兵李乃强刘修才
Owner CATHAY R&D CENT CO LTD
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