Method for preparing angiotensin converting enzyme (ACE) inhibitory peptide from sea cucumber

A technology for inhibiting peptides and sea cucumbers is applied in the field of preparing ACE-inhibiting peptides by using sea cucumbers to achieve the effects of reasonable method and process, high utilization value and reducing diastolic blood pressure.

Active Publication Date: 2012-06-27
山东海普盾生物科技有限公司
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  • Summary
  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

At present, there is no research report on the preparation of ACE inhibitory peptides from sea cucumbers

Method used

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Examples

Experimental program
Comparison scheme
Effect test

Embodiment 1

[0022] A method for preparing ACE inhibitory peptides from sea cucumbers, the specific process steps are as follows:

[0023] a. Selection and processing of raw materials Select 100kg of fresh sea cucumbers as raw materials, remove the intestines, put them in a boiling water bath and boil for 20 minutes, remove the water and remove the ginseng mouth, and then use a 0.1Mpa 121°C for 30 minutes for softening treatment. The sea cucumber is 7kg, and the softened sea cucumber is beaten using a beater, and then the material is further refined by using a colloid mill to obtain a very small sea cucumber slurry;

[0024] b. Enzymolysis Add the obtained sea cucumber slurry to 200L of pure water, heat until the solution center temperature reaches 100°C, and continue for 10 minutes to eliminate microorganisms; cool to 50°C, add 40g of compound proteolytic enzyme, and enzymolyze for 2h to obtain Enzymolysis solution; wherein the composite proteolytic enzyme can be a commercially available ...

Embodiment 2

[0031] A method for preparing ACE inhibitory peptides from sea cucumbers, the specific process steps are as follows:

[0032] a. Selection and processing of raw materials: choose 5 kg of dried sea cucumber as raw material, soak in cold water for 1 day, remove and boil in boiling water for 20 minutes, and then use a sterilizing pot at 0.1Mpa 121°C for 20 minutes for softening treatment. The sea cucumber is beaten by a beater, and then the material is further refined by a colloid mill to obtain a sea cucumber slurry with a small particle size;

[0033] b. Enzymolysis: add the obtained sea cucumber slurry into 100L of pure water, heat until the solution center temperature reaches 95°C, and continue for 30 minutes to eliminate microorganisms; cool to 55°C, add flavor proteolytic enzyme 50g, and enzymatically hydrolyze for 1 hour, Obtain enzymatic solution;

[0034] c. Enzyme inactivation Heat the enzymolysis solution until the center temperature of the solution reaches 95°C for 1...

Embodiment 3

[0040] A method for preparing ACE inhibitory peptides from sea cucumbers, the specific process steps are as follows:

[0041] a. Selection and processing of raw materials It is to select 20kg of water ginseng as raw material, use a beater to perform beating treatment, and then use a colloid mill to further refine the material to obtain a sea cucumber slurry with a very small particle size;

[0042]b. Enzymolysis: add the obtained sea cucumber slurry to 200L of pure water, heat until the center temperature of the solution reaches 100°C, hold for 15min, and kill microorganisms; cool to 50°C, add 50g of compound proteolytic enzyme, and enzymatically hydrolyze for 2h , to obtain the enzymatic solution; the composite proteolytic enzyme is commercially available Protamex composite protease;

[0043] c. Enzyme inactivation Heat the enzymolysis solution until the center temperature of the solution reaches 100°C for 10 minutes to inactivate the enzyme;

[0044] d. Removal of insoluble...

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PUM

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Abstract

The invention relates to a method for preparing an angiotensin converting enzyme (ACE) inhibitory peptide from sea cucumber. The method comprises the following steps of: de-salting and softening raw materials including fresh sea cucumber, or dry sea cucumber, or soaked sea cucumber, or high-pressure sea cucumber; after preparing slurry, adding water into the slurry; heating the slurry to kill microorganisms; after cooling the slurry, adding composite proteolytic enzyme or flavor proteolytic enzyme into the slurry to perform enzymolysis; killing enzymes; after cooling, centrifuging the product to remove undissolved substances; filtering the supernatant fluid in a pipe; filtering the filtrate with a 3,000 Dalton organic membrane to trap and classify; and performing nano filtration and drying on the trapped liquid which is less than 3,000 Dalton in mass to obtain the ACE inhibitory peptide. A zymolytic technical scheme is optimized; and due to the separation and extraction technology of pipe filtration, ultrafiltration and nano filtration, the extraction efficiency is high, and the pressure-reducing effect of the obtained ACE inhibitory peptide is good. The method is reasonable in process, simple and convenient in operation and high in operability; and industrial production is easy to realize.

Description

technical field [0001] The invention relates to the preparation of a peptide, in particular to a method for preparing ACE inhibitory peptides from sea cucumbers. Background technique [0002] Antihypertensive peptides, also known as angiotensin converting enzyme (Angiotensin Converting Enzyme, EC3.4.15.1, ACE for short) inhibitory peptides, are a class of peptides isolated from food proteins that can significantly lower blood pressure. molecular peptide. Among the many antihypertensive substances, antihypertensive peptides have become a hotspot in the research of active peptides because of their non-toxic and side effect antihypertensive function. Now it has been obtained from casein, whey protein, etc. in cheese protein; chicken, gelatin, pork, eggs, buckwheat, corn, broccoli, garlic, etc. in animal and vegetable protein; sardines, tuna, eel in fish and shellfish algae protein ACE-inhibiting peptides were isolated from head, oyster, wakame, etc.; some domestic researchers...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C12P21/06
Inventor 袁文鹏孙永军胡炜刘昌衡王志伟张绵松刘新唐聚德
Owner 山东海普盾生物科技有限公司
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