Puffer fish bioactive peptide and preparation method thereof
A technology of active peptide and pufferfish enzymatic hydrolyzate, which is applied in the field of ACE inhibitory peptides to achieve the effect of improving the inhibitory effect and high stability
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[0034] The present invention will be further described in conjunction with the accompanying drawings and specific embodiments.
[0035] The puffer fish active peptide provided in this example is an octapeptide, and its amino acid sequence is PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu).
[0036] The molecular weight of the octapeptide PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu) is 840.5Da.
[0037] This embodiment also provides a preparation method of puffer fish active peptide, comprising the following steps:
[0038]S1: Preparation of puffer fish enzymatic hydrolyzate, after beating 100g puffer fish skin, add 300mL distilled water and stir to form protein solution, adjust pH value to 8.0 with NaOH, then add alkaline protease, the enzyme amount is 500U / g, Enzyme hydrolyze at 55°C for 10 hours, keep the pH value unchanged, heat at 90°C for 15 minutes after enzymolysis, then cool to room temperature, adjust the pH value to neutral with HCl, then use 5000r / min After speed centrif...
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