Method for preparing angiotensin converting enzyme (ACE) inhibitory peptide from sea cucumber
A technology for inhibiting peptides and sea cucumbers is applied in the field of preparing ACE-inhibiting peptides by using sea cucumbers to achieve the effects of reasonable method and process, high utilization value and reducing diastolic blood pressure.
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Embodiment 1
[0022] A method for preparing ACE inhibitory peptides from sea cucumbers, the specific process steps are as follows:
[0023] a. Selection and processing of raw materials Select 100kg of fresh sea cucumbers as raw materials, remove the intestines, put them in a boiling water bath and boil for 20 minutes, remove the water and remove the ginseng mouth, and then use a 0.1Mpa 121°C for 30 minutes for softening treatment. The sea cucumber is 7kg, and the softened sea cucumber is beaten using a beater, and then the material is further refined by using a colloid mill to obtain a very small sea cucumber slurry;
[0024] b. Enzymolysis Add the obtained sea cucumber slurry to 200L of pure water, heat until the solution center temperature reaches 100°C, and continue for 10 minutes to eliminate microorganisms; cool to 50°C, add 40g of compound proteolytic enzyme, and enzymolyze for 2h to obtain Enzymolysis solution; wherein the composite proteolytic enzyme can be a commercially available ...
Embodiment 2
[0031] A method for preparing ACE inhibitory peptides from sea cucumbers, the specific process steps are as follows:
[0032] a. Selection and processing of raw materials: choose 5 kg of dried sea cucumber as raw material, soak in cold water for 1 day, remove and boil in boiling water for 20 minutes, and then use a sterilizing pot at 0.1Mpa 121°C for 20 minutes for softening treatment. The sea cucumber is beaten by a beater, and then the material is further refined by a colloid mill to obtain a sea cucumber slurry with a small particle size;
[0033] b. Enzymolysis: add the obtained sea cucumber slurry into 100L of pure water, heat until the solution center temperature reaches 95°C, and continue for 30 minutes to eliminate microorganisms; cool to 55°C, add flavor proteolytic enzyme 50g, and enzymatically hydrolyze for 1 hour, Obtain enzymatic solution;
[0034] c. Enzyme inactivation Heat the enzymolysis solution until the center temperature of the solution reaches 95°C for 1...
Embodiment 3
[0040] A method for preparing ACE inhibitory peptides from sea cucumbers, the specific process steps are as follows:
[0041] a. Selection and processing of raw materials It is to select 20kg of water ginseng as raw material, use a beater to perform beating treatment, and then use a colloid mill to further refine the material to obtain a sea cucumber slurry with a very small particle size;
[0042]b. Enzymolysis: add the obtained sea cucumber slurry to 200L of pure water, heat until the center temperature of the solution reaches 100°C, hold for 15min, and kill microorganisms; cool to 50°C, add 50g of compound proteolytic enzyme, and enzymatically hydrolyze for 2h , to obtain the enzymatic solution; the composite proteolytic enzyme is commercially available Protamex composite protease;
[0043] c. Enzyme inactivation Heat the enzymolysis solution until the center temperature of the solution reaches 100°C for 10 minutes to inactivate the enzyme;
[0044] d. Removal of insoluble...
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