Puffer fish bioactive peptide and preparation method thereof

A technology of active peptide and pufferfish enzymatic hydrolyzate, which is applied in the field of ACE inhibitory peptides to achieve the effect of improving the inhibitory effect and high stability

Active Publication Date: 2020-04-07
FISHERIES RES INST OF FUJIAN
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  • Abstract
  • Description
  • Claims
  • Application Information

AI Technical Summary

Problems solved by technology

[0005] There are existing technologies for preparing ACE-inhibiting peptides from hazelnuts, yak milk, shellfish, wolfberry and other foods, but there is no technology for preparing ACE-inhibiting peptides from puffer fish, especially puffer fish skin as raw materials

Method used

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  • Puffer fish bioactive peptide and preparation method thereof
  • Puffer fish bioactive peptide and preparation method thereof
  • Puffer fish bioactive peptide and preparation method thereof

Examples

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Embodiment Construction

[0034] The present invention will be further described in conjunction with the accompanying drawings and specific embodiments.

[0035] The puffer fish active peptide provided in this example is an octapeptide, and its amino acid sequence is PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu).

[0036] The molecular weight of the octapeptide PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu) is 840.5Da.

[0037] This embodiment also provides a preparation method of puffer fish active peptide, comprising the following steps:

[0038]S1: Preparation of puffer fish enzymatic hydrolyzate, after beating 100g puffer fish skin, add 300mL distilled water and stir to form protein solution, adjust pH value to 8.0 with NaOH, then add alkaline protease, the enzyme amount is 500U / g, Enzyme hydrolyze at 55°C for 10 hours, keep the pH value unchanged, heat at 90°C for 15 minutes after enzymolysis, then cool to room temperature, adjust the pH value to neutral with HCl, then use 5000r / min After speed centrif...

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Abstract

The invention discloses a puffer fish bioactive peptide and a preparation method thereof and belongs to the technical field of ACE (Angiotensin Converting Enzyme) inhibitory peptides. The preparationmethod of a puffer fish bioactive peptide comprises the following steps: S1, preparation of a puffer fish enzymatic hydrolysate; S2, ultrafiltration separation of the puffer fish enzymatic hydrolysate; S3, separation and purification of polypeptide; S4, sequence identification of polypeptide; and S5, polypeptide synthesis and activity determination. According to the puffer fish bioactive peptide,the bioactive peptide is octapeptide, and the amino acid sequence of the puffer fish bioactive peptide is PPLLFAAL (Pro-Pro-Leu-Leu-Phe-Ala-Ala-Leu). According to the puffer fish bioactive peptide andthe preparation method thereof, the puffer fish skin is adopted as a raw material, an octapeptide amino acid sequence is obtained, and the prepared polypeptide has the ACE inhibitory activity.

Description

technical field [0001] The invention belongs to the technical field of ACE inhibitory peptides, in particular to a puffer fish active peptide and a preparation method thereof. Background technique [0002] With the progress and development of society, people's living standards are gradually improving, and the proportion of fat and calories in the diet is gradually increasing. Coupled with the reduction in exercise, the incidence of cardiovascular diseases in my country has risen rapidly, of which hypertension is the main one. [0003] Pufferfish is a bottom fish in the warm temperate zone and tropical coastal waters. It inhabits the middle and lower layers of the ocean. A few species enter freshwater rivers. When encountering external danger, the whole body floats to the surface in a spherical shape, and at the same time, the small spines on the skin stand up. , for self-defense. [0004] Angiotensin-converting enzyme (ACE) inhibitory peptide is a small molecular polypeptid...

Claims

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Application Information

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Patent Type & Authority Applications(China)
IPC IPC(8): C07K7/06C12P21/06C07K1/36C07K1/34C07K1/16C07K1/04G01N33/68
CPCC07K7/06C12P21/06G01N33/6818G01N33/6848
Inventor 苏永昌刘智禹刘淑集陈贝乔琨许旻陈晓婷
Owner FISHERIES RES INST OF FUJIAN
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