Method for improving fermentation yield of long-chain dicarboxylic acid

A technology of long-chain dibasic acid and microbial fermentation method is applied in the field of improving long-chain dibasic acid fermentation yield, which can solve the problems of large workload of strain improvement, difficult operation and control, and many uncertain factors, and achieves α- The effect of oxidative decarboxylation and β-oxidation is weak, the acid production rate is fast, and the unit consumption of alkane is reduced

Inactive Publication Date: 2012-12-05
ZIBO GUANGTONG CHEM
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Problems solved by technology

[0006] The above-mentioned method mainly improves the fermentation yield from the aspect of strain improvement, but strain improvement has a large workload, many uncertain factors, and is difficult to operate and master. The fermentation yield is still low, generally reaching 35% (molar conversion rate), still to be further improved

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment l

[0028] The method for improving the fermentation yield of long-chain dibasic acid comprises the following steps:

[0029] (1) Take an inoculation loop of Candida tropicalis, spread it on the solid slant medium of a large test tube of 20x200mm, and cultivate it at 30°C for 48 hours. The slant medium is solid slant medium (g / L): yeast extract 5, white sugar 10, peptone 5 and agar 15.

[0030] (2) Transfer the bacteria cultured in step (1) into a 500mL Erlenmeyer shaker flask containing 15mL of seed medium, and cultivate for 40 hours at 30°C on a rotary shaker at 180 rpm.

[0031] Seed medium (g / L): White sugar 5, potassium dihydrogen phosphate 8, yeast extract 1.5, corn steep liquor 1.5, urea 1.8, 200# heavy wax oil 30ml / L, prepared with tap water, natural pH.

[0032] (3) In a 500mL Erlenmeyer shaker flask containing 15mL of fermentation medium, add 5mI of the above seed solution, and at the same time add 0.5mmol / L inhibitor chlorpromazine hydrochloride, and make 3 parallel sa...

Embodiment 2

[0036] By the method of embodiment 1, replace chlorpromazine hydrochloride with inhibitor phenobarbital sodium, add when shaking flask inoculation, concentration is 1mmol / L, and fermentation substrate is nC 13 , the amount added is 3ml. The residual hydrocarbon is 0 and the fermentation is over. The dibasic acid is measured by the method of Example 1, and the conversion rate of the fermented alkane is obtained through calculation. ) reached 96.8wt.%, the total acid content (neutralization titration) was 99.1wt.%, and the molar conversion of alkane reached 58.0%.

Embodiment 3

[0038] According to the method of Example 1, replace chlorpromazine hydrochloride with inhibitor acrylic acid, and add when cell culture 28 hours, concentration is 0.05mmol / L, fermentation substrate is nC l4 , the amount added is 3ml. The residual hydrocarbon is 0 and the fermentation ends. After the fermentation, the method of Example 1 was used to measure the dibasic acid, and the conversion rate of the fermented alkane was calculated. As a result, the amount of acid produced was 162.5g / L, and 1.9013g of tetradecanedioic acid was extracted, and the monoacid content (GC) reached 97.5 wt.%, the total acid content (neutralization titration) was 99.4wt.%, and the molar conversion of alkane reached 64%.

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Abstract

The invention belongs to the technical field of chemical products, and relates to a method for improving the fermentation yield of long-chain dicarboxylic acid. According to the method, the long-chain dicarboxylic acid is produced by a microbiological fermentation method by taking C10 to C18 n-alkanes as raw materials; the method is characterized in that alpha oxidative decarboxylation inhibitor and beta-oxidation inhibitor are added in a fermentation production process to reduce the alpha-oxidative decarboxylation and beta-oxidation capacities of strains, wherein the alpha oxidative decarboxylation inhibitor is one or mixture of more of chlorpromazine hydrochloride, phenobarbital sodium, crylic acid and polyacrylic acid, and the concentration is 0.01 to 1mmol / L; and the beta-oxidation inhibitor is one or mixture of more of mercaptoacetic acid, sodium thioglycollate, ranolazine, ranolazine dihydrochloride and mildronate, and the concentration is 0.01 to 1 mmol / L. Compared with the prior art, the method has the advantages of high acid producing speed, weak alpha oxidative decarboxylation and beta-oxidation capacity, low unit consumption of alkane, and high fermentation alkane conversion rate, and is particularly suitable for preparing the long-chain dicarboxylic acid.

Description

technical field [0001] The invention belongs to the technical field of chemical product preparation, and relates to a method for increasing the fermentation yield of long-chain dibasic acids. Background technique [0002] Long-chain dibasic acid is an important fine chemical raw material, which can be used in the synthesis of spices, hot melt adhesives, engineering plastics and liquid crystals, etc., and is widely used. [0003] At present, long-chain dibasic acids are mainly produced by microbial fermentation, and the special function of microbial double-end oxidation is used to ferment normal alkanes to produce long-chain dibasic acids. According to the mechanism study of microbial oxidation of alkanes to long-chain dibasic acids, the main reaction is α, ω-oxidation, and the side reactions are α-oxidative decarboxylation and β-oxidation, and the long-chain dibasic acids produced can be further oxidatively degraded . Although the α, ω-oxidation ability of domestic and for...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P7/64C12R1/74
Inventor 苏庆旺尹海顺刘文
Owner ZIBO GUANGTONG CHEM
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