Puffer fish bioactive peptide and preparation method thereof

A technology of active peptide and pufferfish enzymatic hydrolyzate, which is applied in the field of ACE inhibitory peptides to achieve the effect of improving the inhibitory effect and high stability
CN110964087AActive Publication Date: 2020-04-07FISHERIES RES INST OF FUJIAN
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Patent Information

Authority / Receiving Office
CN · China
Patent Type
Applications(China)
Current Assignee / Owner
FISHERIES RES INST OF FUJIAN
Publication Date
2020-04-07

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Abstract

The invention discloses a puffer fish bioactive peptide and a preparation method thereof and belongs to the technical field of ACE (Angiotensin Converting Enzyme) inhibitory peptides. The preparationmethod of a puffer fish bioactive peptide comprises the following steps: S1, preparation of a puffer fish enzymatic hydrolysate; S2, ultrafiltration separation of the puffer fish enzymatic hydrolysate; S3, separation and purification of polypeptide; S4, sequence identification of polypeptide; and S5, polypeptide synthesis and activity determination. According to the puffer fish bioactive peptide,the bioactive peptide is octapeptide, and the amino acid sequence of the puffer fish bioactive peptide is PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu). According to the puffer fish bioactive peptide andthe preparation method thereof, the puffer fish skin is adopted as a raw material, an octapeptide amino acid sequence is obtained, and the prepared polypeptide has the ACE inhibitory activity.
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Description

technical field

[0001] The invention belongs to the technical field of ACE inhibitory peptides, in particular to a puffer fish active peptide and a preparation method thereof. Background technique

[0002] With the progress and development of society, people's living standards are gradually improving, and the proportion of fat and calories in the diet is gradually increasing. Coupled with the reduction in exercise, the incidence of cardiovascular diseases in my country has risen rapidly, of which hypertension is the main one.

[0003] Pufferfish is a bottom fish in the warm temperate zone and tropical coastal waters. It inhabits the middle and lower layers of the ocean. A few species enter freshwater rivers. When encountering external danger, the whole body floats to the surface in a spherical shape, and at the same time, the small spines on the skin stand up. , for self-defense.

[0004] Angiotensin-converting enzyme (ACE) inhibitory peptide is a small molecular polypeptid...

Claims

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