Method for preparing angiotensin-I-converting enzyme (ACE) inhibitory peptide by using enzymolysis goat milk casein
A technology of milk casein and casein, which is applied in the field of preparation of milk-derived ACE inhibitory peptides, to achieve the effects of improving catalytic activity, no toxic side effects, and easy hydrolysis process
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Embodiment 1
[0014] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, use a high-speed centrifuge to centrifuge at 6400 rpm for 15 minutes, remove fat, and obtain skim milk;
[0015] Step 2: heat the obtained skim milk in a constant temperature water bath at 35°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution reaches 4.6, and make the cheese Precipitate the protein, centrifuge at 6400rpm for 15min with a high-speed centrifuge, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 12h, and then transfer to a freeze dryer at -55°C Freeze-dried for 24 hours to obtain casein freeze-dried powder. It was determined that when the skim milk was at 35°C, pH 4.6, and stirring speed 800 rpm, the yield of casein was the highest, reaching 9.23%.
Embodiment 2
[0017] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, use a high-speed centrifuge to centrifuge at 6400 rpm for 15 minutes, remove fat, and obtain skim milk;
[0018] Step 2: heat the obtained skim milk in a constant temperature water bath at 45°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution reaches 4.5, and make the cheese Precipitate the protein, use a high-speed centrifuge at 6400rpm for 15min, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 20h, and then transfer to a freeze dryer at -55°C Freeze-dried for 24 hours to obtain casein freeze-dried powder. It was determined that when the skim milk was at 45°C, pH 4.5, and stirring speed 800 rpm, the casein yield was the highest, reaching 11.88%;
[0019] Step 3: Make 100ml of casein solution wit...
Embodiment 3
[0021] Step 1: Mix and dissolve goat milk powder and water according to the mass ratio of 1:9, cool the obtained reconstituted goat milk to 4°C, use a high-speed centrifuge to centrifuge at 6400 rpm for 15 minutes, remove fat, and obtain skim milk;
[0022] Step 2: heat the obtained skim milk in a constant temperature water bath at 45°C, stir with a magnetic heating stirrer at a stirring speed of 800 rpm, add HCl solution dropwise while stirring, so that the pH of the skim milk solution reaches 4.5, and make the cheese Precipitate the protein, use a high-speed centrifuge at 6400rpm for 15min, discard the supernatant, freeze-dry the precipitated casein, pre-freeze at -40°C for 24h, and then transfer to a freeze dryer at -55°C Freeze-dried for 24 hours to obtain casein freeze-dried powder. It was determined that when the skim milk was at 45°C, pH 4.5, and stirring speed 800 rpm, the casein yield was the highest, reaching 11.88%;
[0023] Step 3: Make the casein freeze-dried powd...
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